3.1.21.2: deoxyribonuclease IV
This is an abbreviated version!
For detailed information about deoxyribonuclease IV, go to the full flat file.
Word Map on EC 3.1.21.2
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3.1.21.2
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exonuclease
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ape1
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glycosylase
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abasic
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strand
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endonucleases
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single-stranded
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nick
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uracil
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duplex
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phosphodiester
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uracil-dna
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methanesulfonate
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3'-blocking
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8-oxoguanine
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3'-phosphatase
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depurinated
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deoxyribose
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3\'-hydroxyl
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xrcc1
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diesterase
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incise
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site-containing
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base-excision
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endonucleolytic
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8-oxog
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soxrs
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ap-site
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5'-deoxyribose
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cross-complementing
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non-ltr
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repair-deficient
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exoiiis
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5'-phosphorylated
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uracil-containing
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non-long
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3'-phosphoglycolate
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asp148glu
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analysis
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pharmacology
- 3.1.21.2
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exonuclease
- ape1
- glycosylase
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abasic
- strand
- endonucleases
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single-stranded
- nick
- uracil
- duplex
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phosphodiester
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uracil-dna
- methanesulfonate
-
3'-blocking
- 8-oxoguanine
-
3'-phosphatase
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depurinated
- deoxyribose
-
3\'-hydroxyl
- xrcc1
- diesterase
-
incise
-
site-containing
-
base-excision
-
endonucleolytic
-
8-oxog
-
soxrs
-
ap-site
-
5'-deoxyribose
-
cross-complementing
-
non-ltr
-
repair-deficient
- exoiiis
-
5'-phosphorylated
-
uracil-containing
-
non-long
-
3'-phosphoglycolate
-
asp148glu
- analysis
- pharmacology
Reaction
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products =
Synonyms
apurinic/apyrimidinic endonuclease, coliphage T4 endonuclease II, deoxriboendonuclease, DNA-adenine-transferase, E. coli endonuclease IV, EC 3.1.4.30, End, Endo IV, endodeoxyribonuclease IV, EndoII, EndoIV, endonuclease II, endonuclease IV, exonuclease III, More, MtbEndo IV, MtbNfo, MtbXthA, Nfo, nuclease, endodeoxyribo-oder redoxyendonuclease, Rv0670, Sco4631, ScoA3McrA, T4 endonuclease IV, TthNfo, type IV methyl-dependent restriction endonuclease
ECTree
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Engineering
Engineering on EC 3.1.21.2 - deoxyribonuclease IV
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G149D
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site-directed mutagenesis, the mutant is deficient in both nucleotide incision repair and exonuclease activities
H69A
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site-directed mutagenesis, the mutant is deficient in both nucleotide incision repair and exonuclease activities. The crystal structure of Nfo-H69A mutant reveals the loss of one of the active site zinc atoms and rearrangements of the catalytic site, but no gross changes in the overall enzyme conformation
G149D
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site-directed mutagenesis, the mutant is deficient in both nucleotide incision repair and exonuclease activities
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H69A
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site-directed mutagenesis, the mutant is deficient in both nucleotide incision repair and exonuclease activities. The crystal structure of Nfo-H69A mutant reveals the loss of one of the active site zinc atoms and rearrangements of the catalytic site, but no gross changes in the overall enzyme conformation
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H508A
S176N
Tequatrovirus T4
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mutant enzyme retains cleavage activity (17.5% of that of wild-type Endo IV), but loses the polarized and restricted cleavage of a dCs tract. Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, the mutant enzyme does not show detrimental effect on the host cells
W88R
Tequatrovirus T4
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mutant enzyme shows no enzymatic activity (less than 0.4% of that of wild-type Endo IV). Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, these mutant enzymes do not show detrimental effect on the host cells
additional information
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analysis of enzyme activity in naturally occurring mutants of Escherichia coli compared to wild-type strain enzymes, overview