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Literature summary for 3.1.21.2 extracted from

  • Liu, G.; Ou, H.; Wang, T.; Li, L.; Tan, H.; Zhou, X.; Rajakumar, K.; Deng, Z.; He, X.
    Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA (2010), PLoS Genet., 6, 1-13.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene sco4631, expression in Streptomyces lividans results in the loss of the genomic island that contains the dndA-E gene cluster, expression of N-terminally His6-tagged Sco4631 in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
H508A catalytically inactive mutant Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required for activity Streptomyces coelicolor
Mn2+ required for activity Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces coelicolor a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links ?
-
?
additional information Streptomyces coelicolor A(3)2 a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
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gene sco4631
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Streptomyces coelicolor A(3)2
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gene sco4631
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links Streptomyces coelicolor ?
-
?
additional information a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links Streptomyces coelicolor A(3)2 ?
-
?

Synonyms

Synonyms Comment Organism
Sco4631
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Streptomyces coelicolor
ScoA3McrA
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Streptomyces coelicolor
type IV methyl-dependent restriction endonuclease
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Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function in vitro endonuclease activity of the Escherichia coli McrA homologue that specifically cleaves S-modified DNA Streptomyces coelicolor