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Literature summary for 1.14.12.12 extracted from

  • Ferraro, D.J.; Okerlund, A.L.; Mowers, J.C.; Ramaswamy, S.
    Structural basis for regioselectivity and stereoselectivity of product formation by naphthalene 1,2-dioxygenase (2006), J. Bacteriol., 188, 6986-6994.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
F352V mutation changes the product regioselectivities for naphthalene, phenanthrene, and biphenyl Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. NCIB 9816-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose FF column chromatography and 1 hydroxyapatite ion-exchange column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitrotoluene + NADH + O2
-
 Pseudomonas sp. 4-nitrobenzyl alcohol + NAD+
-
 ?
3-nitrotoluene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 4-nitrobenzyl alcohol + NAD+
-
 ?
anthracene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+
-
 ?
anthracene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydroanthracene-1,2-diol + (1S,2R)-1,2-dihydroanthracene-1,2-diol + NAD+
-
 ?
biphenyl + NADH + O2
-
 Pseudomonas sp. ? + NAD+
-
 ?
biphenyl + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 ? + NAD+
-
 ?
naphthalene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
naphthalene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
phenanthrene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 ?
phenanthrene + NADH + O2
-
 Pseudomonas sp. NCIB 9816-4 (1R,2S)-1,2-dihydrophenanthrene-1,2-diol + (1S,2R)-1,2-dihydrophenanthrene-1,2-diol + (3R,4S)-3,4-dihydrophenanthrene-3,4-diol + (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
NDO
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas sp.