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3.5.5.8: thiocyanate hydrolase

This is an abbreviated version!
For detailed information about thiocyanate hydrolase, go to the full flat file.

Word Map on EC 3.5.5.8

Reaction

thiocyanate
+ 2 H2O =
carbonyl sulfide
+
NH3
+
HO-

Synonyms

SCNase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.8 thiocyanate hydrolase

Temperature Stability

Temperature Stability on EC 3.5.5.8 - thiocyanate hydrolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 80
-
the activity of the recombinant enzyme is completely retained after heat treatment at temperatures from 30 to 70°C, but is decreased by heating at 80°C for 5 min to 80%, to 60% at 90°C, and to 50% at 100°C
4
-
incubating recombinant enzyme on ice for 3 h reduces the enzyme activity to 2% of the original activity and subsequent heating at 60°C for 15 min returns the activity to the original level
60
-
treatment of the enzyme at 60°C for 5 min decreases activity below 10% of the original