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Literature summary for 1.14.19.8 extracted from

  • Zhu, D.; Seo, M.J.; Ikeda, H.; Cane, D.E.
    Genome mining in Streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone (2011), J. Am. Chem. Soc., 133, 2128-2131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces exfoliatus
expressed in Escherichia coli BL21(DE3) cells Streptomyces arenae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces exfoliatus
0.43
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces arenae

Organism

Organism UniProt Comment Textmining
Streptomyces arenae
-
-
-
Streptomyces arenae TUE469
-
-
-
Streptomyces exfoliatus
-
-
-
Streptomyces exfoliatus UC5319
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography Streptomyces exfoliatus
Ni2+-NTA resin column chromatography Streptomyces arenae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces exfoliatus pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces arenae pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces exfoliatus UC5319 pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces arenae TUE469 pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir

Subunits

Subunits Comment Organism
monomer
-
 Streptomyces exfoliatus
monomer
-
 Streptomyces arenae

Synonyms

Synonyms Comment Organism
penM
-
 Streptomyces exfoliatus
pntM
-
 Streptomyces arenae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.8
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces arenae
10.5
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces exfoliatus

Cofactor

Cofactor Comment Organism Structure
NADPH required for activity Streptomyces exfoliatus
NADPH required for activity Streptomyces arenae