1.14.19.8: pentalenolactone synthase

This is an abbreviated version!
For detailed information about pentalenolactone synthase, go to the full flat file.

Reaction

+ 2 reduced ferredoxin + 2 H+ =

+ 2 oxidized ferredoxin + 2 H2O

Synonyms

CYP161C2, EC 1.3.7.10, penM, pntM

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.19.8 pentalenolactone synthase

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Results	in table
65	AA Sequence
3	Cloned(Commentary)
4	Cofactor
1	Crystallization (Commentary)
4	Expression
2	General Information
7	kcat/KM [mM/s]
10	KM Value [mM]
1	Metals/Ions
4	Natural Substrates/ Products (Substrates)
20	Organism
4	Pathway
9	PDB ID
1	pH Optimum
8	Protein Variants
3	Purification (Commentary)
2	Reaction
10	Reference
11	Substrates and Products (Substrate)
5	Subunits
12	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
9	Turnover Number [1/s]

Purification

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Purification on EC 1.14.19.8 - pentalenolactone synthase

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Ni2+-NTA resin column chromatography

2 entries

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, and gel filtration of the eluate, followed by ultrafiltration

Streptomyces arenae

E3VWI3

745166