3.1.13.3: oligonucleotidase
This is an abbreviated version!
For detailed information about oligonucleotidase, go to the full flat file.
Word Map on EC 3.1.13.3
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3.1.13.3
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exoribonuclease
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exonuclease
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trna
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oligoribonucleotides
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pnpase
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cyclic-di-gmp
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exonucleolytic
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phosphoesterase
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c-di-gmp
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deddh
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3'-phosphoadenosine
- 3.1.13.3
- exoribonuclease
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exonuclease
- trna
- oligoribonucleotides
- pnpase
- cyclic-di-gmp
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exonucleolytic
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phosphoesterase
- c-di-gmp
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deddh
- 3'-phosphoadenosine
Reaction
exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates =
Synonyms
CpsORN, EC 3.1.4.19, More, nanoRNase, NrnC, nucleotidase, oligo-, oligoribonuclease, ORN, phosphodiesterase, REXO2, RNase T, small fragment nuclease, XC847, YtqI
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Cloned
Cloned on EC 3.1.13.3 - oligonucleotidase
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expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI
expression in Escherichia coli
expression of his-tagged YtqI under control of the arabinose-inducible promoter Para
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gene orn, detailed phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene orn, quantitative RT-PCR expression analysis
gene orn, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94)
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expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI
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expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI
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expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI
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