Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloroplast protein CP12 | the chloroplast protein CP12 behaves as a negative regulator of GAPDH activity | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADPH + H+ | Chlamydomonas reinhardtii | - |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant and native GAPDH are purified to apparent homogeneity from Escherichia coli cells and Chlamydomonas reinhardtii, respectively | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
Chlamydomonas reinhardtii | D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Chlamydomonas reinhardtii |
GAPDH (A4) | - |
Chlamydomonas reinhardtii |
glyceraldehyde-3-phosphate dehydrogenase | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | catalytic rate constant 238 s-1, control | Chlamydomonas reinhardtii | |
additional information | - |
additional information | catalytic rate constant 289 s-1, 3 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex | Chlamydomonas reinhardtii | |
additional information | - |
additional information | catalytic rate constant 316 s-1, 160 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex | Chlamydomonas reinhardtii | |
additional information | - |
additional information | catalytic rate constant 330 s-1, 10 microM thioredoxin, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex | Chlamydomonas reinhardtii | |
additional information | - |
additional information | catalytic rate constant 390 s-1, 10 microM thioredoxin, 3 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex | Chlamydomonas reinhardtii | |
additional information | - |
additional information | catalytic rate constant 462 s-1, 10 microM thioredoxin, 160 microM 3-phospho-D-glyceroyl phosphate, NADPH-dependent activity of GAPDH in the GAPDH/CP12 complex | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
activity assay | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chlamydomonas reinhardtii |