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3.5.5.8: thiocyanate hydrolase

This is an abbreviated version!
For detailed information about thiocyanate hydrolase, go to the full flat file.

Word Map on EC 3.5.5.8

Reaction

thiocyanate
+ 2 H2O =
carbonyl sulfide
+
NH3
+
HO-

Synonyms

SCNase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.8 thiocyanate hydrolase

Crystallization

Crystallization on EC 3.5.5.8 - thiocyanate hydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) containing 2.0 M ammonium sulfate for purified SCNase or 0.1 M potassium phosphate (pH 6.9) containing 1.5 M Na/K tartrate for spontaneously activated SCNase and inactivated SCNase, respectively
hanging drop vapour diffusion method with 0.1 M potassium phosphate (pH 7.6) containing 1.5 M Na/K L-(+) tartrate or 0.1 M potassium phosphate (pH 7.2) containing 2 M ammonium sulfate for apo- or native SCNase, respectively
-
mutant R136W, hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) and 1.4 M sodium potassium tartrate