Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
R190A | the catalytic constant, kcat, of the mutant in the presence of NADH decreases 10fold while the Km for NADH decreases 12fold. The mutant shows no activity with NADPH | Chlamydomonas reinhardtii |
R82A | the mutation leads to a 10fold increase in the Km for NADPH but does not affect the kinetics of NADH | Chlamydomonas reinhardtii |
R82D | the mutation leads to a 10fold increase in the Km for NADPH but does not affect the kinetics of NADH | Chlamydomonas reinhardtii |
S195A | the mutation has no effect on the affinity of the enzyme for NADPH and its affinity for NADH and for BPGA in the presence of NADH is reduced | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CP-12 | - |
Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | mutant enzyme R190A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.028 | - |
NADPH | wild type enzyme, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.028 | - |
NADPH | mutant enzyme S195A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.102 | - |
NADH | mutant enzyme R82D, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.12 | - |
NADH | wild type enzyme, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.157 | - |
NADH | mutant enzyme R82A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.202 | - |
NADPH | mutant enzyme R82D, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.3 | - |
NADPH | mutant enzyme R82A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.872 | - |
NADH | mutant enzyme S195A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADPH + H+ | Chlamydomonas reinhardtii | - |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Trisacryl column chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Chlamydomonas reinhardtii | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
Chlamydomonas reinhardtii | D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Chlamydomonas reinhardtii |
NADPH-glyceraldehyde-3-phosphate dehydrogenase | - |
Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.4 | - |
NADH | mutant enzyme R190A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
71 | - |
NADH | mutant enzyme R82A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
104 | - |
NADH | wild type enzyme, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
119 | - |
NADH | mutant enzyme S195A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
135 | - |
NADH | mutant enzyme R82D, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
196 | - |
NADPH | mutant enzyme R82A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
203 | - |
NADPH | mutant enzyme R82D, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
210 | - |
NADPH | mutant enzyme S195A, pH and temperature not specified in the publication | Chlamydomonas reinhardtii | |
430 | - |
NADPH | wild type enzyme, pH and temperature not specified in the publication | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Chlamydomonas reinhardtii | |
NADPH | preferred cofactor | Chlamydomonas reinhardtii |