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Literature summary for 3.2.1.32 extracted from

  • Araki, T.; Inoue, N.; Morishita, T.
    Purification and characterization of beta-1,3-xylanase from a marine bacterium Alcaligenes sp. XY-234 (1998), J. Gen. Appl. Microbiol., 44, 269-274.
    View publication on PubMed
Search for more literature for 3.2.1.32

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, over 90% inhibition Alcaligenes sp.
Al3+ 1 mM AlCl3, strong Alcaligenes sp.
Cu2+ 1 mM CuCl2, strong Alcaligenes sp.
Fe3+ 1 mM FeCl3, strong Alcaligenes sp.
Hg2+ 1 mM, strong Alcaligenes sp.
N-bromosuccinimide
-
 Alcaligenes sp.
Pb(CH3COO)2 1 mM, strong Alcaligenes sp.
Pb2+ 1 mM, over 90% inhibition Alcaligenes sp.
Zn2+ 1 mM, over 90% inhibition Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, SDS-PAGE Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
 XY-234
-
Alcaligenes sp. XY-234
-
 XY-234
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.5
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O
-
 Alcaligenes sp. xylose + xylooligosaccharides
-
 ?
beta-1,3-xylan + H2O
-
 Alcaligenes sp. XY-234 xylose + xylooligosaccharides
-
 ?
additional information no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside Alcaligenes sp. ?
-
 ?
additional information no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside Alcaligenes sp. XY-234 ?
-
 ?
xylopentaose + H2O
-
 Alcaligenes sp. xylose + xylotetraose + xylobiose + xylotriose
-
 ?
xylopentaose + H2O
-
 Alcaligenes sp. XY-234 xylose + xylotetraose + xylobiose + xylotriose
-
 ?
xylotetraose + H2O
-
 Alcaligenes sp. xylose + xylotriose + xylobiose
-
 ?
xylotetraose + H2O
-
 Alcaligenes sp. XY-234 xylose + xylotriose + xylobiose
-
 ?
xylotriose + H2O
-
 Alcaligenes sp. xylose + xylobiose
-
 ?
xylotriose + H2O
-
 Alcaligenes sp. XY-234 xylose + xylobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.5, 10 min, stable below Alcaligenes sp.
50
-
 pH 7.5, 10 min, 70% loss of activity Alcaligenes sp.
60
-
 pH 7.5, 10 min, 99% loss of activity Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Alcaligenes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 4°C, 24 h, 35% loss of activity Alcaligenes sp.
6 10 4°C, 24 h, enzyme retains 90-100% of activity Alcaligenes sp.
12
-
 4°C, 24 h, retains 70% of its activity Alcaligenes sp.