3.1.21.2: deoxyribonuclease IV
This is an abbreviated version!
For detailed information about deoxyribonuclease IV, go to the full flat file.
Word Map on EC 3.1.21.2
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3.1.21.2
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exonuclease
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ape1
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glycosylase
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abasic
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strand
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endonucleases
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single-stranded
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nick
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uracil
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duplex
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phosphodiester
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uracil-dna
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methanesulfonate
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3'-blocking
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8-oxoguanine
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3'-phosphatase
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depurinated
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deoxyribose
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3\'-hydroxyl
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xrcc1
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diesterase
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incise
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site-containing
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base-excision
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endonucleolytic
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8-oxog
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soxrs
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ap-site
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5'-deoxyribose
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cross-complementing
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non-ltr
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repair-deficient
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exoiiis
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5'-phosphorylated
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uracil-containing
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non-long
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3'-phosphoglycolate
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asp148glu
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analysis
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pharmacology
- 3.1.21.2
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exonuclease
- ape1
- glycosylase
-
abasic
- strand
- endonucleases
-
single-stranded
- nick
- uracil
- duplex
-
phosphodiester
-
uracil-dna
- methanesulfonate
-
3'-blocking
- 8-oxoguanine
-
3'-phosphatase
-
depurinated
- deoxyribose
-
3\'-hydroxyl
- xrcc1
- diesterase
-
incise
-
site-containing
-
base-excision
-
endonucleolytic
-
8-oxog
-
soxrs
-
ap-site
-
5'-deoxyribose
-
cross-complementing
-
non-ltr
-
repair-deficient
- exoiiis
-
5'-phosphorylated
-
uracil-containing
-
non-long
-
3'-phosphoglycolate
-
asp148glu
- analysis
- pharmacology
Reaction
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products =
Synonyms
apurinic/apyrimidinic endonuclease, coliphage T4 endonuclease II, deoxriboendonuclease, DNA-adenine-transferase, E. coli endonuclease IV, EC 3.1.4.30, End, Endo IV, endodeoxyribonuclease IV, EndoII, EndoIV, endonuclease II, endonuclease IV, exonuclease III, More, MtbEndo IV, MtbNfo, MtbXthA, Nfo, nuclease, endodeoxyribo-oder redoxyendonuclease, Rv0670, Sco4631, ScoA3McrA, T4 endonuclease IV, TthNfo, type IV methyl-dependent restriction endonuclease
ECTree
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Crystallization
Crystallization on EC 3.1.21.2 - deoxyribonuclease IV
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wild-type enzyme and mutant H69A, hanging-drop vapor diffusion method, mxing of 0.0015 ml of protein solution containing 10 mg/ml protein with 0.002 ml of reservoir solution containing 35% PEG 400 and 100 mM HEPES, pH 5.5, 20°C, 7 days, X-ray diffraction structure determination and analysis at 1.55 A resolution
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sitting-drop vapor-diffusion method at 18°C, the structure of the recombinant endonuclease IV is determined at 1.18 A resolution
mutant E118A of EndoII is crystallized in space group P21 with four monomers in the asymmetric unit, with a bound phosphate ion in one of the four active sites of EndoII likely mimicing the scissile phosphate in a true substrate complex. Crystallization of the selenomethionine-substituted E118A mutant, X-ray diffraction structure determination and analysis at 1.9-2.3 A resolution, molecular replacement
Tequatrovirus T4
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hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 9.0, 4 mM dithiothreitol, 1.5% saturated MgSO4, 16% (w/v) ethylene glycol, 20% (w/v) polyethylene glycol monomethyl ether 2000