3.2.1.8: endo-1,4-beta-xylanase This is an abbreviated version! For detailed information about endo-1,4-beta-xylanase, go to the full flat file .
Reaction
(Xylbeta(1-4))n +
H2O =
(Xylbeta(1-4))n-m +
(Xylbeta(1-4))m
Synonyms (1--> 4)-beta-xylan 4-xylanohydrolase, (1-4)-beta-xylan 4-xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase 22, 1,4-beta-D-xylan-xylanohydrolase, 1,4-beta-xylan xylanohydrolase, 1,4-beta-xylanase, 34 kDa xylanase, Abf51A, Abf62A-Axe6A, Acel_0180, acidophilic endo-1,4-beta-xylanase, AfXynA, AfXynB, alkaline active xylanase, alkaline xylanase, AMX-4 xylanase, AnxB, AxB8, Axy43A, basic xylanase, Bcx, beta-1, 4-endoxylanase, beta-1,4-D-xylanase, beta-1,4-endoxylanase, beta-1,4-xylan hydrolase, beta-1,4-xylan xylanohydrolase, beta-1,4-xylanase, beta-1,4endoxylanase, beta-D-xylanase, beta-endoxylanase, beta-xylanase, bifunctional cellulase/xylanase, bifunctional endoglucanase/xylanase, bifunctional xylanase/endoglucanase, BlxA, BSX, BSXY, Btx, Calow_0124, ctendo7, Cthe_3012, CTHT_0045780, CtXynGH30, EGXA, endo(1-4)beta-xylanase, endo-(1,4)-beta-xylanase, endo-(1--> 4)-beta-xylanase, endo-1,4-beta-D-xylanase, endo-1,4-beta-xylanase, endo-1,4-beta-xylanase II, endo-1,4-xylanase, endo-acting beta-1,4-xylanase, endo-beta-(1'4)-xylanase, endo-beta-(1,4)-xylanase, endo-beta-1, 4-xylanase, endo-beta-1,4-xylanase, endo-beta-1,4-xylanase 2, endo-beta-1,4-xylanase2, endo-xylanase, endoxylanase, endoxylanase I, endoxylanase NtSymX11, endoxylanase Xys1DELTA, EXY1, family 11 endoxylanase, family 11 xylanase, family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase, family-10 endo-1,4-beta-xylanase, FIA-xylanase, FOTG_15646, G/11 endo-1,4-beta-xylanase, GC25 xylanase, GH 10 xylanase, GH 11 xylanase, GH-10 endo-1,4-beta-xylanase, GH-11 endo-1,4-beta-xylanase, GH10 endo-beta-1,4-xylanase, GH10 xylanase D, GH11 xylanase, GH43B6, GH7 endoglucanase, GHF 10 endoxylanase, GHF 11 endoxylanase, glycoside hydrolase family 11 endoxylanase, glycoside hydrolase family 8 domain protein, GXYN, KRICT PX1, MalAC0309_0409, More, MROS_2090, MROS_2091, MROS_2495, Mxyn10, MYCTH_49824, MYCTH_56237, ORF4, Pedsa_2704, PhX20, PhX33, PsGH10A, pXyl, RrXyn11A, RuCelA, Rut C-30, SCO5931, SipoEnXyn10A, SlxB, SoxB, SSO1354 protein, TAXI, TERTU_4506, Tfu_1213, TLX, TmxB, Tpet_0854, TRX II, TtGH8, Wxl1, X-I, X-II, X34, Xa, Xln-1, xlnA, xlnB, XT6, Xyl, Xyl I, Xyl II, XYL1, Xyl10A, Xyl10B, XYL10C, Xyl11, XYL1p, XYL2, Xyl2090, Xyl2091, Xyl2495, Xyl30, XYLA, xylanase, xylanase 1, xylanase 10A, xylanase 10B, xylanase 10C, xylanase 11 A, xylanase 11A, xylanase 11J, xylanase 2, Xylanase 22, xylanase 43A, xylanase A, xylanase B, xylanase bI, xylanase bII, xylanase C, xylanase I, xylanase II, xylanase III, xylanase J, xylanase LC9, xylanase Xyl10A, xylanase Xyl11A, xylanase XynZF-2, xylanase, endo-1,4-, XylB, XylB8, XylC, XYLD, XylE, XylF2, XylG, xylH, xylM, XylX, XYLY, Xyn II, Xyn III, Xyn-b39, Xyn-Lxy, Xyn1, Xyn10, Xyn10A, Xyn10B, Xyn10E, XYN10G5, XYN10Ks_480, Xyn11, Xyn11A, XYN11F63, XYN11Ks_480, Xyn11NX, Xyn12.2, Xyn162, Xyn2, Xyn3, Xyn30A, Xyn5, XynA, XynA119, XynA19, XynA4, XynAS27, XynAS9, XynB, XynB18, XynBS27, XynC, XynD, XynE15, XynE2, XynG1, XynGH30, XynGR40, XYNII, XynIII, XynJ, XynS14, XynS20, XynSW1, XynSW3, XynT, XynY, XynZ, Xys1, Xys1delta, xysA
ECTree
Activating Compound
Activating Compound on EC 3.2.1.8 - endo-1,4-beta-xylanase
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(NH4)2SO4
-
xylanase activity is 9.7 U/ml with 0.1% urea
2,4-dithiothreitol
-
stimulation, both xylanase I and xylanase II
acetone
5%, 4.3fold activation of activity with carboxymethyl cellulose, slight activation of xylanase activity (to 104.6% of control), fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
alpha-cyclodextrin
-
11% increased activity at 1 mM
bagasse pulp
-
Thermomyces lanuginosus SSBP produces high level of cellulase-free xylanase during growth on a cheap bagasse pulp carbon source after 120 h
-
beta-cyclodextrin
-
9% increased activity at 1 mM
butanol
5%, 1.1fold activation of activity with carboxymethyl cellulose, 73% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
casein hydrolysate
-
xylanase activity is 5.3 U/ml with 0.1% urea
-
Cr3+
10 mM, activity increases by 28.2%
CTAB
-
activity is slightly enhanced
Cu2+
stimulates activity at 5 mM by 20%
cyanamide
-
3% increased activity at 1 mM
glucose
-
highest specific activity of xylanase in supernatant fraction is observed in glucose culture
glycerol
5%, 8.2fold activation of activity with carboxymethyl cellulose, 10% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
iodoacetic acid
-
activation of isozymes Ic and IIB
Li+
stimulates activity with increasing amounts from 5 mM (25%) to 20 mM (38%)
maltose
-
stimulates xylanase A
NaNO3
-
xylanase activity is 11.9 U/ml with 0.1% urea
NH4Cl
-
xylanase activity is 10.5 U/ml with 0.1% urea
Pb(CH3COO)2
-
activates xylanase II and inhibits xylanase I at 10 mM
PMSF
-
activates xylanase I, inhibits xylanase II
reduced glutathione
-
3% increased activity at 1 mM
Sodium citrate
-
activates
trehalose
-
activates both isozymes and stabilizes them at higher temperatures
tris-(2-carboxyethyl)-phosphine
activates
tryptone
-
xylanase activity is 6.5 U/ml with 0.1% urea
-
yeast extract
-
xylanase activity is 6.2 U/ml with 0.1% urea
-
2-mercaptoethanol
-
-
2-mercaptoethanol
activates at 1 mM
2-mercaptoethanol
-
isoform xyl I, 59% activation, xyl II, 102% activation
2-mercaptoethanol
-
native enzyme
2-mercaptoethanol
0.5%, 155% of initial activity
2-mercaptoethanol
activates 16% at 10 mM
2-mercaptoethanol
Halalkalibacterium halodurans
-
2-mercaptoethanol
activates 1.9fold at 10 mM
2-mercaptoethanol
activates
2-mercaptoethanol
activates by 35% at 10 mM
2-mercaptoethanol
-
31% increased activity at 1 mM
2-mercaptoethanol
5 mM, 152% of initial activity
2-mercaptoethanol
activates 1.6fold at 10 mM
2-mercaptoethanol
-
5 mM, 140% of initial activity
beta-mercaptoethanol
-
stimulates
beta-mercaptoethanol
-
10 mM, stimulates
beta-mercaptoethanol
-
52.66% increase of activity at 2 mM
beta-mercaptoethanol
10 mM enhances the activities of XynAS27, XynAS27cd, and XynAS27cdl by about 1.5, 1.7, and 1.4fold, respectively
beta-mercaptoethanol
-
enhances the activity about 1.3- and 1.6fold at the concentrations of 1 and 10 mM, respectively
beta-mercaptoethanol
10 mM enhances the activity about 1.6fold
Ca2+
-
slightly stimulates
Ca2+
stimulates activity with increasing amounts from 5 mM (16%) to 20 mM (31%)
Ca2+
10 mM, activity increases by 26.4%
Co2+
-
slightly stimulates
Co2+
stimulates activity from 5 mM (16%) to 10 mM (26%)
dithiothreitol
-
dithiothreitol
-
stimulation
dithiothreitol
-
stimulates
dithiothreitol
-
stimulates
dithiothreitol
0.5%, 240% of initial activity
dithiothreitol
Halalkalibacterium halodurans
-
dithiothreitol
-
11.6% increase of activity at 2 mM
dithiothreitol
-
6% increased activity at 1 mM
dithiothreitol
-
stimulates
dithiothreitol
-
5 mM, 113% of initial activity
DMSO
-
6.58% increase of activity at 2 mM
DTT
-
-
DTT
23% activation at 5 mM
EDTA
-
-
EDTA
-
1-5 mM, stimulates
EDTA
Halalkalibacterium halodurans
-
9% increase of activity at 1 mM
EDTA
Halalkalibacterium halodurans
-
1 mM, 114% of initial activity
EDTA
-
14.48% increase of activity at 2 mM
EDTA
-
5 mM, 118% of initial activity
EDTA
5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
glutathione
-
10 mM, 10% stimulation
glutathione
-
native enzyme
L-cysteine
-
-
L-cysteine
-
9% increased activity at 1 mM
Mg2+
-
slightly stimulates
Mg2+
stimulates activity with increasing amounts from 5 mM (14%) to 20 mM (57%)
Mn2+
stimulates activity from 5 mM (27%) to 10 mM (40%)
Mn2+
1 mM, 115% of initial activity
SDS
slightly activating
SDS
1%, 5.7fold activation of activity with carboxymethyl cellulose, 4.5fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
Triton X-100
0.5%, about 120% of initial activity
Triton X-100
1%, activity increases by 29.43%. 4%, activity increases by 32.21%
Triton X-100
42% activation at 0.5%
Tween 20
-
-
Tween 20
1%, activity increases by 40.21%. 4%, activity increases by 66.36%
Tween 80
0.5%, 140% of initial activity
Tween 80
0.5%, about 120% of initial activity
Tween-80
-
activity is slightly enhanced
Tween-80
38% activation at 0.5%
Urea
-
maximum xylanase activity (14.2 U/ml) is evident with 0.1% urea
Zn2+
stimulates activity at 5 mM by 25%
Zn2+
10 mM, activity increases by 4.0%
additional information
activity of rtEGXA depends on the concentration of Cl-
-
additional information
-
activity of rtEGXA depends on the concentration of Cl-
-
additional information
-
maximum endoxylanase activity at 72 h of cultivation
-
additional information
-
maximum endoxylanase activity at 72 h of cultivation
-
additional information
-
maximum endoxylanase activity at 96 h of cultivation
-
additional information
-
xylanase activity is maximum at 96 h of cultivation in 3% corncob containing medium. It exhibits a maximum endoxylanase activity and high percentage of xylooligosaccharides in the birch wood xylan hydrolysate by 24 h of incubation
-
additional information
-
maximum endoxylanase activity at 72 h of cultivation
-
additional information
Fe2+ has little effect on enzyme activity from 5 mM to 10 mM
-
additional information
-
Fe2+ has little effect on enzyme activity from 5 mM to 10 mM
-
additional information
endoxylanase activity is positively correlated with ash and negatively correlated with starch content
-
additional information
-
is not induced by carbohydrate growth substrates
-
additional information
-
activity is not affected by L-cysteine and 1,1-dithioerythreitol
-
additional information
Halalkalibacterium halodurans
-
enzyme displays 100% activity in presence of 0-20% NaCl
-
additional information
in the insoluble fraction of cell lysate or embedding in artificial oil bodies, XynS20-intein M-oleosin does not show any enzymatic activity. After release from artificial oil bodies by inclusion of DTT to induce intein linker self-splicing, XynS20 reveals a xylanase activity band of about 36 kDa
-
additional information
-
in the insoluble fraction of cell lysate or embedding in artificial oil bodies, XynS20-intein M-oleosin does not show any enzymatic activity. After release from artificial oil bodies by inclusion of DTT to induce intein linker self-splicing, XynS20 reveals a xylanase activity band of about 36 kDa
-
additional information
Xyn10A is a modular xylanase comprising nine domains, i.e., three family 22 carbohydrate-binding modules (CBMs), a family 10 catalytic domain of glycosyl hydrolases (xylanase), a family 9 CBM, a glycine-rich region, and three surface layer homology (SLH) domains. The CBMs of Xyn10A play an important role in the hydrolysis of plant cell walls
-
additional information
-
Xyn10A is a modular xylanase comprising nine domains, i.e., three family 22 carbohydrate-binding modules (CBMs), a family 10 catalytic domain of glycosyl hydrolases (xylanase), a family 9 CBM, a glycine-rich region, and three surface layer homology (SLH) domains. The CBMs of Xyn10A play an important role in the hydrolysis of plant cell walls
-
additional information
-
maximum endoxylanase activity at 72 h of cultivation
-
additional information
-
expression level of XynBS27 increases with induction time
-
additional information
Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect
-
additional information
endoxylanase activity is positively correlated with ash and negatively correlated with starch content
-
additional information
Thermochaetoides thermophila
xylose induces, is the best carbon source. Lactose is the best inducer, sucrose induces, glucose produces low levels of xylanase
-
additional information
-
higher level of xylanase activity is obtained in solid-state cultures than in submerged cultures after 120 h with 10% inoculum
-
additional information
the enzyme activity is not affected by EDTA
-
additional information
the enzyme activity is not affected by EDTA
-
additional information
-
maximum endoxylanase activity at 72 h of cultivation
-
additional information
-
maxima intracellular and extracellular xylanase production in standing condition are obtained with 96 h or 4 days of cultivation at 28°C using xylan as carbon source. In shaking condition, maxima are observed only at 60 h or 2.5 days at 28°C. Induction by glucose, xylose, lactose, CMC, celobiose and avicel
-
additional information
-
maximum endoxylanase activity at 96 h of cultivation
-
additional information
-
endoxylanase activity is positively correlated with ash and negatively correlated with starch content, thus endoxylanase activity in flour is strongly influenced by the level of bran contamination. There is little if any endoxylanase activity during dough mixing
-
additional information
-
endoxylanase activity level varies enormously between different flour fractions and is strongly positively correlated with ash and negatively correlated with starch content
-