Any feedback?
Literature summary for 1.2.1.9 extracted from
- Purification and properties of NADP-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the green algae Chlamydomonas reinhardtii (1987), Biochim. Biophys. Acta, 925, 1-10.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphohydroxypyruvate | - |
Chlamydomonas reinhardtii |  |
| D-3-phosphoglycerate | competitive | Chlamydomonas reinhardtii | |
| D-erythrose 4-phosphate | - |
Chlamydomonas reinhardtii | |
| iodoacetamide | - |
Chlamydomonas reinhardtii | |
| L-Glyceraldehyde 3-phosphate | - |
Chlamydomonas reinhardtii | |
| NADPH | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0098 | - |
NADP+ | - |
Chlamydomonas reinhardtii | |
| 0.051 | - |
D-glyceraldehyde 3-phosphate | - |
Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | 200000 | gel filtration | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | Chlamydomonas reinhardtii | - |
D-3-phosphoglycerate + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 25 mM Tris-HCl buffer, pH 7.5, 0.1 mM EDTA, 10 mM mercaptoethanol, at least 1 month | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | - |
Chlamydomonas reinhardtii | D-3-phosphoglycerate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000-53000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
inactivation above | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Chlamydomonas reinhardtii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
