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1.14.13.187: L-evernosamine nitrososynthase

This is an abbreviated version!
For detailed information about L-evernosamine nitrososynthase, go to the full flat file.

Word Map on EC 1.14.13.187

Reaction

dTDP-beta-L-evernosamine
+ 2 NADPH + 2 H+ + 2 O2 =
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose
 + 2 NADP+ + 3 H2O

Synonyms

DnmZ, nitrososynthase, ORF36

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.187 L-evernosamine nitrososynthase

Crystallization

Crystallization on EC 1.14.13.187 - L-evernosamine nitrososynthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 3.15 A resolution. Tetrameric enzyme that shares a fold with acyl-CoA dehydrogenases and class D flavin-containing monooxygenases, including the nitrososynthase KijD3Enzym displays an unusually open active site. Modeling of FAD and TDP-L-evernosamine into the active site
purified enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml protein solution containing 6 mg/ml of protein DnmZ with 0.001 ml crystallization solution containing 0.05 M glycine pH 9.5, 0.2 M ammonium sulfate, 10% w/v, and PEG 4000, and equilibration against 0.75 ml crystallization solution, 26°C, X-ray diffraction structure determination and analysis at 2.74-3.0 A resolution, molecular replacement using crystal structure PDB ID 3mxl as search model. Cocrystallization of DnmZ complexed with dTDP-L-epi-vancosamine, FAD, FMN or combinations of the amino sugar and flavin are unsuccessful