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Literature summary for 1.2.1.13 extracted from

  • Graciet, E.; Lebreton, S.; Camadro, J.M.; Gontero, B.
    Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for confromation changes upon association with the small protein CP12 (2003), Eur. J. Biochem., 270, 129-136.
    View publication on PubMed
Search for more literature for 1.2.1.13

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information no Michaelis-Menten kinetics with 1,3-bisphosphoglycerate Chlamydomonas reinhardtii
0.018
-
 NADPH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
0.028
-
 NADPH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
0.12
-
 NADH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
0.136
-
 NADH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36854
-
 4 * 36854, native A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
37072
-
 4 * 37072, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
152000
-
 native A4 glyceraldehyde 3-phosphate dehydrogenase in complex with the small protein CP12, gel filtration Chlamydomonas reinhardtii
155000
-
 recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
3-phospho-D-glyceroyl phosphate + NADPH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36854, native A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
tetramer 4 * 37072, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
A4 glyceraldehyde 3-phosphate dehydrogenase
-
 Chlamydomonas reinhardtii
GAPDH
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH Chlamydomonas reinhardtii
41
-
 NADH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
88
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH Chlamydomonas reinhardtii
104
-
 NADH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
223
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH Chlamydomonas reinhardtii
251
-
 NADPH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
419
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH Chlamydomonas reinhardtii
430
-
 NADPH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 native enzyme, reaction with NADPH Chlamydomonas reinhardtii
7.8
-
 native and recombinant enzyme, reaction with NADH Chlamydomonas reinhardtii
7.9
-
 recombinant enzyme, reaction with NADPH Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADH greater specificity for NADPH than for NADH Chlamydomonas reinhardtii
NADPH greater specificity for NADPH than for NADH Chlamydomonas reinhardtii