3.2.1.8: endo-1,4-beta-xylanase This is an abbreviated version!go to the full flat file .
Reaction
(Xylbeta(1-4))n H2O (Xylbeta(1-4))n-m (Xylbeta(1-4))m
Synonyms (1--> 4)-beta-xylan 4-xylanohydrolase, (1-4)-beta-xylan 4-xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase 22, 1,4-beta-D-xylan-xylanohydrolase, 1,4-beta-xylan xylanohydrolase, 1,4-beta-xylanase, 34 kDa xylanase, Abf51A, Abf62A-Axe6A, Acel_0180, acidophilic endo-1,4-beta-xylanase, AfXynA, AfXynB, alkaline active xylanase, alkaline xylanase, AMX-4 xylanase, AnxB, AxB8, Axy43A, basic xylanase, Bcx, beta-1, 4-endoxylanase, beta-1,4-D-xylanase, beta-1,4-endoxylanase, beta-1,4-xylan hydrolase, beta-1,4-xylan xylanohydrolase, beta-1,4-xylanase, beta-1,4endoxylanase, beta-D-xylanase, beta-endoxylanase, beta-xylanase, bifunctional cellulase/xylanase, bifunctional endoglucanase/xylanase, bifunctional xylanase/endoglucanase, BlxA, BSX, BSXY, Btx, Calow_0124, ctendo7, Cthe_3012, CTHT_0045780, CtXynGH30, EGXA, endo(1-4)beta-xylanase, endo-(1,4)-beta-xylanase, endo-(1--> 4)-beta-xylanase, endo-1,4-beta-D-xylanase, endo-1,4-beta-xylanase, endo-1,4-beta-xylanase II, endo-1,4-xylanase, endo-acting beta-1,4-xylanase, endo-beta-(1'4)-xylanase, endo-beta-(1,4)-xylanase, endo-beta-1, 4-xylanase, endo-beta-1,4-xylanase, endo-beta-1,4-xylanase 2, endo-beta-1,4-xylanase2, endo-xylanase, endoxylanase, endoxylanase I, endoxylanase NtSymX11, endoxylanase Xys1DELTA, EXY1, family 11 endoxylanase, family 11 xylanase, family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase, family-10 endo-1,4-beta-xylanase, FIA-xylanase, FOTG_15646, G/11 endo-1,4-beta-xylanase, GC25 xylanase, GH 10 xylanase, GH 11 xylanase, GH-10 endo-1,4-beta-xylanase, GH-11 endo-1,4-beta-xylanase, GH10 endo-beta-1,4-xylanase, GH10 xylanase D, GH11 xylanase, GH43B6, GH7 endoglucanase, GHF 10 endoxylanase, GHF 11 endoxylanase, glycoside hydrolase family 11 endoxylanase, glycoside hydrolase family 8 domain protein, GXYN, KRICT PX1, MalAC0309_0409, More, MROS_2090, MROS_2091, MROS_2495, Mxyn10, MYCTH_49824, MYCTH_56237, ORF4, Pedsa_2704, PhX20, PhX33, PsGH10A, pXyl, RrXyn11A, RuCelA, Rut C-30, SCO5931, SipoEnXyn10A, SlxB, SoxB, SSO1354 protein, TAXI, TERTU_4506, Tfu_1213, TLX, TmxB, Tpet_0854, TRX II, TtGH8, Wxl1, X-I, X-II, X34, Xa, Xln-1, xlnA, xlnB, XT6, Xyl, Xyl I, Xyl II, XYL1, Xyl10A, Xyl10B, XYL10C, Xyl11, XYL1p, XYL2, Xyl2090, Xyl2091, Xyl2495, Xyl30, XYLA, xylanase, xylanase 1, xylanase 10A, xylanase 10B, xylanase 10C, xylanase 11 A, xylanase 11A, xylanase 11J, xylanase 2, Xylanase 22, xylanase 43A, xylanase A, xylanase B, xylanase bI, xylanase bII, xylanase C, xylanase I, xylanase II, xylanase III, xylanase J, xylanase LC9, xylanase Xyl10A, xylanase Xyl11A, xylanase XynZF-2, xylanase, endo-1,4-, XylB, XylB8, XylC, XYLD, XylE, XylF2, XylG, xylH, xylM, XylX, XYLY, Xyn II, Xyn III, Xyn-b39, Xyn-Lxy, Xyn1, Xyn10, Xyn10A, Xyn10B, Xyn10E, XYN10G5, XYN10Ks_480, Xyn11, Xyn11A, XYN11F63, XYN11Ks_480, Xyn11NX, Xyn12.2, Xyn162, Xyn2, Xyn3, Xyn30A, Xyn5, XynA, XynA119, XynA19, XynA4, XynAS27, XynAS9, XynB, XynB18, XynBS27, XynC, XynD, XynE15, XynE2, XynG1, XynGH30, XynGR40, XYNII, XynIII, XynJ, XynS14, XynS20, XynSW1, XynSW3, XynT, XynY, XynZ, Xys1, Xys1delta, xysA
ECTree
Purification
Purification on EC 3.2.1.8 - endo-1,4-beta-xylanase
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13.3fold from culture filtrate to homogeneity by ion exchange and gel filtration chromatography
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13.3fold to homogeneity
Thermomonospora sp.
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3 enzymes to homogeneity from culture supernatant
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33.5fold to homogeneity by ultrafiltration, cation exchange chromatography, and gel filtration
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38.7fold purified by gel filtration, to homogeneity
44871fold from ripe fruit mesocarp, to homogeneity
7 isozymes to homogeneity by gel filtration and ion-exchange chromatography, overview
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975fold from the culture supernatant, to homogeneity by ammonium sulfate precipitation and several chromatographic steps
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a 20000 Da enzyme form and a 29000 Da enzyme form
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acidic endo-beta-1,4-xylanase
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affinity-purified by formation of artificial oil bodies
after expression in Aspergillus niger
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ammonium sulfate precipitation and DEAE-Sepharose column chromatography
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ammonium sulfate precipitation and Sephadex G-100 gel filtration
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ammonium sulfate saturation, CM-Sepharose FF column chromatography, and Sephacryl S-200 HR gel filtration
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by a single step of affinity purification on avicel, 139.4fold with a 38.1% yield
by affinity chromatography
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by ammonium sulfate fractionation, centrifugation and gel filtration, 7.7fold
by ammonium sulfate precipitation and hydrophobic interaction chromatography, to homogeneity, 2 isozymes
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by ammonium sulfate precipitation, anion-exchange and hydrophobic interaction column chromatographies, 17.5fold purified
by gel filtration to apparent homogeneity, 39fold with 11% yield
by gel filtration to homogeneity with a purification yield of 11.3%
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by microbial DNA purification kit
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centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4°C, purity monitored by SDS-PAGE and isoelectric focusing
DEAE Sepharose Fast Flow column chromatography and Sephadex G-75 gel filtration
Halalkalibacterium halodurans
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DEAE-Toyopearl 650 M column chromatography, 16/10 DEAE fast-flow column chromatography, and Mono-Q HR 5/5 column chromatography
Halalkalibacterium halodurans
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enterokinase-treated enzyme purified by a combination of ammonium sulfate precipitation, gel filtration and ion exchange chromatography, to homogeneity
Thermochaetoides thermophila
enzyme form X-1, X-b-1 and X-b-II
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enzyme XYLY, from recombinant Escherichia coli
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ethanol precipitation, Sephadex G-75 gel filtration, CM Sephadex C-50 gel filtration, Mono Q HR 5/5 column chromatography, and Q-Sepharose column chromatography
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expressed in Pichia pastoris, His-tagged protein
from 4 strains, to homogeneity
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from bagasse-grown cultures
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from culture supernatant, 22.12fold to homogeneity by ultrafiltration and a combination of ion exchange and gel filtration chromatography
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from culture supernatant, to homogeneity
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full-length enzyme and its variants
fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
heat treatment purification
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heavier protein band (42.8 kDa) partially purified
HiPrep 16/10 DEAE FF chromatography, HiPrep 16/10 CM FF column chromatography, and Mono Q resin column chromatography
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His Trap HP column chromatography and HiPrep 26/60 Sephacryl S-100 gel filtration
HisTrap column chromatography and Mono-Q column chromatography
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inexpensive, rapid procedure for bulk purification
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intact, CBM-truncated and CBM-linker-truncated versions of the mature proteins purified to electrophoretic homogeneity, by ion exchange chromatography or gel filtration
isoforms xylanase I, xylanase II
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isozymes A, 118fold, and B, 82fold
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isozymes XA-1 and XA-2 from culture medium, to homogeneity, purification of Xa-1 56.1fold, of Xa-2 91.2fold
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Mono Q column chromatography and Superdex 75 gel filtration
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mutant purified by immobilized metal-ion affinity chromatography and gel-filtration
mutants and full-length protein Xyn11 purified by cation exchange chromatography and gel filtration, to more than 95% electrophoretic purity
mutants purified by one-step affinity purification
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native enzyme 16.6fold by ammonium sulfate fractionation and gel filtration
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native enzyme 28.8fold by ammonium sulfate fractionation and gel filtration to homogeneity
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native enzyme 29.1fold to homogeneity by ammonium sulfate fractionation, gel filtration and anion exchange chromatography
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native enzyme 36.7fold by anion exchange chromatography, gel filtration, and ultrafiltration, and a second step of gel filtration
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native enzyme 4fold by consecutive ultrafiltration and anion exchange chromatography
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native enzyme 678.1fold from strain YJ6 to homogeneity by ammonium sulfate fractionation, dialysis, cation exchange chromatography, ultrafiltration, and gel filtration
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native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
native enzymes by anion exchange chromatography and gel filtration, PhX33 3.29fold, PhX20 5.73fold
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native extracellular 5.6fold Xyn3 to homogeneity by anion exchange chromatography, concentration by PEG, and gel filtration
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native extracellular enzyme 167fold to homogeneity
native extracellular enzyme 25.7fold to homogeneity by anion exchange chromatography and gel filtration
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native extracellular enzyme 4.6fold by acetone precipitation, anion exchange and cation exchange chromatography
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native extracellular enzyme from crude culture supernatant 1.7fold by ammonium sulfate fractionation, dialysis, and gel filtration, to homogeneity
native extracellular enzyme from culture supernatant 3.7fold by ammonium sulfate fractionation and gel filtration
native extracellular xylanase partially by ammonium sulfate fractionation and dialysis
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native isozymes EX1 and EX2 are purified from crude extract of the strain K9301 in solid fermentation by anion exchange chromatography and gel filtration to homogeneity
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native wild-type, 17fold, and mutant enzymes, to homogeneity by anion exchange chromatography and gel filtration
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native xylanase I and xylanase II 2.4-2.5fold by anion exchange chromatography and gel filtration to homogeneity
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Ni-NTA spin column chromatography
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partial, 21fold by three-phase partitioning of urea-DTT-denatured enzyme
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partially purified by ammonium sulphate fractionation
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Phenyl Sepharose chromatography and Superdex-200 HR Superdex-200 gel filtration
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Q10 anion exchange column chromatography
recombinant enzyme domains from Escherichia coli
recombinant enzyme expressed in Escherichia coli
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recombinant enzyme from Escherichia coli
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recombinant enzyme from Escherichia coli by heat treatment at 65°C for 15 min and hydroxyapatite chromatography
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recombinant enzyme from Escherichia coli strain BL21 (DE3)
recombinant enzyme from Escherichia coli, native enzyme 11fold by ultrafiltration and ion exchange chromatography
recombinant enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and gel filtration
recombinant enzyme from Pichia pastoris, and native enzyme, by gel filtration
recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration
recombinant enzyme with his-tag
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recombinant enzyme Xyn10B 10fold from Escherichia coli strain BL21 CodonPlus (RIPL) to homogeneity by adsorption chromatography on amorphous cellulose resin, dialysis, and ultrafiltration
recombinant enzyme, overexpressed in Escherichia coli
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recombinant extracellular C-terminally His-tagged Xys1 from Streptomyces lividans
recombinant extracellular enzyme 185fold, the enzyme is deglycosylated by EndoH, followed by ultrafiltration, and anion exchange chromatography
recombinant extracellular enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and again ultrafiltration
recombinant extracellular His- and c-myc-tagged enzyme from Pichia pastoris strain GS115 by anion exchange and cation exchange chromatography
recombinant GST- or His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), the His-tagged enzymes by nickel affinity chromatography, the GST-tagged by glutathione affinity chromatography and cleavage of the tag by thrombin. The specific activity of thrombin-cleaved PgXynB is 5fold higher than His6-tagged PgXynB
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recombinant His-tagged enzyme 48fold from Escherichia coli by nickel affinity chromatography
recombinant His-tagged enzyme from Esccherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) culture supernatant to homogeneity by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homnogeneity
recombinant His-tagged enzyme from Escherichia coli strain BVL21 by affinity chromatography
recombinant His-tagged enzyme from Escherichia coli, 12.5fold to homogeneity by Ni2+-chelate affinity chromatography
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recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography
Thermochaetoides thermophila
recombinant His-tagged enzyme from Pichia pastoris strain X33 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged Xyn2 1.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
B2CNY5
recombinant His-tagged XynA from Escherichia coli by nickel affinity and anion exchange chromatography
recombinant His-tagged XynA19 from Escherichia coli strains Top10 and BL21(DE3) by ultrafiltration and nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by 3C protease, another step of nickel affinity chromatography, and gel filtration
recombinant TfxA and the mutants purified to homogeneity on nickel-chelating column
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recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(lambdaDE3) by cation exchange chromatography and gel filtration
recombinant wild-type and mutant enzymes from Pichia pastoris strain X-33 by TAXI inhibitor affinity chromatography
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recombinant Xyl10C 1.1fold from Pichia pastoris by anion exchange chromatography
recombinant XYL1p from Pichia astoris by anion exchange chromatography
recombinant xylanase A produced in Escherichia coli
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recombinant Xyn2 from Pichia pastoris strain PX-1
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recombinant XynBS27 purified to electrophoretic homogeneity by anion exchange chromatography
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recombinant XynD 1.9fold from Pichia pastoris strain X33 by ultrafiltration and gel filtration
recombinant XynS14 from Pichia pastoris by ultrafiltration and gel filtration, recombinant His-tagged XynS14 from Escherichia coli by heat treatment, nickel affinity chromatography, and gel filtration
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recombinantz enzyme 2.33fold from Pichia pastoris to homogeneity from culture medium
shaking of wheat kernels with acetate buffer (25 mM, pH 5.0, 0.02% sodium azide) for 17 h, liquid phase dialyzed against 10 mM sodium acetate buffer, pH 5.0 at 7°C for 24 h, dialysate concentrated by freeze-drying and resuspension in water
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Superdex 200 HR 10/30 gel filtration and Mono Q HR 5/5 column chromatography
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the chimeric enzymes, CorA-XynA and CorA-XynAG3, the parental enzyme XynA, and the thermostable variant XynAG3 are produced in Escherichia coli
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the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin
three consecutive purification steps, gel filtration, 96% purity
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to apparent homogeneity by gel filtration
to electrophoretic homogeneity by ammonium sulfate precipitation, ion exchange chromatography, and hydrophobic interaction chromatography, 7.1fold purified, with a final activity yield of 18.1%
to homogeneity by acetone precipitation and anion-exchange chromatography, 6.7fold with a final activity yield of 12.7%
to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration
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to homogeneity by gel filtration and ion exchange chromatography
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two enzyme form: Xys1S and Xys1L
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two xylanase activities, a minor xylanase IA and a major xylanase IIIA, are purified
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two xylanase activities, a minor xylanase IA and a major xylanase IIIA, were purified to apparent homogeneity from bagasse-grown cultures
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using Ni-nitrilotriacetic acid magnetic agarose beads
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using Ni-NTA agarose beads
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wild-type, 27.5fold, and mutant strain enzyme, 30.6fold, to homogeneity from culture supernatant
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xylanase A and xylanase B
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xylanase X-I, X-II-A and X-II-B
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xylanases: Ia, Ib, IIA, IIB, IIC and IID
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Halalkalibacterium halodurans
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Thermochaetoides thermophila
partial
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recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration
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recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration
recombinant protein
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xylanase I and II
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3.2.1.8 endo-1,4-beta-xylanase
results (
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