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3.2.1.32: endo-1,3-beta-xylanase

This is an abbreviated version!
For detailed information about endo-1,3-beta-xylanase, go to the full flat file.

Word Map on EC 3.2.1.32

Reaction

(Xylbeta(1-3))x
+
H2O
=
(Xylbeta(1-3))x-y
 +
(Xylbeta(1-3))y

Synonyms

1,3-beta-D-xylan xylanohydrolase, 1,3-beta-xylanase, 1,3-xylanase, beta-1,3-xylanase, endo-1,3-beta-xylanase, endo-1,3-xylanase, endo-beta-1,3-xylanase, More, TxyA, XYL4, Xyl512, xylanase, xylanase, endo-1,3-, xylII, Xyn26A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.32 endo-1,3-beta-xylanase

Purification

Purification on EC 3.2.1.32 - endo-1,3-beta-xylanase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
6 different enzyme forms: EF-1, EF-2, EF-3, EF-4, EF-5 and EF-6
-
HisTrap HP column chromatography and HiTrap Q column chromatography
nickel affinity column chromatography and Superdex 200 gel filtration
recombinant
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL2(DE3) by nickel affinity and beta-1,3-xylan affinity chromatography and gel filtration