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Literature summary for 1.14.12.12 extracted from

  • Larkin, M.J.; Allen, C.C.R.; Kulakov, L.A.; Lipscomb, D.A.
    Purification and characterization of a novel naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038 (1999), J. Bacteriol., 181, 6200-6204.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.12

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ terminal dioxygenase ISPNAR may be a Rieske-type iron-sulfur protein containing 2.4 g-atoms of iron and 2.1 g-atoms of sulfur per alpha,beta subunit Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.
55000
-
 alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.
155000
-
 terminal dioxygenase ISPNAR, gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. NCIMB12038
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose, Q-Sepharose, Superose 12, purification of component B, the terminal oxygenase ISPNAR Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
 activity of terminal dioxygenase ISPNAR Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
naphthalene + NAD(P)H + O2
-
 Rhodococcus sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
 ?
naphthalene + NAD(P)H + O2
-
 Rhodococcus sp. NCIMB12038 cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
More X-ray structure data from Pseudomonas sp. 9861-4 suggest an alpha3,beta3 hexameric structure Rhodococcus sp.
tetramer alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD stimulates activity of terminal oxygenase ISPNAR Rhodococcus sp.
FMN stimulates activity of terminal oxygenase ISPNAR Rhodococcus sp.
NADH
-
 Rhodococcus sp.
NADPH
-
 Rhodococcus sp.