1.14.19.8: pentalenolactone synthase

This is an abbreviated version!
For detailed information about pentalenolactone synthase, go to the full flat file.

Reaction

Synonyms

CYP161C2, EC 1.3.7.10, penM, pntM

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

                1.14.19.8 pentalenolactone synthase

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Results	in table
65	AA Sequence
3	Cloned(Commentary)
4	Cofactor
1	Crystallization (Commentary)
4	Expression
2	General Information
7	kcat/KM [mM/s]
10	KM Value [mM]
1	Metals/Ions
4	Natural Substrates/ Products (Substrates)
20	Organism
4	Pathway
9	PDB ID
1	pH Optimum
8	Protein Variants
3	Purification (Commentary)
2	Reaction
10	Reference
11	Substrates and Products (Substrate)
5	Subunits
12	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
9	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.19.8 - pentalenolactone synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified wild-type enzyme enzyme PntM substrate-free, and PntM with bound substrate pentalenolactone F, product pentalenolactone, and substrate analogue 6,7-dihydropentalenolactone, and recombinant PntM F232L, M77S, M81A, M81C, and M81C-BME mutants with bound pentalenolactone F, sitting drop vapor diffusion method, mixing of 0.001 ml of 16.8 mg/mL protein in 10 mM Tris-HCl, 15 mM NaCl, and 10% glycerol, with 0.001 ml of reservoir solution containing 1.2 M sodium citrate, 10% glycerol, and 100 mM bicine, pH 9.0, 15°C, followed by cross-microseeding technique, X-ray diffraction structure determination and analysis at 1.54 A and 2.06-2.12 A resolution, respectively, molecular replacement using the crystal structure of polyene macrolide epoxidase PimD, PDB ID 2X9P, as search model

Streptomyces arenae

E3VWI3

745166