Cloned (Comment) | Organism |
---|---|
gene gapC, recombinant expression in Corynebacterium glutamicum strain KBJ11, method optimization, coexpression with gene BsrocG encoding NADH-dependent glutamate dehydrogenase from Bacillus subtilis strain HB-1, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Clostridium saccharobutylicum |
gene gapC, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Clostridium beijerinckii |
gene gapC, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Clostridium acetobutylicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CRISPR-Cpf1-assisted engineering of Corynebacterium glutamicum SNK118 for enhanced L-ornithine production by NADP-dependent glyceraldehyde-3-phosphate dehydrogenase and NADH-dependent glutamate dehydrogenase. Insufficient intracellular NADPH content has been recognized as a limiting factor in L-ornithine biosynthesis. Overexpression of NADP-dependent glyceraldehyde 3-phosphate dehydrogenase gene CsgapC from Clostridium saccharobutylicum strain DSM 13864 and NADH-dependent glutamate dehydrogenase gene BsrocG from Bacillus subtilis strain HB-1 results in markedly increased ornithine biosynthesis. The engineered Corynebacterium glutamicum strain KBJ11 (SNK118DELTAargRDELTAargFDELTAncgl2228/pXMJ19-CsgapC-BsrocG) is constructed for L-ornithine overproduction. In fed-batch fermentation, L-ornithine of 88.26 g/l with productivity of 1.23 g/l/h (over 72 h) and yield of 0.414 g/g glucose is achieved by strain KBJ11 in a 10-l bioreactor. High titer and yield of L-ornithine production by microbial fermentation proves that heterologous expression of CsgapC and BsrocG can promote L-ornithine production by Corynebacterium glutamicum strains | Clostridium saccharobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Clostridium acetobutylicum | - |
3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Clostridium saccharobutylicum | - |
3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Clostridium acetobutylicum 1.7 | - |
3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | Clostridium saccharobutylicum DSM 13864 | - |
3-phospho-D-glycerate + NADPH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | - |
- |
- |
Clostridium acetobutylicum 1.7 | - |
- |
- |
Clostridium beijerinckii | - |
- |
- |
Clostridium beijerinckii DSM 1739 | - |
- |
- |
Clostridium saccharobutylicum | - |
- |
- |
Clostridium saccharobutylicum DSM 13864 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified recombinant enzyme, pH 8.5, 30°C | Clostridium acetobutylicum |
5.263 | - |
purified recombinant enzyme, pH 8.5, 30°C | Clostridium saccharobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Clostridium acetobutylicum | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Clostridium saccharobutylicum | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Clostridium acetobutylicum 1.7 | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Clostridium saccharobutylicum DSM 13864 | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
additional information | recombinant CbgapC shows no catalytic activity | Clostridium beijerinckii | ? | - |
- |
|
additional information | recombinant CbgapC shows no catalytic activity | Clostridium beijerinckii DSM 1739 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CagapC | - |
Clostridium acetobutylicum |
CbgapC | - |
Clostridium beijerinckii |
CsgapC | - |
Clostridium saccharobutylicum |
GAPC | - |
Clostridium beijerinckii |
GAPC | - |
Clostridium acetobutylicum |
GAPC | - |
Clostridium saccharobutylicum |
NADP-dependent glyceraldehyde 3-phosphate dehydrogenase | - |
Clostridium beijerinckii |
NADP-dependent glyceraldehyde 3-phosphate dehydrogenase | - |
Clostridium acetobutylicum |
NADP-dependent glyceraldehyde 3-phosphate dehydrogenase | - |
Clostridium saccharobutylicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Clostridium beijerinckii |
30 | - |
assay at | Clostridium acetobutylicum |
30 | - |
assay at | Clostridium saccharobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Clostridium beijerinckii |
8.5 | - |
assay at | Clostridium acetobutylicum |
8.5 | - |
assay at | Clostridium saccharobutylicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Clostridium beijerinckii | |
NADP+ | - |
Clostridium acetobutylicum | |
NADP+ | - |
Clostridium saccharobutylicum |