Cloned (Comment) | Organism |
---|---|
gene sco4631, expression in Streptomyces lividans results in the loss of the genomic island that contains the dndA-E gene cluster, expression of N-terminally His6-tagged Sco4631 in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
H508A | catalytically inactive mutant | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for activity | Streptomyces coelicolor | |
Mn2+ | required for activity | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces coelicolor | a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links | ? | - |
? | |
additional information | Streptomyces coelicolor A(3)2 | a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
gene sco4631 | - |
Streptomyces coelicolor A(3)2 | - |
gene sco4631 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links | Streptomyces coelicolor | ? | - |
? | |
additional information | a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links | Streptomyces coelicolor A(3)2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sco4631 | - |
Streptomyces coelicolor |
ScoA3McrA | - |
Streptomyces coelicolor |
type IV methyl-dependent restriction endonuclease | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | in vitro endonuclease activity of the Escherichia coli McrA homologue that specifically cleaves S-modified DNA | Streptomyces coelicolor |