EC Number   |
Protein Variants |
Reference |
|---|
   1.5.3.6 | C136S |
5% of wild-type activity, slight activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | C136S/C260S |
no activity, no activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | C260S |
15% of wild-type activity, no activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | C433S |
40% of wild-type activity, 50% activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | C433S |
deletions F448 and R449, no activity, no activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | C59S |
40% of wild-type activity, no activation with glycerol-3-phosphate and FAD |
392449 |
| 1.5.3.6 | E350A |
substitution decreases the kcat/Kamine value by about 20fold |
764163 |
| 1.5.3.6 | E350Q |
substitution decreases the kcat/Kamine value by about 220fold |
764163 |
| 1.5.3.6 | E352Q |
substitution decreases the kcat/Kamine value about 3800fold |
764163 |
| 1.5.3.6 | H71C |
80% activity of wild-type |
392446 |
