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Literature summary for 1.2.1.13 extracted from

  • Erales, J.; Mekhalfi, M.; Woudstra, M.; Gontero, B.
    Molecular mechanism of NADPH-glyceraldehyde-3-phosphate dehydrogenase regulation through the C-terminus of CP12 in Chlamydomonas reinhardtii (2011), Biochemistry, 50, 2881-2888.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

Protein Variants

Protein Variants Comment Organism
R190A the catalytic constant, kcat, of the mutant in the presence of NADH decreases 10fold while the Km for NADH decreases 12fold. The mutant shows no activity with NADPH Chlamydomonas reinhardtii
R82A the mutation leads to a 10fold increase in the Km for NADPH but does not affect the kinetics of NADH Chlamydomonas reinhardtii
R82D the mutation leads to a 10fold increase in the Km for NADPH but does not affect the kinetics of NADH Chlamydomonas reinhardtii
S195A the mutation has no effect on the affinity of the enzyme for NADPH and its affinity for NADH and for BPGA in the presence of NADH is reduced Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
CP-12
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH mutant enzyme R190A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.028
-
NADPH wild type enzyme, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.028
-
NADPH mutant enzyme S195A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.102
-
NADH mutant enzyme R82D, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.12
-
NADH wild type enzyme, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.157
-
NADH mutant enzyme R82A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.202
-
NADPH mutant enzyme R82D, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.3
-
NADPH mutant enzyme R82A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
0.872
-
NADH mutant enzyme S195A, pH and temperature not specified in the publication Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADPH + H+ Chlamydomonas reinhardtii
-
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Trisacryl column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH + H+
-
Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
r
3-phospho-D-glyceroyl phosphate + NADPH + H+
-
Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r

Synonyms

Synonyms Comment Organism
GAPDH
-
Chlamydomonas reinhardtii
NADPH-glyceraldehyde-3-phosphate dehydrogenase
-
Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
NADH mutant enzyme R190A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
71
-
NADH mutant enzyme R82A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
104
-
NADH wild type enzyme, pH and temperature not specified in the publication Chlamydomonas reinhardtii
119
-
NADH mutant enzyme S195A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
135
-
NADH mutant enzyme R82D, pH and temperature not specified in the publication Chlamydomonas reinhardtii
196
-
NADPH mutant enzyme R82A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
203
-
NADPH mutant enzyme R82D, pH and temperature not specified in the publication Chlamydomonas reinhardtii
210
-
NADPH mutant enzyme S195A, pH and temperature not specified in the publication Chlamydomonas reinhardtii
430
-
NADPH wild type enzyme, pH and temperature not specified in the publication Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADH
-
Chlamydomonas reinhardtii
NADPH preferred cofactor Chlamydomonas reinhardtii