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1.2.1.9: glyceraldehyde-3-phosphate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+), go to the full flat file.

Wordmap for 1.2.1.9

Word Map on EC 1.2.1.9 Wordmap for 1.2.1.9

Reaction

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D-glyceraldehyde 3-phosphate
+
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NADP+
+
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H2O
=
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3-phospho-D-glycerate
+
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NADPH
+
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H+

Synonyms

ALDH11A3, apo-GAPDH, apo-glyceraldehyde-3-phosphate dehydrogenase, At2g24270, CagapC, CbgapC, CsgapC, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate), E268A-GAPN, GAP dehydrogenase, GAPC, GAPDH, GAPDH (A4), GAPDHN, GAPN, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde 3-phosphate:NADP+ reductase, non-phosphorylating, glyceraldehyde phosphate dehydrogenase (NADP), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), Glyceraldehyde-3-phosphate dehydrogenase [NADP+], glyceraldehyde-3-phosphate:NADP reductase, NADP+-dependent GAPDH, NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP+-GAPDH, NADP-ALDH11A3, NADP-dependent ALDH11A3, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADPH-dependent GAPDH, NADPH-glyceraldehyde-3-phosphate dehydrogenase, non phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating GAP dehydrogenase, Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase GAPN, non-phosphorylating glyceraldehyde-phosphate dehydrogenase, non-phosphorylating NADP+-dependent Ga3PDHase, non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NP-Ga3PDHase, NP-GAPDH, OsALDH11, SSO3194, St-GAPN, STK_24770, triose phosphate dehydrogenase, Triosephosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                EC 1.2.1.91.2.1.9 glyceraldehyde-3-phosphate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.2.1.9 - glyceraldehyde-3-phosphate dehydrogenase (NADP+)

for references in articles please use BRENDA:EC1.2.1.9

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in BL21 (DE3) CodonPlus using the pET expression vector system
EcoRI-digested genomic DNA into plasmid pUC18 for sequencing of gapN, into an expression vector for recombinant production of the enzyme in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) CodonPlus cells
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells
-
expressed in Escherichia coli C43 (DE3) cells
expressed in Saccharomyces cerevisiae mutant AG2 with deletion of NAD+-dependent glycerol-3-phosphate dehydrogenase gene GPD2
-
expression by functional complementation of the Escherichia coli gap mutant W3CG
-
expression in Escherichia coli
expression in Escherichia coli BL-21
-
for expression in Escherichia coli
gene gapC, recombinant expression in Corynebacterium glutamicum strain KBJ11, method optimization, coexpression with gene BsrocG encoding NADH-dependent glutamate dehydrogenase from Bacillus subtilis strain HB-1, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
-
gene gapC, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN
into the pET29 vector for expression in Escherichia coli BL21DE3 cells
-
into the pGEM-T easy vector for sequencing
overexpression in Escherichia coli BL21(DE3)-pLysS
-