1.2.1.13: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) This is an abbreviated version! For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating), go to the full flat file .
Reaction
D-glyceraldehyde 3-phosphate +
phosphate +
NADP+ =
3-phospho-D-glyceroyl phosphate +
NADPH +
H+
Synonyms A2B2-GAPDH, A2B2-glyceraldehyde-3-phosphate dehydrogenase, A4 glyceraldehyde 3-phosphate dehydrogenase, A4-GAPDH, At1g12900, At3g26650, D-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating), GapA, GapA-1, GAPA1, GAPA2, GapB, GAPC, GAPD, GAPDH, GAPDH (A4), glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating), glyceraldehyde-3-dehydrogenase, glyceraldehyde-3-P dehydrogenase, glyceraldehyde-3-phosphate dehhydrogenase (NADP+) (phoshphorylating), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), glyceraldehyde-P dehydrogenase, GraP-DH, NAD(P)-GAPDH, NADP(H)-glyceraldehyde-3-phosphate dehydrogenase, NADP+-dependent G-3-P dehydrogenase, NADP+-dependent gapB, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent GAPDH, NADP-dependent glyceradehdye-3-phosphate dehydrogenase, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP-dependent glyceraldehyde phosphate dehydrogenase, NADP-dependent glyceraldehydephosphate dehydrogenase, NADP-G3PDH, NADP-GAPDH, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADP-GPD, NADP-triose phosphate dehydrogenase, NADPH-D-GA3P, NADPH-glyceraldehyde-3-phosphate dehydrogenase, NAPD-linked glyceraldehyde-3-P dehydrogenase, Ov-GAPDH, phosphorylating GAP dehydrogenase, phosphorylating glyceraldehyde-3-phosphate dehydrogenase, photosynthetic GAPDH, SSO0528, triosephosphate dehydrogenase (NADP+)
ECTree
Turnover Number
Turnover Number on EC 1.2.1.13 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)
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40 - 419
3-phospho-D-glyceroyl phosphate
additional information
additional information
-
40
3-phospho-D-glyceroyl phosphate
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH
88
3-phospho-D-glyceroyl phosphate
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH
223
3-phospho-D-glyceroyl phosphate
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH
419
3-phospho-D-glyceroyl phosphate
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH
8.4
NADH
-
mutant enzyme R190A, pH and temperature not specified in the publication
32
NADH
mutant enzyme S188A
37
NADH
mutant enzyme T33A
40
NADH
-
mutant enzyme K128A
41
NADH
native enzyme A3-GAPDH
41
NADH
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase
48
NADH
mutant enzyme T33A/S188A
56
NADH
-
mutant enzyme K128E
71
NADH
-
mutant enzyme R82A, pH and temperature not specified in the publication
104
NADH
-
wild-type enzyme
104
NADH
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase
104
NADH
-
wild type enzyme, pH and temperature not specified in the publication
108
NADH
-
mutant enzyme R197A
119
NADH
-
mutant enzyme S195A, pH and temperature not specified in the publication
135
NADH
-
mutant enzyme R82D, pH and temperature not specified in the publication
137
NADH
-
mutant enzyme R197E
32
NADPH
mutant enzyme S188A
42
NADPH
mutant enzyme T33A/S188A
52
NADPH
mutant enzyme T33A
61
NADPH
native enzyme A3-GAPDH
161
NADPH
-
mutant enzyme K128E
196
NADPH
-
mutant enzyme R82A, pH and temperature not specified in the publication
203
NADPH
-
mutant enzyme R82D, pH and temperature not specified in the publication
210
NADPH
-
mutant enzyme S195A, pH and temperature not specified in the publication
220
NADPH
-
mutant enzyme K128A
220
NADPH
-
mutant enzyme R197E
251
NADPH
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase
392
NADPH
-
mutant enzyme R197A
430
NADPH
-
wild-type enzyme
430
NADPH
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase
430
NADPH
-
wild type enzyme, pH and temperature not specified in the publication
additional information
additional information
-
catalytic rate constant 238 s-1, control
-
additional information
additional information
-
catalytic rate constant 289 s-1, 3 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex
-
additional information
additional information
-
catalytic rate constant 316 s-1, 160 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex
-
additional information
additional information
-
catalytic rate constant 330 s-1, 10 microM thioredoxin, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex
-
additional information
additional information
-
catalytic rate constant 390 s-1, 10 microM thioredoxin, 3 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex
-
additional information
additional information
-
catalytic rate constant 462 s-1, 10 microM thioredoxin, 160 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex
-