Any feedback?
Literature summary for 3.2.1.32 extracted from
- Biochemical characterization of a thermostable beta-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359 (2013), Appl. Microbiol. Biotechnol., 97, 6749-6757.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermotoga neapolitana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E126Q | inactive | Thermotoga neapolitana |
| E126Q/E238Q | inactive | Thermotoga neapolitana |
| E238Q | inactive | Thermotoga neapolitana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Thermotoga neapolitana |  |
| Cu2+ | complete inhibition at 1 mM | Thermotoga neapolitana | |
| Fe2+ | complete inhibition at 1 mM | Thermotoga neapolitana | |
| Mn2+ | complete inhibition at 1 mM | Thermotoga neapolitana | |
| additional information | dithiothreitol, and EDTA have no significant effects on enzyme activity | Thermotoga neapolitana | |
| N-bromosuccinimide | complete inhibition at 1 mM | Thermotoga neapolitana | |
| Ni2+ | 45-55% inhibition at 1 mM | Thermotoga neapolitana | |
| Zn2+ | complete inhibition at 1 mM | Thermotoga neapolitana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+, Mg2+, and Na+ have no significant effects on enzyme activity | Thermotoga neapolitana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Thermotoga neapolitana |
| 38033 | - |
x * 38033, calculated from amino acid sequence | Thermotoga neapolitana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-xylan + H2O | Thermotoga neapolitana | - |
beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
| beta-1,3-xylan + H2O | Thermotoga neapolitana DSM 4359 | - |
beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | B9K760 | - |
- |
| Thermotoga neapolitana DSM 4359 | B9K760 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap HP column chromatography and HiTrap Q column chromatography | Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-xylan + H2O | - |
Thermotoga neapolitana | beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
| beta-1,3-xylan + H2O | best substrate | Thermotoga neapolitana | beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
| beta-1,3-xylan + H2O | - |
Thermotoga neapolitana DSM 4359 | beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
| beta-1,3-xylan + H2O | best substrate | Thermotoga neapolitana DSM 4359 | beta-1,3-xylobiose + beta-1,3-xylotriose + ? | - |
? | |
| glycol beta-1,3-xylan + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| glycol beta-1,3-xylan + H2O | - |
Thermotoga neapolitana DSM 4359 | ? | - |
? | |
| additional information | the enzyme does not hydrolyze beta-1,4-xylan, Avicel, carboxymethyl cellulose, laminarin, beta-1,4-mannan, starch, or various 4-nitrophenyl (pNP)-glycosides (pNP-alpha-glucosides, pNP-alpha-glucosides, pNP-alpha-galactosides, pNP-beta-galactosides, pNP-alpha-mannosides, pNP-beta-mannosides, pNP-alpha-xyloside, pNP-beta-xylosides, pNP-alpha-fucosides and pNP-beta-fucosides) | Thermotoga neapolitana | ? | - |
? | |
| additional information | the enzyme does not hydrolyze beta-1,4-xylan, Avicel, carboxymethyl cellulose, laminarin, beta-1,4-mannan, starch, or various 4-nitrophenyl (pNP)-glycosides (pNP-alpha-glucosides, pNP-alpha-glucosides, pNP-alpha-galactosides, pNP-beta-galactosides, pNP-alpha-mannosides, pNP-beta-mannosides, pNP-alpha-xyloside, pNP-beta-xylosides, pNP-alpha-fucosides and pNP-beta-fucosides) | Thermotoga neapolitana DSM 4359 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Thermotoga neapolitana |
| ? | x * 38033, calculated from amino acid sequence | Thermotoga neapolitana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-beta-D-xylan xylanohydrolase | - |
Thermotoga neapolitana |
| beta-1,3-xylanase | - |
Thermotoga neapolitana |
| Xyn26A | - |
Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 85 | the enzyme activity half-life is 23.9 h at 85°C and retains over 80% of initial activity at 80°C for 24 h | Thermotoga neapolitana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 322.6 | - |
glycol beta-1,3-xylan | at pH 6.5 and 85°C | Thermotoga neapolitana | |
| 588.2 | - |
beta-1,3-xylan | at pH 6.5 and 85°C | Thermotoga neapolitana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermotoga neapolitana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the enzyme remains stable at pH 3.0-10.0 after incubation at 4°C for 20 h | Thermotoga neapolitana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermotoga neapolitana | calculated from amino acid sequence | - |
5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
