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Literature summary for 3.2.1.32 extracted from

  • Kiyohara, M.; Sakaguchi, K.; Yamaguchi, K.; Araki, T.; Nakamura, T.; Ito, M.
    Molecular cloning and characterization of a novel beta-1,3-xylanase possessing two putative carbohydrate-binding modules from a marine bacterium Vibrio sp. strain AX-4 (2005), Biochem. J., 388, 949-957.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.32

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes are expressed in Escherichia coli Vibrio sp.

Protein Variants

Protein Variants Comment Organism
E138D activity is extremely low Vibrio sp.
E138Q inactive mutant Vibrio sp.
E234D activity is extremely low Vibrio sp.
E234Q inactive mutant Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM Vibrio sp.
Hg2+
-
 Vibrio sp.
Mn2+ 1 mM Vibrio sp.
Pb2+ 1 mM Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
 xylotetraose pH 7.5, 37°C Vibrio sp.
0.75
-
 xylopentaose pH 7.5, 37°C Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
-
 Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O
-
 Vibrio sp. disaccharides + trisaccharides + tetrasaccharides disaccharides, trisaccharides and tetrasaccharides are the major products, whereas the monosaccharides, pentasaccharides and oligosaccharides with more than five xylose units are produces at small quantities ?
glycol-beta-1,3-xylan + H2O
-
 Vibrio sp. xylose + ?
-
 ?
xylopentaose + H2O
-
 Vibrio sp. xylose + ?
-
 ?
xylotetraose + H2O
-
 Vibrio sp. xylose + ?
-
 ?

Synonyms

Synonyms Comment Organism
XYL4
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Vibrio sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 60 37°C: optimum, 60°C: does not hydrolyze beta-1,3-xylan above 60°C at pH 7.5 Vibrio sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
350
-
 xylotetraose pH 7.5, 37°C Vibrio sp.
14000
-
 xylopentaose pH 7.5, 37°C Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Vibrio sp.