3.1.13.3: oligonucleotidase

This is an abbreviated version!
For detailed information about oligonucleotidase, go to the full flat file.

Word Map on EC 3.1.13.3

3.1.13.3

exoribonuclease

exonuclease

trna

oligoribonucleotides

pnpase

cyclic-di-gmp

exonucleolytic

phosphoesterase

c-di-gmp

deddh

3'-phosphoadenosine

Reaction

exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates =

Synonyms

CpsORN, EC 3.1.4.19, More, nanoRNase, NrnC, nucleotidase, oligo-, oligoribonuclease, ORN, phosphodiesterase, REXO2, RNase T, small fragment nuclease, XC847, YtqI

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.13 Exoribonucleases producing 5′-phosphomonoesters

3.1.13.3 oligonucleotidase

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Results	in table
202	AA Sequence
3	Application
1	CAS Registry Number
14	Cloned(Commentary)
6	Crystallization (Commentary)
21	Disease/ Diagnostics
36	General Information
27	Inhibitors
3	Ki Value [mM]
14	KM Value [mM]
3	Localization
25	Metals/Ions
7	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
41	Organism
13	pH Optimum
3	pH Range
26	Protein Variants
15	Purification (Commentary)
3	Reaction
1	Reaction Type
41	Reference
1	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
119	Substrates and Products (Substrate)
15	Subunits
53	Synonyms
9	Temperature Optimum [°C]
4	Temperature Stability [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 3.1.13.3 - oligonucleotidase

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5 entries

copurifies with polynucleotide phosphorylase

Escherichia coli

134086

more than 99% pure

Xanthomonas campestris

682781

on Ni-NTA column and by gel filtration, more than 95% pure

3 entries

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Pseudomonas aeruginosa

P57665

752033

recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Colwellia psychrerythraea

to homogeneity