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1.14.14.102: N-methylcoclaurine 3'-monooxygenase

This is an abbreviated version!
For detailed information about N-methylcoclaurine 3'-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.102

Reaction

(S)-N-methylcoclaurine
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
(S)-3'-hydroxy-N-methylcoclaurine
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

(S)-methylcoclaurine-3'-hydroxylase, (S)-N-methylcoclaurine 3'-hydroxylase, (S)-N-methylcoclaurine-3'-hydroxylase, (S)-N-methylcoclaurine-3-hydroxylase, CYP80B, CYP80B1, CYP80B3, Cytochrome P450 80B1, Cytochrome P450 80B2, EC 1.14.13.71, LOC104585940, N-methylcoclaurine 3'-hydroxylase, NMCH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.102 N-methylcoclaurine 3'-monooxygenase

Cloned

Cloned on EC 1.14.14.102 - N-methylcoclaurine 3'-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae and Spodoptera frugiperda
-
gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine
-
gene LOC104585940 encoding isozyme 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
heterologous expression
-
PDR-amplification