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Literature summary for 1.14.14.102 extracted from

  • Yang, M.; Zhu, L.; Li, L.; Li, J.; Xu, L.; Fen, J.; Liu, Y.
    Digital gene expression analysis provides insight into the transcript profile of the genes involved in aporphine alkaloid biosynthesis in lotus (Nelumbo nucifera) (2017), Front. Plant Sci., 8, 80 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene LOC104585940 encoding isozyme 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Nelumbo nucifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 Nelumbo nucifera
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 (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Nelumbo nucifera A0A1U7Z269 isozyme 2; 2 isozymes, 2 cultivars
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
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 Nelumbo nucifera
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nelumbo nucifera (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
(S)-N-methylcoclaurine-3-hydroxylase
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 Nelumbo nucifera
CYP80B
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 Nelumbo nucifera
LOC104585940 locus name Nelumbo nucifera
N-methylcoclaurine 3'-hydroxylase
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 Nelumbo nucifera
NMCH
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 Nelumbo nucifera

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Nelumbo nucifera
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Nelumbo nucifera

General Information

General Information Comment Organism
metabolism the enzyme is involved in the common pathway of benzylisoquinoline alkaloid biosynthesis and the aporphine alkaloid bisynthesis pathway in lotus, overview. A series of enzymes, norcoclaurine 6-O-methyltransferase (6OMT), coclaurineN-methyltransferase (CNMT), (S)-N-methylcoclaurine-3-hydroxylase (NMCH), and 3-hydroxy-N-methylcoclaurine 4'-O-methyltransferase(4'OMT), exhibit catalytic activity to yield the central branchpoint intermediate (S)-reticuline Nelumbo nucifera
physiological function the expression of isozyme 1 is low in the two cultivars, so it may have little effect on alkaloid contents. The expression of CYP80B, NNU08355, has a positive effect on aporphine alkaloids content in lotus, but exhibits phenotypic lag, which might be because of NMCH which acts early in the benzylisoquinoline alkaloid biosynthetic pathway Nelumbo nucifera