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Literature summary for 1.2.1.9 extracted from
- Presence of NADP+-specific glyceraldehyde-3-phosphate dehydrogenase and CcpA-dependent transcription of its gene in the ruminal bacterium Streptococcus bovis (2006), FEMS Microbiol. Lett., 257, 17-23.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | gapN transcription is enhanced by the catabolite control protein A, CcpA | Streptococcus equinus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| EcoRI-digested genomic DNA into plasmid pUC18 for sequencing of gapN, into an expression vector for recombinant production of the enzyme in Escherichia coli | Streptococcus equinus |
| for expression in Escherichia coli | Streptococcus equinus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51100 | - |
- |
Streptococcus equinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | Streptococcus equinus | - |
3-phospho-D-glycerate + NADPH + H+ | - |
? |  |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | Streptococcus equinus | - |
3-phospho-D-glycerate + NADPH + H+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus equinus | Q3C1A6 | - |
- |
| Streptococcus equinus | Q3C1A6 | Streptococcus bovis gapN, sequence of gapN gene of S. mutans and S. pyogenes used for PCR primer design, Q59931, Q99Z67 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
growth stage: growth cessation, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.008 | - |
growth stage: just before the cessation of growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.011 | - |
growth stage: later log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.014 | - |
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.014 | - |
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.015 | - |
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.015 | - |
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.016 | - |
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.016 | - |
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.028 | - |
strain 12IU1p, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.028 | - |
strain 12U1p, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.032 | - |
growth stage: middle log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.033 | - |
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.033 | - |
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.039 | - |
recombinant GAPN overexpression strain 12U1gapN-1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.039 | - |
strain 12U1gapN-1, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
| 0.065 | - |
recombinant GAPN overexpression strain 12U1gapN-2, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 | Streptococcus equinus |
| 0.065 | - |
strain 12U1gapN-2, microM NADP+ reduced min-1 (mg of cell-N)-1 | Streptococcus equinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Streptococcus equinus | 3-phospho-D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Streptococcus equinus | 3-phospho-D-glycerate + NADPH + H+ | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPN | - |
Streptococcus equinus |
| glyceraldehyde-3-phosphate dehydrogenase | - |
Streptococcus equinus |
| glyceraldehyde-3-phosphate dehydrogenase (NADP+) | - |
Streptococcus equinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
activity assay at room temperature | Streptococcus equinus |
| 23 | - |
activity assay at room temperature | Streptococcus equinus |
| 60 | - |
- |
Streptococcus equinus |
| 60 | - |
optimal temperature for Streptococcus bovis GAPN | Streptococcus equinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
activity assay | Streptococcus equinus |
| 8.5 | - |
optimal pH for Streptococcus bovis GAPN | Streptococcus equinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Streptococcus equinus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptococcus equinus | - |
- |
5.12 |
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