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1.14.13.172: salicylate 5-hydroxylase

This is an abbreviated version!
For detailed information about salicylate 5-hydroxylase, go to the full flat file.

Wordmap for 1.14.13.172

Word Map on EC 1.14.13.172 Wordmap for 1.14.13.172

Reaction

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salicylate
+
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NADH
+
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H+
+
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O2
=
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2,5-Dihydroxybenzoate
+
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NAD+
+
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H2O

Synonyms

nagG, NagGH, nagH, S5H, S5HR, SAL5H, SalABCD, SALD

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                EC 1.14.13.1721.14.13.172 salicylate 5-hydroxylase

Cloned

Cloned on EC 1.14.13.172 -

for references in articles please use BRENDA:EC1.14.13.172

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme S5H is encoded in the salABCD gene cluster, which locates on the chromosome rather than on a mobile plasmid, functional recombinant expression in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)/pLysS cells
expressed in Escherichia coli DH5alpha cells