1.5.3.6: (R)-6-hydroxynicotine oxidase
This is an abbreviated version!
For detailed information about (R)-6-hydroxynicotine oxidase, go to the full flat file.
Word Map on EC 1.5.3.6
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1.5.3.6
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fad
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arthrobacter
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oxidans
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flavinylation
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holoenzyme
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flavin
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flavoproteins
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6-hydroxy-l-nicotine
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flavoenzymes
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apoenzyme
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nicotinovorans
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nicotine-degrading
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l-nicotine
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dimethylglycine
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glycerol-3-p
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cotranslational
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fad-binding
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l-enantiomer
- 1.5.3.6
- fad
- arthrobacter
- oxidans
-
flavinylation
-
holoenzyme
- flavin
- flavoproteins
- 6-hydroxy-l-nicotine
-
flavoenzymes
-
apoenzyme
- nicotinovorans
-
nicotine-degrading
- l-nicotine
- dimethylglycine
-
glycerol-3-p
-
cotranslational
-
fad-binding
-
l-enantiomer
Reaction
Synonyms
6-HDNO, 6-hydroxy-D-nicotine oxidase, D-6-hydroxynicotine oxidase
ECTree
Advanced search results
Engineering
Engineering on EC 1.5.3.6 - (R)-6-hydroxynicotine oxidase
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C136S
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5% of wild-type activity, slight activation with glycerol-3-phosphate and FAD
C136S/C260S
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no activity, no activation with glycerol-3-phosphate and FAD
C260S
-
15% of wild-type activity, no activation with glycerol-3-phosphate and FAD
C433S
C59S
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40% of wild-type activity, no activation with glycerol-3-phosphate and FAD
E350A
Q8GAG1
substitution decreases the kcat/Kamine value by about 20fold
E350Q
Q8GAG1
substitution decreases the kcat/Kamine value by about 220fold
E352Q
Q8GAG1
substitution decreases the kcat/Kamine value about 3800fold
H71C
K348M
Q8GAG1
substitution has only a small effect on the kinetic parameters
R67K
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3% activity of wild-type, better flavinylation rate than wild type enzyme
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40% of wild-type activity, 50% activation with glycerol-3-phosphate and FAD
C433S
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deletions F448 and R449, no activity, no activation with glycerol-3-phosphate and FAD