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3.2.1.8: endo-1,4-beta-xylanase

This is an abbreviated version!
For detailed information about endo-1,4-beta-xylanase, go to the full flat file.

Word Map on EC 3.2.1.8

Reaction

(Xylbeta(1-4))n
+
H2O
=
(Xylbeta(1-4))n-m
 +
(Xylbeta(1-4))m

Synonyms

(1--> 4)-beta-xylan 4-xylanohydrolase, (1-4)-beta-xylan 4-xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase, 1,4-beta-D-xylan xylanohydrolase 22, 1,4-beta-D-xylan-xylanohydrolase, 1,4-beta-xylan xylanohydrolase, 1,4-beta-xylanase, 34 kDa xylanase, Abf51A, Abf62A-Axe6A, Acel_0180, acidophilic endo-1,4-beta-xylanase, AfXynA, AfXynB, alkaline active xylanase, alkaline xylanase, AMX-4 xylanase, AnxB, AxB8, Axy43A, basic xylanase, Bcx, beta-1, 4-endoxylanase, beta-1,4-D-xylanase, beta-1,4-endoxylanase, beta-1,4-xylan hydrolase, beta-1,4-xylan xylanohydrolase, beta-1,4-xylanase, beta-1,4endoxylanase, beta-D-xylanase, beta-endoxylanase, beta-xylanase, bifunctional cellulase/xylanase, bifunctional endoglucanase/xylanase, bifunctional xylanase/endoglucanase, BlxA, BSX, BSXY, Btx, Calow_0124, ctendo7, Cthe_3012, CTHT_0045780, CtXynGH30, EGXA, endo(1-4)beta-xylanase, endo-(1,4)-beta-xylanase, endo-(1--> 4)-beta-xylanase, endo-1,4-beta-D-xylanase, endo-1,4-beta-xylanase, endo-1,4-beta-xylanase II, endo-1,4-xylanase, endo-acting beta-1,4-xylanase, endo-beta-(1'4)-xylanase, endo-beta-(1,4)-xylanase, endo-beta-1, 4-xylanase, endo-beta-1,4-xylanase, endo-beta-1,4-xylanase 2, endo-beta-1,4-xylanase2, endo-xylanase, endoxylanase, endoxylanase I, endoxylanase NtSymX11, endoxylanase Xys1DELTA, EXY1, family 11 endoxylanase, family 11 xylanase, family 30 glycoside hydrolase subfamily 8 glucuronoxylan endo-beta-1,4-xylanase, family-10 endo-1,4-beta-xylanase, FIA-xylanase, FOTG_15646, G/11 endo-1,4-beta-xylanase, GC25 xylanase, GH 10 xylanase, GH 11 xylanase, GH-10 endo-1,4-beta-xylanase, GH-11 endo-1,4-beta-xylanase, GH10 endo-beta-1,4-xylanase, GH10 xylanase D, GH11 xylanase, GH43B6, GH7 endoglucanase, GHF 10 endoxylanase, GHF 11 endoxylanase, glycoside hydrolase family 11 endoxylanase, glycoside hydrolase family 8 domain protein, GXYN, KRICT PX1, MalAC0309_0409, More, MROS_2090, MROS_2091, MROS_2495, Mxyn10, MYCTH_49824, MYCTH_56237, ORF4, Pedsa_2704, PhX20, PhX33, PsGH10A, pXyl, RrXyn11A, RuCelA, Rut C-30, SCO5931, SipoEnXyn10A, SlxB, SoxB, SSO1354 protein, TAXI, TERTU_4506, Tfu_1213, TLX, TmxB, Tpet_0854, TRX II, TtGH8, Wxl1, X-I, X-II, X34, Xa, Xln-1, xlnA, xlnB, XT6, Xyl, Xyl I, Xyl II, XYL1, Xyl10A, Xyl10B, XYL10C, Xyl11, XYL1p, XYL2, Xyl2090, Xyl2091, Xyl2495, Xyl30, XYLA, xylanase, xylanase 1, xylanase 10A, xylanase 10B, xylanase 10C, xylanase 11 A, xylanase 11A, xylanase 11J, xylanase 2, Xylanase 22, xylanase 43A, xylanase A, xylanase B, xylanase bI, xylanase bII, xylanase C, xylanase I, xylanase II, xylanase III, xylanase J, xylanase LC9, xylanase Xyl10A, xylanase Xyl11A, xylanase XynZF-2, xylanase, endo-1,4-, XylB, XylB8, XylC, XYLD, XylE, XylF2, XylG, xylH, xylM, XylX, XYLY, Xyn II, Xyn III, Xyn-b39, Xyn-Lxy, Xyn1, Xyn10, Xyn10A, Xyn10B, Xyn10E, XYN10G5, XYN10Ks_480, Xyn11, Xyn11A, XYN11F63, XYN11Ks_480, Xyn11NX, Xyn12.2, Xyn162, Xyn2, Xyn3, Xyn30A, Xyn5, XynA, XynA119, XynA19, XynA4, XynAS27, XynAS9, XynB, XynB18, XynBS27, XynC, XynD, XynE15, XynE2, XynG1, XynGH30, XynGR40, XYNII, XynIII, XynJ, XynS14, XynS20, XynSW1, XynSW3, XynT, XynY, XynZ, Xys1, Xys1delta, xysA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.8 endo-1,4-beta-xylanase

Cloned

Cloned on EC 3.2.1.8 - endo-1,4-beta-xylanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66
Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). Three Abf62A-Axe6A derivatives are constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: 1. RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide, 2. RjAbf62A-CBM6, containing the GH62 and CBM6 modules, and 3. RjAxe6A, containing only CE6
-
amplified fragment of Xyn11 (nucleotides 64-646) cloned into the pSE420-CHis vector and transformed into Escherichia coli strain XL1-Blue MR
cloning in Escherichia coli strain DH10B, overexpression of wild-type and mutant enzymes in Pichia pastoris strain X-33
-
constitutive expression in Pichia pastoris
-
construction of endoglucanase GE40 (EC 3.2.1.4) from Geobacillus thermodenitrificans with a signal peptide sequence derived from the endoxylase encoding gene GtxynA1 from Geobacillus thermodenitrificans strain T12, overview. Recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) (under control of the lac operator) and in Geobacillus thermodenitrificans strain T12
CtXynGH30, recombinant overexpression in Escherichia coli strain BL21
-
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3)
DNA fragments of xynS20 genes subcloned into vector pOSP2. XynS20 expressed as a recombinant protein fused to the N-terminus of oleosin by a linker polypeptide, intein M, in Escherichia coli
Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37°C, Luria-Bertoni broth
expressed from vector pET29-MS14-X11 in Escherichia coli strain BL21(DE3)pLysE
expressed from vector pT-xyl in Escherichia coli strain BL21(DE3)CodonPlus-RIL
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 (pLys) cells as glutathione S-transferase fusion protein
expressed in Escherichia coli BL21 Codon Plus (DE3) RIPL cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21/pET21a
expressed in Escherichia coli strain BL21 Codon plus
expressed in Escherichia coli SURE cells
-
expressed in Escherichia coli WK6 cells
-
expressed in Pichia pastoris
expressed in Pichia pastoris strain GS115
-
expressed in Pichia pastoris strain KM71
-
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21 with a 3C protease-removable N-terminal hexahistidine tag
expression in Escherichia coli BL21(DE3)SlyD
expression in Penicillium canescens
expression in Pichia pastoris
expression in Pichia pastoris, after codon optimization
expression in Pichia pastoris, yields a high titer of extracellular xylanase (502 U/ml) on induction with methanol
Halalkalibacterium halodurans
expression in Saccharomyces cerevisiae
-
expression in Streptomyces lividans TK24
expression in Streptomyces lividans, Streptomyces albus and Streptomyces parvulus
-
expression of C-terminally His-tagged wild-type, full-length Xyn10A, and of the isolated catalytic domain. Binding of the C-terminal 6His tag to the surface of XYN10A xylanase probably prevents unfolding from the C-terminus
-
expression of gene xyn2 and hybrid gene in Pichia pastoris under control of the inducible alcohol oxidase 1, AOX1, promoter using the secretion signal sequence from Saccharomyces cerevisiae, subcloning in Escherichis coli strain DH5alpha
-
expression of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus in Yarrowia lipolytica
expression of the hybrid gene in Pichia pastoris under control of the inducible alcohol oxidase 1, AOX1, promoter using the secretion signal sequence from Saccharomyces cerevisiae, subcloning in Escherichia coli strain DH5alpha
-
expression of wild-type and mutant AnxBs in Escherichia coli strain BL21 (DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(lambdaDE3)
expression of wild-type and mutant enzymes in Escherichia coli XL1-Blue
-
expression of wild-type and mutant SlxBs in Escherichia coli strain BL21 (DE3)
-
expression of wild-type and mutant SoxBs in Escherichia coli strain BL21 (DE3)
-
expresssed in Escherichia coli WK6 cells
full-length EGXA cloned into pFastBac vector and transformed into bacmid-containing Escherichia coli DH10Bac cells. Heterogeneously expressed in insect Tn5 cells
gene ctendo7, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115
Thermochaetoides thermophila
gene KRICT PX1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, expression in Escherichia coli strain BL21, subcloning in strain JM109
gene Kxyn, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3)
gene Mxyn10, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Esccherichia coli strain BL21(DE3)
gene MYCTH_49824, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter
gene MYCTH_56237, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter
gene Pedsa_2704, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TOP10
gene PgXynB, subcloning in Escherichia coli strain DH5alpha, expression of GST- or His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene TERTU_4506, recombinant expression of codon-optimized N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xyl1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree
gene xyl1, expression in Pichia pastoris
gene xyl10C from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris
gene Xyl10_1479, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Streptomyces lividans strain 1326, the enzyme is secreted
gene Xyl11_1020, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Streptomyces lividans strain 1326, the enzyme is secreted
gene xylA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
gene xylD, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH10B, expression in Pichia pastoris GS115
gene xylG, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21
gene xylX, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli
gene xyn10B, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
gene xyn10B, sequence comparisons, recombinant expression in Escherichia coli strain BL21 CodonPlus (RIPL)
gene xyn10G5, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
gene xyn11A, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, expression of the gene with intron, and the gene without intron under the control of AOX1 promoter in Pichia pastoris strain GS115, the enzyme is secreted to the culture medium
Thermochaetoides thermophila
gene xyn11F63, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Pichia pastoris, the recombinant enzyme is secreted to the culture medium
gene xyn11NX, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xyn2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme without N-terminal signal in Escherichia coli
B2CNY5
gene xyn2, expression in Pichia pastoris strain PX-1
-
gene Xyn2, expression in Pichia pastoris under the control of the methanol inducible alcohol oxidase 1, AOX1, promoter. The recombiannt enzyme is secreted to the culture medium
B2CNY5
gene xyn2, expressionin Pichia pastoris
-
gene xynA, DNA and amino acid sequence determination and analysis, expression of domains D1 + D2 or D2 alone in Escherichia coli
gene xynA, DNA and amino acid sequence determination and analysis, recombinant expression in Yarrowia lipolytica under control of the strong constitutive TEF promoter with the preproLIP2 secretion signal using plasmid JMP62_TEF_Ura3Ex, the enzyme is secreted. Recombinant expression in Pichia pastoris strain GS115 results in only low enzyme activity
gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons
-
gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His- and c-myc-tagged enzyme in Pichia pastoris strain GS115, the enzyme is secreted
gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in of His-tagged XynA in Escherichia coli strain DH10B and JM109
gene xynA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xynA, expression in Escherichia coli BL21(DE3)
-
gene xynA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene XynA, overexpression in Pichia pastoris, method optimization, functional expression under control of the AOX1 promotor
gene xynA119, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3), the recombinant xylanase is secreted to the culture supernatant
gene xynA19, phylogenetic tree, expression of His-tagged XynA19 without the signal peptide sequence in Escherichia coli strains BL21 (DE3) and Top10
gene xynA4, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xynB, constitutive expression of wild-type enzyme and enzyme without signal sequence in Pichia pastoris strain X-33, the recombinant enzyme is secreted to the culture medium, nmethod optimization, overview
gene xynB, DNA and amino acid sequence determination and analysis,over expression in Escherichia coli strain BLR(DE3)
gene xynB, expression as His-tagged enzyme in Escherichia coli BL21
-
gene xynB, expression in Saccharomyces cerevisiae strain SEY2102, the recombinant enzyme is secreted, and the extracellular endoxylanase expressed in yeast shows an enhanced thermal stability due to the N-linked glycosylation
gene xynC, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli
gene xynD, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X33
gene xynD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted
gene xynS14, DNA and amino acid sequence determination and analysis, expression of His-tagged XynS14 in Escherichia coli and Pichia pastoris
-
gene xynZ, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli
gene xysA, recombinant expression of C-terminally His-tagged Xys1 in Streptomyces lividans, the enzyme is secreted
heterologously expressed in Pichia pastoris
intact, CBM-truncated and CBM-linker-truncated versions cloned into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3)
into pET-22b(+) vector and expressed in Escherichia coli BL21 (DE3)
into pGEM T-easy and pET-21a vector, expressed in Escherichia coli XL10-Gold and BL21 (DE3)
into the pPIC9 vector and overexpressed in Pichia pastoris GS115
-
into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3)
into vector pUC119 and transformation of Escherichia coli DH5alpha. Into the NcoI site of pTrc99A and expressed in Escherichia coli BL21 CodonPlusR-RIL
-
library construction, cDNA isolation, DNA and amino acid sequence determination
mutants overexpressed in Escherichia coli
-
overexpressed in Streptomyces lividans
overexpression in Pichia pastoris
overexpression in Streptomyces lividans
-
partial DNA and amino acid sequencing
-
plasmids pET-DELTAXBD, pET-DELTAXBDR5 and pET-DELTAXBDK51R/R5 expressed in Escherichia coli BL21(DE3)
pUC19 plasmid, carrying the 10-kb HindIII fragment containing the xylanase gene expressed in Escherichia coli DH5alpha
recombinant expression in Escherichia coli
-
RuCelA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
specific and exact size fragment cloned into vector pTZ57R for transformation into Escherichia coli DH5alpha. Cloned xyn11A gene excised from pTZ57R with HindIII and EcoRI and ligated into pET-32a (+) and expressed in Escherichia coli BL21 under T7 promoter
Thermochaetoides thermophila
TfxA-cat gene cleaved by BamHI and PstI and ligated with vector pQE30
-
the chimeric enzymes, CorA-XynA and CorA-XynAG3, the parental enzyme XynA, and the thermostable variant XynAG3 are produced in Escherichia coli
-
the endo-beta-1,4-xylanase 2 gene is cloned from strain F3, then incorporated into the Fusarium oxysporum F3 genome again under the regulation of the gpdA promoter of Aspergillus nidulans. The transformation is effected through Agrobacterium tumefaciens. The transformants all show extracellular enzyme activity. Both transformants produce approximately 60% more ethanol compared to the wild type on corn cob, while for wheat bran this picture is repeated for only one of them, high extracellular xylanase activities in the transformants’ fermentation broths are maintained 2–2.5fold higher compared to the wild-type
-
the mutant gene xynZFTA is cloned into pPIC9K and expressed in Pichia pastoris
two catalytic domains XYLA-A and XYL-C, expressed separately as truncated gene products from lacZ fusion in Escherichia coli
-
wild-type and mutants introduced into the pGEX-1kT/PgXynA plasmid and expressed in Escherichia coli BL21 (pLys) strain
-
xylanase (XynB) and alpha-glucuronidase (AguA) from Thermotoga maritima are coproduced in Escherichia coli through dual-promoter and bicistronic constructs
xynA cloned from a genomic library into pBluescript. Extraneous fungal DNA upstream and downstream of xynA and the lacZ fusion partner removed by PCR. After PCR, cleavage and recloning, and the plasmid transformed into Escherichia coli XL1 Blue MRF'
-
xynB cDNA functionally expressed in Pichia pastoris GS115
XynBE18 expression in Escherichia coli strain Bl21(DE3)
-