3.2.1.8: endo-1,4-beta-xylanase

This is an abbreviated version!
For detailed information about endo-1,4-beta-xylanase, go to the full flat file.

Reaction

Synonyms

(1--> 4)-beta-xylan 4-xylanohydrolase, (1-4)-beta-xylan 4-xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase 22, 1,4-beta-D-xylan-xylanohydrolase, 1,4-beta-xylan xylanohydrolase, 1,4-beta-xylanase, 34 kDa xylanase, Abf51A, Abf62A-Axe6A, Acel_0180, acidophilic endo-1,4-beta-xylanase, AfXynA, AfXynB, alkaline active xylanase, alkaline xylanase, AMX-4 xylanase, AnxB, AxB8, Axy43A, basic xylanase, Bcx, beta-1, 4-endoxylanase, beta-1,4-D-xylanase, beta-1,4-endoxylanase, beta-1,4-xylan hydrolase, beta-1,4-xylan xylanohydrolase, beta-1,4-xylanase, beta-1,4endoxylanase, beta-D-xylanase, beta-endoxylanase, beta-xylanase, bifunctional cellulase/xylanase, bifunctional endoglucanase/xylanase, bifunctional xylanase/endoglucanase, BlxA, BSX, BSXY, Btx, Calow_0124, ctendo7, Cthe_3012, CTHT_0045780, CtXynGH30, EGXA, endo(1-4)beta-xylanase, endo-(1,4)-beta-xylanase, endo-(1--> 4)-beta-xylanase, endo-1,4-beta-D-xylanase, endo-1,4-beta-xylanase, endo-1,4-beta-xylanase II, endo-1,4-xylanase, endo-acting beta-1,4-xylanase, endo-beta-(1'4)-xylanase, endo-beta-(1,4)-xylanase, endo-beta-1, 4-xylanase, endo-beta-1,4-xylanase, endo-beta-1,4-xylanase 2, endo-beta-1,4-xylanase2, endo-xylanase, endoxylanase, endoxylanase I, endoxylanase NtSymX11, endoxylanase Xys1DELTA, EXY1, family 11 endoxylanase, family 11 xylanase, family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase, family-10 endo-1,4-beta-xylanase, FIA-xylanase, FOTG_15646, G/11 endo-1,4-beta-xylanase, GC25 xylanase, GH 10 xylanase, GH 11 xylanase, GH-10 endo-1,4-beta-xylanase, GH-11 endo-1,4-beta-xylanase, GH10 endo-beta-1,4-xylanase, GH10 xylanase D, GH11 xylanase, GH43B6, GH7 endoglucanase, GHF 10 endoxylanase, GHF 11 endoxylanase, glycoside hydrolase family 11 endoxylanase, glycoside hydrolase family 8 domain protein, GXYN, KRICT PX1, MalAC0309_0409, More, MROS_2090, MROS_2091, MROS_2495, Mxyn10, MYCTH_49824, MYCTH_56237, ORF4, Pedsa_2704, PhX20, PhX33, PsGH10A, pXyl, RrXyn11A, RuCelA, Rut C-30, SCO5931, SipoEnXyn10A, SlxB, SoxB, SSO1354 protein, TAXI, TERTU_4506, Tfu_1213, TLX, TmxB, Tpet_0854, TRX II, TtGH8, Wxl1, X-I, X-II, X34, Xa, Xln-1, xlnA, xlnB, XT6, Xyl, Xyl I, Xyl II, XYL1, Xyl10A, Xyl10B, XYL10C, Xyl11, XYL1p, XYL2, Xyl2090, Xyl2091, Xyl2495, Xyl30, XYLA, xylanase, xylanase 1, xylanase 10A, xylanase 10B, xylanase 10C, xylanase 11 A, xylanase 11A, xylanase 11J, xylanase 2, Xylanase 22, xylanase 43A, xylanase A, xylanase B, xylanase bI, xylanase bII, xylanase C, xylanase I, xylanase II, xylanase III, xylanase J, xylanase LC9, xylanase Xyl10A, xylanase Xyl11A, xylanase XynZF-2, xylanase, endo-1,4-, XylB, XylB8, XylC, XYLD, XylE, XylF2, XylG, xylH, xylM, XylX, XYLY, Xyn II, Xyn III, Xyn-b39, Xyn-Lxy, Xyn1, Xyn10, Xyn10A, Xyn10B, Xyn10E, XYN10G5, XYN10Ks_480, Xyn11, Xyn11A, XYN11F63, XYN11Ks_480, Xyn11NX, Xyn12.2, Xyn162, Xyn2, Xyn3, Xyn30A, Xyn5, XynA, XynA119, XynA19, XynA4, XynAS27, XynAS9, XynB, XynB18, XynBS27, XynC, XynD, XynE15, XynE2, XynG1, XynGH30, XynGR40, XYNII, XynIII, XynJ, XynS14, XynS20, XynSW1, XynSW3, XynT, XynY, XynZ, Xys1, Xys1delta, xysA

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.8 endo-1,4-beta-xylanase

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Results	in table
137	Activating Compound
22184	AA Sequence
190	Application
1	CAS Registry Number
186	Cloned(Commentary)
31	Crystallization (Commentary)
24	Expression
101	General Information
12	General Stability
825	Inhibitors
50	kcat/KM [mM/s]
9	Ki Value [mM]
201	KM Value [mM]
171	Localization
205	Metals/Ions
358	Molecular Weight [Da]
97	Natural Substrates/ Products (Substrates)
14	Organic Solvent Stability
978	Organism
4	Pathway
644	PDB ID
408	pH Optimum
136	pH Range
214	pH Stability
89	pI Value
103	Posttranslational Modification
251	Protein Variants
244	Purification (Commentary)
16	Reaction
7	Reaction Type
744	Reference
2	Renatured (Commentary)
86	Source Tissue
521	Specific Activity [micromol/min/mg]
18	Storage Stability
1769	Substrates and Products (Substrate)
389	Subunits
968	Synonyms
1	Systematic Name
385	Temperature Optimum [°C]
120	Temperature Range [°C]
469	Temperature Stability [°C]
165	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.2.1.8 - endo-1,4-beta-xylanase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.6 - 10.3

Streptomyces sp.

F8WSY7

-

729010

1 - 10

Cryptococcus sp. (in: Fungi)

-

1 h, 30°C, 19% loss of activity at pH 1, 90% loss of activity at pH 10.0

171583

1 - 6

Bispora sp. MEY-1

D6MYS9

purified recombinantg enzyme, 37°C, stable

707258

1.5 - 11.5

Streptomyces sp.

-

xylanase X-II-A and X-II-B, stable

171592, 171594

10

6 entries

11

5 entries

2 - 10

Penicillium sp. 'occitanis'

-

the enzyme retains 100% of its activity over a wide pH range from 2.0 to 10.0

718338

2 - 11

2 entries

2 - 12

2 entries

2 - 6

Bacillus sp. (in: Bacteria)

-

stable

171628

2 - 9

Pseudozyma hubeiensis

-

PhX20, 90% activity remains after 48 h

707766

2.2 - 11

Aspergillus niger

-

22°C, 2 h, 75% residual activity

731024

2.2 - 12

Streptomyces sp. S27

C3RYK8

-

695838

2.4

Aspergillus sulphureus

Q3S401

purified recombinant enzyme, over 90% activity remaining after 2 h

707197

2.5 - 6

Malbranchea pulchella

-

xylanase A, stable

171587

2.5 - 9.5

Talaromyces pinophilus

-

isozyme III, completely stable at 25°C, 2 h

655807

2.6 - 12

Alicyclobacillus sp.

D3VWB5

purified recombinant XynA4, 1 h, 37°C, over 80% remaining activity

717947

3

7 entries

3 - 10

4 entries

3 - 10.5

2 entries

3 - 11

2 entries

3 - 8

2 entries

3 - 9

9 entries

3 - 9.5

Talaromyces pinophilus

-

isozyme II, completely stable at 25°C, 2 h

655807

3.5 - 10

Demequina sp. JK4

B9VSZ3

purified recombinant enzyme, stable

709491

3.5 - 10.5

Aspergillus flavus

B8NKE9

purified extracellular enzyme, stable at

752113

3.5 - 5.5

Thermothelomyces thermophilus

-

-

742765

3.5 - 7

Clostridium acetobutylicum

-

xylanase B, stable

171603

3.5 - 7.5

Trichoderma reesei

B2CNY5

purified recombinant enzyme, stable

709576

3.5 - 8

Malbranchea pulchella

-

xylanase B, stable

171587

3.5 - 8.5

2 entries

3.5 - 9.5

Talaromyces pinophilus

-

isozyme I, completely stable at 25°C, 2 h

655807

4

6 entries

4 - 10

6 entries

4 - 10.5

Malbranchea cinnamomea

A0A088BC14

-

742680

4 - 11

4 entries

4 - 12

2 entries

4 - 6.7

Aspergillus nidulans

-

20 h, more than 80% of maximal activity remains

171658

4 - 7

2 entries

4 - 7.5

Penicillium griseofulvum

-

retains full activity for 2 h at 30°C

701124

4 - 8

2 entries

4 - 9

3 entries

4 - 9.5

2 entries

4.5

2 entries

4.5 - 10

Bacillus sp. (in: Bacteria)

-

1 h, 45°C, stable between pH 4.5 and 10.0, enzyme component I and II from strain W1 and W2, stable

171591, 171595

4.5 - 10.5

2 entries

4.5 - 11.5

Caldicellulosiruptor saccharolyticus

-

stable at room temperature in presence of 0.5 mg/ml bovine serum albumin

171655

4.5 - 7

2 entries

4.5 - 9

2 entries

4.5 - 9.5

Pomacea insularus

-

35°C, 10 min, stable

171584

5

7 entries

5 - 10

8 entries

5 - 11

5 entries

5 - 12

3 entries

5 - 6

2 entries

5 - 7

4 entries

5 - 7.5

2 entries

5 - 8

5 entries

5 - 8.5

Pomacea insularus

-

40°C, 30 min, enzyme retains more than 85% of maximal activity at pH 5.0 and at pH 8.5

171584

5 - 9

3 entries

5.4

Naganishia albida

-

50°C, 0.1 M NaCl, half-life: 7 min

171585

5.5

4 entries

5.5 - 10

Saccharopolyspora pathumthaniensis

E1APH5

purified enzyme, stable

717401

5.5 - 10.5

Halalkalibacterium halodurans

-

fully stable from pH 5.5 to 10.5 after 12 h of incubation at 50°C

679547

5.5 - 5.6

Aspergillus niger

-

48°C, 50% loss of activity after 40 h

171598

5.5 - 7

Clostridium acetobutylicum

-

xylanase A, stable

171603

5.5 - 7.5

Pseudothermotoga thermarum

F7YXD6

50°C, stable for 1 h

729475

5.5 - 8.5

Thermochaetoides thermophila

Q8J1V6

purified recombinant enzyme, quite stable

709894

5.5 - 9

Thermomyces lanuginosus

-

stable for 30 min

742766

6

3 entries

6 - 10

5 entries

6 - 10.5

Bacillus sp. (in: Bacteria)

-

-

667012

6 - 8

3 entries

6 - 9

4 entries

6.5

3 entries

6.5 - 12

Thermomyces lanuginosus

-

-

696797

6.7

Malbranchea pulchella

-

50°C, 30 min, stable

171587

7

5 entries

7 - 12

Thermoclostridium stercorarium

-

4°C, 24 h, stable

171615

7 - 8

2 entries

7 - 9

Priestia megaterium

-

-

681810

7.5

2 entries

7.5 - 9.5

Staphylococcus sp.

-

50°C, 1 h, retains more than 90% of its activity

171641

7.6 - 12

Kocuria sp. Mn22

B8XY24

purified recombinant enzyme, over 80% remaining activity after overnight incubation

709503

8

6 entries

8 - 10

Bacillus sp. (in: Bacteria)

-

stable for 3 h at 50°C

654996

8.3

Thermomyces lanuginosus

-

wild-type and mutant enzymes, stable up to 24 h at 50°C, 60% remaining activity

655088

8.5

2 entries

9

9 entries

additional information

2 entries

10

Bacillus amyloliquefaciens

-

50°C, 5 h, less than 20% loss of activity

171646

10

Bacillus sp. (in: Bacteria)

-

purified enzyme, 4 h, 47% activity remaining

717091

10

Geobacillus sp. WBI

B5M201

1 h, 65°C, 100% residual activity

738547

10

Halalkalibacterium halodurans

-

no detectable activity after 1 h at pH 10 at 65°C

679547

10

Paenibacillus sp.

A0A075EEX4

45°C, 2 h, 85% residual activity

730187

10

Thermotoga petrophila

A5IL00

reduction to about 20% residual enzymatic activity

748630

11

Aspergillus terreus

-

90 min, enzyme retains 38.75% of its initial activity

750795

11

Bacillus licheniformis

-

X-I and X-II retain more than 75% of hydrolytic activity up to pH 11.0

677623

11

Fusarium commune

A0A0F7MX47, A0A0F7MYK0, A0A0F7N1T1

1 h, more than 90% residual activity

738135

11

Geobacillus sp. WBI

B5M201

1 h, 65°C, 75% residual activity

738547

11

Thermotoga petrophila

A5IL00

reduction to about 20% residual enzymatic activity

748630

2 - 11

Streptomyces olivaceoviridis

-

30°C, 1 h, stable

171659

2 - 11

Streptomyces sp.

F7J663

4°C, 16 h, more than 80% of initial activity

730370

2 - 12

Thermothelomyces thermophilus

G2QDB9, G2QIK8

purified recombinant enzyme, 60 min, room temperature, MYCTH_49824 retains nearly 70% activity

750646

2 - 12

Thermothelomyces thermophilus

G2QDB9, G2QIK8

purified recombinant enzyme, 60 min, room temperature, MYCTH_56237 retains nearly 70% activity

750646

3

Bacillus subtilis

P18429

30-70% remaining activity for the enzyme mutants, 23% for the wild-type

710466

3

Pediococcus acidilactici

-

purified extracellular enzyme, 85% activity remaining after 24 h, 50% activity remaining after 168 h

750814

3

Streptomyces halstedii

-

32°C, 1 h, complete inactivation

171639

3

Thermoclostridium stercorarium

-

4°C, 24 h, xylanase B and C lose 50% of their activity

171615

3

Thermotoga petrophila

A5IL00

reduction to about 20% residual enzymatic activity

748630

3

uncultured microorganism

E2DQY5

purified recombinant RuCelA, 24 h, 55% activity remaining

717134

3

uncultured microorganism

E2DQY5

xylan as substrate, loses 45% of its activity after incubation for 24 h

717134

3 - 10

Paecilomyces variotii

-

stable

738999

3 - 10

Streptomyces sp.

-

Xyl1-Xyl3, no loss of activity in 7 days at 20°C

655355

3 - 10

Termitomyces clypeatus

-

1 h, stable

171630

3 - 10

Trichoderma sp.

-

stability profile of EX1 and EX2, highly to completely stable at pH 4.0 to pH 9.0, inactivation at pH 3.0 and pH 10.0, overview

707762

3 - 10.5

Streptomyces sp.

-

xylanase X-I, stable

171592, 171594

3 - 10.5

Streptomyces thermocyaneoviolaceus

Q9RMM4

purified enzyme, stable at pH 4.5-10.5, 30% remaining at pH 3.0, overview

707809

3 - 11

Thermoclostridium stercorarium subsp. thermolacticum

-

4°C, 20 h, stable

171649

3 - 11

Thermomyces dupontii

-

-

742661

3 - 8

Aspergillus niger

-

stable

171634

3 - 8

Phanerodontia chrysosporium

-

isoforms xynA, xynB, stable for at least 2 h

664683

3 - 9

Aspergillus niger

I3QKR9

1 h, 40°C, mutant enzyme T205C/A52C is stable

751243

3 - 9

Penicillium citrinum

Q2PGY1

purified recombinant extracellular deglycosylated enzyme, 24 h, over 80% activity remains

752115

3 - 9

Phialophora sp. CGMCC 3328

F2VRY7

XYN10G, 70% of maximal activity within this range

717131

3 - 9

Pseudozyma hubeiensis

-

PhX33, 90% activity remains after 48 h

707766

3 - 9

Rasamsonia byssochlamydoides

-

4°C, 24 h, xylanase X-a and X-b-II, stable

171627

3 - 9

Rasamsonia byssochlamydoides

-

stable, xylanase a

738999

3 - 9

Rasamsonia byssochlamydoides

-

stable, xylanase bII

738999

3 - 9

Streptomyces avermitilis

Q9X584

purified heavier protein band (42.8 kDa), retains more than 50% of its activity

698373

3 - 9

Thermomyces lanuginosus

-

-

742958

3.5 - 8.5

Rasamsonia byssochlamydoides

-

4°C, 24 h, xylanase X-b-I, stable

171627

3.5 - 8.5

Rasamsonia byssochlamydoides

-

stable, xylanase bI

738999

4

Actinomadura sp.

-

XynS14 expressed from Pichia pastoris shows 50% remaining activity after 1 h, the enzyme expressed from Escherichia coli shows 10% remaining activity after 30 min

717886

4

Bacillus subtilis

P18429

75-95% remaining activity for the enzyme mutants, 57% for the wild-type

710466

4

Saccharolobus solfataricus

-

shows 46% maximal activity at pH 4.0

679721

4

Thermoclostridium stercorarium

-

4°C, 24 h, complete inactivation of xylanase A

171615

4

Thermopolyspora flexuosa

-

recombinant His-tagged enzyme, 30 min, 65°C, wild-type enzyme: half-life is 18 min with substrate, 3.1 min without substrate, isolated catalytic domain: life is 15 min with substrate, 1.3 min without substrate

707229

4

Thermothelomyces heterothallicus

-

63% remaining activity

655176

4 - 10

Penicillium citrinum

-

-

681711

4 - 10

Streptomyces fradiae

A7TVD4

after incubation at 37°C for 1 h

699489

4 - 10

Streptomyces halstedii

-

32°C, 1 h, at least 80% of the activity maintained

171639

4 - 10

Streptomyces sp. TN119

C6FX34

purified recombinant enzyme, 1 h, 37°C, 85% remaining activity

707196

4 - 10

Thermobifida alba

-

1 h, stable

171636

4 - 10

Thermothelomyces thermophilus

-

-

742216

4 - 11

Acetivibrio thermocellus

-

completely stable at pH 7.0, over 50% activity remaining at pH 4.0-11.0, inactivation at pH 12.0 and pH below 2.0

751347

4 - 11

Bacillus subtilis

Q45VU2

near constant transxylosylation activity

696821

4 - 11

Streptomyces ipomoeae

MK331807

stable

750086

4 - 11

Trichoderma inhamatum

-

-

695668

4 - 12

Bacillus subtilis

-

18h, 4°C, stable

751229

4 - 12

Streptomyces sp. S27

-

XynBS27 shows good pH stability and retains more than 80% of the maximum activity after incubation at 37°C for 1 h. Between 5-8 retains over 65% of its activity

695696

4 - 7

Neocallimastix patriciarum

A8TGA1

when pH is below 4.0 or above 7.0, less than 60% of the optimal activity is retained and the enzyme shows no activity at pH 9.0

695791

4 - 7

Thermotoga petrophila

A5IL00

the enzyme retains residual enzymatic activity of greater than 50% at pH 4.0-7.0

748630

4 - 8

Rhizophlyctis rosea

A0A2U8LMQ3

stable

749718

4 - 8

Trametes hirsuta

-

40°C, 10 min, stable

171629

4 - 9

Aspergillus fumigatus

-

-

742216

4 - 9

uncultured microorganism

E2DQY5

purified recombinant RuCelA, 24 h, 80% activity remaining

717134

4 - 9

uncultured microorganism

E2DQY5

xylan as substrate, remains more than 80% of its activity

717134

4 - 9.5

Aspergillus flavus

B8NJ86

purified native, over 80% activity remaining

750988

4 - 9.5

Streptomyces cyaneus

-

at 60°C for 1 h, retaining 80% of its activity

678757

4.5

Penicillium griseofulvum

-

purified recombinant PgXynB is stable at 30 °C, pH 4.5 for 240 min, with more than 85% of the activity remaining

707567

4.5

Pseudopedobacter saltans

F0S6Y3

purified recombinant enzyme, 25°C, 90 min, 25% activity remaining

752084

4.5 - 10.5

Bacillus sp. (in: Bacteria)

-

45°C, 1 h, enzyme form strain W1, W2, W3 and W4, stable

171621

4.5 - 10.5

Streptomyces sp.

-

stable

171660

4.5 - 7

Cellulosimicrobium sp.

-

retains over 80% of its original activity in the range pH 4.5-7.0

677613

4.5 - 7

Trichoderma virens

-

40°C, 60 min, stable

171632

4.5 - 9

Cutaneotrichosporon cutaneum

-

30°C, stable for at least 1 h

171625

4.5 - 9

Penicillium sp. CGMCC 1669

D1GFE6

purified recombinant enzyme, stable

707207

5

Aspergillus cf. niger BCC14405

Q6QA21

stable above

665441

5

Aspergillus niger

-

60°C, xylanase I, most stable at, half-life: 20 min

171597

5

Pediococcus acidilactici

-

purified extracellular enzyme, 85% activity remaining after 24 h, 70% activity remaining after 168 h

750814

5

Schizophyllum commune

P35809

18 h, less than 30% of initial activity

729654

5

Thermomyces lanuginosus

-

stable up to

171643

5

Thermomyces lanuginosus

-

50°C, 51 h, 60% residual activity

742958

5

Thermothelomyces heterothallicus

-

50°C, 24 h, 80% remaining activity

655176

5 - 10

Aspergillus ochraceus

-

45°C, 30 min, stable

171654

5 - 10

Halalkalibacterium halodurans

-

-

681342

5 - 10

Irpex lacteus

-

30°C, 24 h, xylanase I is stable

171612

5 - 10

Melanocarpus albomyces

-

50°C, 24 h, stable, xylanase IA and xylanase IIIA

738730

5 - 10

Melanocarpus albomyces

-

50°C, 24, stable

738730

5 - 10

Melanocarpus albomyces

-

50°C, stable for 24 h, both isoforms xylanase IA and xylanase IIIA

742924

5 - 10

Microbacterium trichothecenolyticum

A0A067XRW0

-

730191

5 - 10

Thermomonospora sp.

-

stable, 75% residual activity

654413

5 - 11

Actinomadura sp.

-

purified recombinant XynS14s, stable

717886

5 - 11

Nesterenkonia xinjiangensis

D1KJJ7

purified recombinant enzyme, the enzyme retains more than 80% of initial activity after 1 h at pH 5.0-11.0 at 37 °C for 1 h

707216

5 - 11

Ruminiclostridium josui

-

4°C, RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide is stable for 16 h

750435

5 - 11

Termitomyces aurantiacus

-

-

742400

5 - 11

Thermomyces lanuginosus

-

-

742400

5 - 12

Thermoclostridium stercorarium

-

4°C, 24 h, stable

171661

5 - 12

Thermomyces lanuginosus

-

-

742131

5 - 12

Thermomyces lanuginosus

-

stable

171643

5 - 6

Hordeum vulgare

-

most stable at

171605

5 - 6

Trichoderma longibrachiatum

-

40°C, 8-10 h, 25% loss of activity

171647

5 - 7

Aspergillus niger

-

purified native enzyme, 50°C, 86% residual activity after 45 min, the crude enzyme retains only 6% of the initial activity

710686

5 - 7

Aspergillus niger

I3QKR9

1 h, 40°C, wild-type enzyme is stable

751243

5 - 7

Irpex lacteus

-

30°C, 24 h, xylanase II is stable

171612

5 - 7

Schizophyllum commune

-

stable

664785

5 - 7.5

Euphausia superba

-

isozymes A and B

655257

5 - 7.5

Fusarium proliferatum

-

fully stable, 1 h at 40°C, and 30 min at 55°C

657201

5 - 8

Acidothermus cellulolyticus

A0LR95

purified recombinant enzyme, stable at

750497

5 - 8

Acidothermus cellulolyticus

A0LR95

the enzyme remains completely stable after 30 min incubation between pH 5.0 and 8.0

750497

5 - 8

Paenibacillus curdlanolyticus

B1A3N2

still 80% stability at pH 9.0

699498

5 - 8

Streptomyces cyaneus

-

retains more than 60% of its activity

678757

5 - 8

Thermomyces lanuginosus

-

stable over the range

171617

5 - 9

Bacillus licheniformis

-

over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C

682685

5 - 9

Bacillus sp. (in: Bacteria)

-

purified native enzyme, stable

708851

5 - 9

Thermomyces lanuginosus

-

stable

742227

5.5

Hordeum vulgare

-

optimal stability

171605

5.5

Pseudopedobacter saltans

F0S6Y3

purified recombinant enzyme, 25°C, 90 min, 50% activity remaining

752084

5.5

Thermoclostridium stercorarium

-

4°C, 24 h, xylanase A loses 50% of its activity

171615

5.5

Thermopolyspora flexuosa

-

recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 37 min with substrate, 19 min without substrate, isolated catalytic domain: life is 17 min with substrate, 33 min without substrate

707229

6

Aspergillus niger

-

60°C, xylanase II, most stable at, half-life: 75 min

171597

6

Thermothelomyces heterothallicus

-

55% remaining activity

655176

6

Thermotoga petrophila

A5IL00

stable at pH 6.0 (citrate phosphate buffer)

748630

6 - 10

Aspergillus terreus

-

90 min, the enzyme retains 75-85% of its initial activity

750795

6 - 10

Bacillus sp. (in: Bacteria)

-

purified enzyme, stable

717105

6 - 10

Paenibacillus sp.

A0A075EEX4

-

730187

6 - 10

Ruminiclostridium josui

-

4°C, enzyme derivative RjAbf62A-CBM6, containing the glycoside hydrolase family 62 (GH62) catalytic module and the family 6 carbohydrate binding module (CBM6)retains its initial activity after a 16 h incubation

750435

6 - 10

Thermochaetoides thermophila

Q6UN40

recombinant enzyme

701091

6 - 8

Pseudopedobacter saltans

F0S6Y3

purified recombinant enzyme, 25°C, 90 min, over 80% activity remaining

752084

6 - 8

Schizophyllum commune

-

stable

171590

6 - 8

Talaromyces purpureogenus

A0A2H4RAC5

purified recombinant enzyme, 37°C, 24 h, 80% activity remaining

749635

6 - 9

Halalkalibacterium halodurans

-

-

681342

6 - 9

Humicola insolens

-

15 h, xylanase 2 is stable

738127

6 - 9

Pseudoalteromonas haloplanktis

Q8RJN8

-

656075

6 - 9

Thermomyces lanuginosus

-

stable

737547

6.5

Bacillus pumilus

-

stability optimum

171586

6.5

Termitomyces clypeatus

-

purified enzyme, most stable at

707210

6.5

Thermomyces lanuginosus

-

50°C, 51 h, 100% residual activity

742958

7

Aspergillus niger

-

purified native enzyme, 40°C, 120 min, 80% activity remaining

710686

7

Bacillus sp. (in: Bacteria)

Q84F19

3 h, stable

664690

7

Geobacillus stearothermophilus

-

65°C, stable for more than 10 h

171651

7

Pediococcus acidilactici

-

purified extracellular enzyme, 95% activity remaining after 24 h, 80% activity remaining after 168 h

750814

7

Thermopolyspora flexuosa

-

recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 37 min with substrate, 23 min without substrate, isolated catalytic domain: life is 17 min with substrate, 22 min without substrate

707229

7 - 8

Bacillus sp. (in: Bacteria)

-

purified enzyme, 4 h, 80% activity remaining

717091

7 - 8

Thermomyces dupontii

-

50°C, stable for 7 days

741630

7.5

Penicillium griseofulvum

-

around 30% activity is still observed at pH 7.5

677745

7.5

Talaromyces funiculosus

Q9HFH0

no activity is observed at pH 7.5

677745

8

Aspergillus terreus

-

90 min, enzyme retains 95.12% of its initial activity

750795

8

Bispora sp. MEY-1

D6MYS9

and above, 60 min, complete inactivation

707258

8

Cellulosimicrobium sp.

-

significantly inactivated at pHs over 8.0

677613

8

Humicola insolens

-

15 h, xylanase 1 loses 46% of its activity

738127

8

Saccharolobus solfataricus

-

retains 63% activity at pH 8.0

679721

8

Thermomyces lanuginosus

-

50°C, 51 h, 90% residual activity

742958

8.5

Pseudopedobacter saltans

F0S6Y3

purified recombinant enzyme, 25°C, 90 min, 50% activity remaining

752084

8.5

Thermopolyspora flexuosa

-

recombinant His-tagged enzyme, 30 min, 80°C, wild-type enzyme: half-life is 33 min with substrate, 23 min without substrate, isolated catalytic domain: life is 14 min with substrate, 26 min without substrate

707229

9

Aspergillus fumigatus

-

1 h, 37°C, 70% residual activity

737935

9

Bacillus amyloliquefaciens

-

50°C, 5 h, stable

171646

9

Geobacillus stearothermophilus

-

half-life: 6 h

171651

9

Halalkalibacterium halodurans

-

the enzyme shows good thermal stability when incubated at 65°C in pH 9 buffer

679547

9

Humicola insolens

-

15 h, xylanase 1 loses 15% of its activity

738127

9

Pediococcus acidilactici

-

purified extracellular enzyme, 90% activity remaining after 24 h, 80% activity remaining after 168 h

750814

9

Pseudopedobacter saltans

F0S6Y3

purified recombinant enzyme, 25°C, 90 min, 25% activity remaining

752084

9

Saccharolobus solfataricus

-

retains 40% activity at pH 9.0

679721

9

Trichoderma longibrachiatum

-

stable above

171647

additional information

Bacillus subtilis

P18429

molecular mechanisms governing the pH-dependent stability of GH11 proteins, overview

710466

additional information

Bacillus subtilis

-

molecular mechanisms governing the pH-dependent stability of GH11 proteins, overview

710466