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Literature summary for 3.5.5.8 extracted from

  • Arakawa, T.; Kawano, Y.; Katayama, Y.; Nakayama, H.; Dohmae, N.; Yohda, M.; Odaka, M.
    Structural basis for catalytic activation of thiocyanate hydrolase involving metal-ligated cysteine modification (2009), J. Am. Chem. Soc., 131, 14838-14843.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thiobacillus thioparus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) containing 2.0 M ammonium sulfate for purified SCNase or 0.1 M potassium phosphate (pH 6.9) containing 1.5 M Na/K tartrate for spontaneously activated SCNase and inactivated SCNase, respectively Thiobacillus thioparus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus O66186 subunit beta
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Thiobacillus thioparus O66187 subunit alpha
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Thiobacillus thioparus O66188 subunit gamma
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Oxidation Stability

Oxidation Stability Organism
when recombinant SCNase, containing gammaCys131-SO2 – and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected Thiobacillus thioparus
when recombinant SCNase, containing gammaCys131-SO2- and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected Thiobacillus thioparus

Purification (Commentary)

Purification (Comment) Organism
-
Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiocyanate + H2O
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Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
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Synonyms

Synonyms Comment Organism
SCNase
-
Thiobacillus thioparus