   1.14.13.187 | purified enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml protein solution containing 6 mg/ml of protein DnmZ with 0.001 ml crystallization solution containing 0.05 M glycine pH 9.5, 0.2 M ammonium sulfate, 10% w/v, and PEG 4000, and equilibration against 0.75 ml crystallization solution, 26°C, X-ray diffraction structure determination and analysis at 2.74-3.0 A resolution, molecular replacement using crystal structure PDB ID 3mxl as search model. Cocrystallization of DnmZ complexed with dTDP-L-epi-vancosamine, FAD, FMN or combinations of the amino sugar and flavin are unsuccessful |
