Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.32: 17alpha-hydroxyprogesterone deacetylase

This is an abbreviated version!
For detailed information about 17alpha-hydroxyprogesterone deacetylase, go to the full flat file.

Word Map on EC 1.14.14.32

Reaction

17alpha-hydroxypregnenolone
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
3beta-hydroxyandrost-5-en-17-one
 +
acetate
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

17,20 lyase, 17-20 lyase, 17-alpha-hydroxylase/C17-20 lyase, 17-hydroxylase/C17,20-lyase, 17alpha-hydroxylase, 17alpha-hydroxylase-C(17,20)-lyase, 17alpha-hydroxylase-C17,20-lyase, 17alpha-hydroxylase/17,20 lyase, 17alpha-hydroxylase/17,20-lyase, 17alpha-hydroxylase/17,20-lyase cytochrome P450, 17alpha-hydroxylase/C(17,20)-lyase, 17alpha-hydroxylase/C17,20-lyase, 17alpha-hydroxyprogesterone aldolase, 17alpha-hydroxyprogesterone aldolase/17,20-lyase, 17alpha-lyase, Adrenal 17,20-lyase, aldolase, 17alpha-hydroxyprogesterone, C-17,20 lyase, C-17/C-20 lyase, C17(20) lyase, C17,20 lyase, C17,20-lyase, CYP17, CYP17A1, cytochrome P450 17, cytochrome P450 17A1, cytochrome P450 17alpha-hydroxylase/17,20-lyase, cytochrome p450 17alpha-hydroxylase/C(17,20)-lyase, cytochrome P450c17, EC 4.1.2.30, P-45017alpha, P450 17A1, P45017alpha, P450c17, P450c17-I, steroid 17alphahydroxylase/17,20-lyase, Steroid C17(20) lyase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.32 17alpha-hydroxyprogesterone deacetylase

Engineering

Engineering on EC 1.14.14.32 - 17alpha-hydroxyprogesterone deacetylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A355T
-
shows a complete loss of enzymatic activity. Is expressed in transfected COS-1 cells in a fashion comparable to the wild-type protein. Less severely affected patient with ambiguous genitalia
D298V
mutant without heme-binding properties
DELTAF54
-
partially reduces enzymatic activities
E305G
naturally occurring P450 17A1 mutation, impairs 17,20-lyase activity. Cytochrome b5 fails to rescue the poor coupling with 17-hydroxypregnenolone
G111D
mutant without heme-binding properties
G111S
-
shows a complete loss of enzymatic activity. Is expressed in transfected COS-1 cells in a fashion comparable to the wild-type protein. Leads to complete lack of masculinization in the patient
G301I
mutant without heme-binding properties
G90D
the mutation results in loss of the 17alpha-hydroxylase and 17,20-lyase activities
H373N
-
mutation almost completely eliminates enzymatic activities
I332T
-
retains some residual 17,20-lyase activity (10%). Is expressed in transfected COS-1 cells in a fashion comparable to the wild-type protein. Less severely affected patient with ambiguous genitalia
P342T
the mutant shows 20% of wild type activity
Q461stop
naturally occuring mutation, inactive mutant
R347H
R358Q
R358X
-
mutant, CGA to TGA, alteration introduces premature stop codon, inactive protein
R440H
-
shows a complete loss of enzymatic activity. Leads to complete lack of masculinization in the patient
R449A
R496C
naturally occuring mutation, the mutant has low 17alpha-hydroxylase and 17,20-lyase activities
R96W
the mutation results in loss of the 17alpha-hydroxylase and 17,20-lyase activities
S106P
the mutation results in loss of the 17alpha-hydroxylase and 17,20-lyase activities
T306A
T64S
the mutant shows 15% of wild type activity
Y329DEL-SUB
-
mutant, TAC to AA, alteration introduces premature stop codon, inactive protein
additional information