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1.14.13.172: salicylate 5-hydroxylase

This is an abbreviated version!
For detailed information about salicylate 5-hydroxylase, go to the full flat file.

Word Map on EC 1.14.13.172

Reaction

salicylate
+
NADH
 +
H+
 +
O2
=
2,5-Dihydroxybenzoate
 +
NAD+
 +
H2O

Synonyms

nagG, NagGH, nagH, S5H, S5HR, SAL5H, SalABCD, SALD

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.172 salicylate 5-hydroxylase

General Information

General Information on EC 1.14.13.172 - salicylate 5-hydroxylase

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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
O52378; O52381; O52379; O52380
binding of salicylate and O2 is fast. Transfer of the Rieske electron required to form the gentisate product occurs through bonds over about 12 A and must also be very fast. The observed rate constant for this reaction is much slower than expected and sensitive to substrate type. A transient intermediate (lambdamax of 700 nm) with an EPR resonance at g 4.3 is observed after product is formed in the active site. Gentisate binds to the mononuclear iron via its C5-OH in the intermediate. Rereduction of the metal centers accelerates product release about 300fold
physiological function
enzyme S5H hydroxylates salicylate into gentisate and is involved in the degradation of aromatic compounds. SalD is an essential component of the S5H complex