Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
F352V | mutation changes the product regioselectivities for naphthalene, phenanthrene, and biphenyl | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. NCIB 9816-4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose FF column chromatography and 1 hydroxyapatite ion-exchange column chromatography | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitrotoluene + NADH + O2 | - |
Pseudomonas sp. | 4-nitrobenzyl alcohol + NAD+ | - |
? | |
3-nitrotoluene + NADH + O2 | - |
Pseudomonas sp. NCIB 9816-4 | 4-nitrobenzyl alcohol + NAD+ | - |
? | |
anthracene + NADH + O2 | - |
Pseudomonas sp. | (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+ | - |
? | |
anthracene + NADH + O2 | - |
Pseudomonas sp. NCIB 9816-4 | (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+ | - |
? | |
biphenyl + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ | - |
? | |
biphenyl + NADH + O2 | - |
Pseudomonas sp. NCIB 9816-4 | ? + NAD+ | - |
? | |
naphthalene + NADH + O2 | - |
Pseudomonas sp. | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
naphthalene + NADH + O2 | - |
Pseudomonas sp. NCIB 9816-4 | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
phenanthrene + NADH + O2 | - |
Pseudomonas sp. | (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+ | - |
? | |
phenanthrene + NADH + O2 | - |
Pseudomonas sp. NCIB 9816-4 | (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDO | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. |