1.14.12.12: naphthalene 1,2-dioxygenase

This is an abbreviated version!
For detailed information about naphthalene 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.12

Reaction

Naphthalene
+
NADH
+
H+
+
O2
=
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
+
NAD+

Synonyms

NAG, NAHA, nahAc, naphthalene 1,2-dioxygenase, naphthalene dioxygenase, naphthalene oxygenase, NDO, oxygenase, naphthalene di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.12 naphthalene 1,2-dioxygenase

Purification

Purification on EC 1.14.12.12 - naphthalene 1,2-dioxygenase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
affinity chromatography separates the reductase from the two other enzyme components; purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP
-
ammonium sulfate, Sephadex G-75, DEAE-cellulose
-
Blue Sepharose, Q-Sepharose, Superose 12, purification of component B, the terminal oxygenase ISPNAR
-
component B i.e. the terminal oxygenase ISPNAP, DEAE-Sephadex, DEAE-cellulose, octyl-Sepharose, presence of 10% ethanol and 10% glycerol is required to maintain stability
-
component B i.e. the terminal oxygenase ISPNAP, DEAE-Sephadex, DEAE-cellulose, octyl-Sepharose, presence of 10% ethanol and 10% glycerol is required to maintain stability; ferredoxinNAP reductase, Blue Sepharose, DEAE-cellulose; purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP
-
DEAE-cellulose DE52 column chromatography, immobilized metal affinity chromatography and DEAE cellulose column chromatography
-
ferredoxinNAP reductase, Blue Sepharose, DEAE-cellulose
-
metal affinity chromatography
-
Ni-NTA column chromatography
-
Q Sepharose column chromatography, octyl Sepharose column chromatography, and hydroxyapatite ion-exchange column chromatography
Q-Sepharose FF column chromatography and 1 hydroxyapatite ion-exchange column chromatography
-