3.1.13.3: oligonucleotidase
This is an abbreviated version!
For detailed information about oligonucleotidase, go to the full flat file.
Word Map on EC 3.1.13.3
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3.1.13.3
-
exoribonuclease
-
exonuclease
-
trna
-
oligoribonucleotides
-
pnpase
-
cyclic-di-gmp
-
exonucleolytic
-
phosphoesterase
-
c-di-gmp
-
deddh
-
3'-phosphoadenosine
- 3.1.13.3
- exoribonuclease
-
exonuclease
- trna
- oligoribonucleotides
- pnpase
- cyclic-di-gmp
-
exonucleolytic
-
phosphoesterase
- c-di-gmp
-
deddh
- 3'-phosphoadenosine
Reaction
exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates =
Synonyms
CpsORN, EC 3.1.4.19, More, nanoRNase, NrnC, nucleotidase, oligo-, oligoribonuclease, ORN, phosphodiesterase, REXO2, RNase T, small fragment nuclease, XC847, YtqI
ECTree
3.1.13.3 oligonucleotidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.13.3 - oligonucleotidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5'Cy5-CCCCC3' + H2O
?
5'Cy5-AAAAA3' is a better substrate than 5'Cy5-CCCCC3'
-
-
?
mRNA + H2O
nucleoside 5'-phosphates
-
required for for degradation of mRNA to mononucleotides
-
-
?
p-nitrophenyl deoxythymidine 5'-phosphate + H2O
p-nitrophenol + 5'-TMP
-
no activity on nitrophenyl deoxythymidine 3'-phosphate
-
-
?
p-nitrophenyl uridine 5'-monophosphate + H2O
p-nitrophenol + 5'-UMP
-
-
-
-
?
p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O
p-nitrophenol + isopropyl-5'-UMP
-
-
-
-
?
RNA + H2O
nucleoside 5'-phosphate + ?
-
degrades RNA oligonucleotides with preference for 3-mers
-
-
?
1,N6-etheno-2'-dApG + H2O
1,N6-etheno-2'-deoxyadenosine + GMP
-
-
-
?
1,N6-etheno-2'-dApG + H2O
1,N6-etheno-2'-deoxyadenosine + GMP
-
-
-
?
1,N6-etheno-2'-dApG + H2O
1,N6-etheno-2'-deoxyadenosine + GMP
-
-
-
?
4-nitrophenyl-TMP + H2O
4-nitrophenol + TMP
artificial assay substrate
-
-
?
4-nitrophenyl-TMP + H2O
4-nitrophenol + TMP
Colwellia psychrerythraea ATCC BAA-681
artificial assay substrate
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
-
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
5'Cy5-AAA3' + H2O
?
better substrate than 5'Cy5-CCCCC3'
-
-
?
5'Cy5-AAAAA3' + H2O
?
better substrate than 5'Cy5-CCCCC3'
-
-
?
5'Cy5-CCC3' + H2O
?
5'Cy5-AAA3' is a better substrate than 5'Cy5-CCC3'
-
-
?
5'Cy5-CCC3' + H2O
?
5'Cy5-AAA3' is a better substrate than 5'Cy5-CCC3'
-
-
?
DNA + H2O
nucleoside 5'-phosphates
-
active after dissociation from a ribonucleoprotein
-
-
?
DNA + H2O
nucleoside 5'-phosphates
-
hydrolyzed very slowly, not: ADP, UDP, ATP, UTP
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
-
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
processive degradation
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
however higher affinity to longer chains
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
exonuclease: 3`to 5'-direction
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
reaction rate inversely proportional to chain length of substrate
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
hydrolyzes oligodeoxyribonucleotides and oligoribonucleotides
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
specific for short ribooligonucleotides
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
initial site of attack: 3'-hydroxyl end
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
also hydrolyses NAD+ to NMN and AMP, mechanism
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
essential for complete degradation of mRNA
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
exonuclease: 3`to 5'-direction
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
hydrolyzes oligodeoxyribonucleotides and oligoribonucleotides
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
has higher affinity to shorter chains
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
no activity at 3'-phosphate end
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
hydrolyzes oligodeoxyribonucleotides and oligoribonucleotides
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
no base specificity
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
initial site of attack: 3'-hydroxyl end
-
-
?
RNA + H2O
nucleoside 5'-phosphates
-
active after dissociation from a ribonucleoprotein
-
-
?
additional information
?
-
oligo(A) RNA substrates are preferred over oligo(C) substrates, and RA 5mers are preferred over RNA 3mers
-
-
?
additional information
?
-
-
oligo(A) RNA substrates are preferred over oligo(C) substrates, and RA 5mers are preferred over RNA 3mers
-
-
?
additional information
?
-
oligo(A) RNA substrates are preferred over oligo(C) substrates, and RA 5mers are preferred over RNA 3mers
-
-
?
additional information
?
-
CpsORN activities on 5-mer RNA or DNA are determined using custom-made 5'-fluorescein-labelled oligonucleotides, and assay on 24-mer RNA oligonucleotide. Small RNA hydrolysis mechanism of CpsORN, mechanism, overview
-
-
?
additional information
?
-
Colwellia psychrerythraea ATCC BAA-681
CpsORN activities on 5-mer RNA or DNA are determined using custom-made 5'-fluorescein-labelled oligonucleotides, and assay on 24-mer RNA oligonucleotide. Small RNA hydrolysis mechanism of CpsORN, mechanism, overview
-
-
?
additional information
?
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
poor activity with 4-nitrophenyl phosphate
-
-
?
additional information
?
-
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
poor activity with 4-nitrophenyl phosphate
-
-
?
additional information
?
-
oligoribonuclease (Orn) is an exoribonuclease that hydrolyzes two- to five-nucleotide-long RNAs. Orn degrades short oligoRNAs regardless of sequence
-
-
?
additional information
?
-
-
oligoribonuclease (Orn) is an exoribonuclease that hydrolyzes two- to five-nucleotide-long RNAs. Orn degrades short oligoRNAs regardless of sequence
-
-
?
additional information
?
-
poor activity with 4-nitrophenyl phosphate
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
recombinant Orn degrades small RNAs in vitro, the enzyme has a proclivity for degrading RNA oligomers comprised of two to five nucleotides (nanoRNAs), including 5'-phosphoguanylyl-(3',5')-guanosine. Orn preferentially degrades purine dinucleotides in vitro. Exonuclease assays are carried out using 33P- and Cy5-labled RNA oligomer substrates. Orn is a calcium-insensitive exoribonuclease that selectively degrades purine rich nanoRNAs. (A) Degradation of the 24-mer 5'-33P-CACACACACA-CACACACACACACA-3'. Orn is a poor degrader of cytosine-rich nanoRNA substrates
-
-
?
additional information
?
-
-
degradation of RNA oligomers by oligoribonuclease is critical for completion of the Streptomyces coelicolor life cycle, but expression of ornA is not likely to be contigent on bldA-dependent translation of adpA
-
-
?
