Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)/pLysS cells | Ralstonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
Ralstonia sp. U2 | O52379 and O52380 | large oxygenase component and small oxagenase component | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.051 | - |
in 50 mM MES buffer, pH 6.4, at 30°C | Ralstonia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone | Ralstonia sp. | ? | - |
? | |
additional information | no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone | Ralstonia sp. U2 | ? | - |
? | |
salicylate + NADH + H+ + O2 | - |
Ralstonia sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
salicylate + NADH + H+ + O2 | - |
Ralstonia sp. U2 | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NagGH | - |
Ralstonia sp. |
S5H | a three-component system consisting of NagGH (the oxygenase components), NagAa (ferredoxin reductase), and NagAb (ferredoxin) | Ralstonia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Ralstonia sp. |