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(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid + H2O
?
-
-
-
-
?
(R)-[2-(benzoylamino)propionylsulfanyl]acetic acid + H2O
?
-
-
-
?
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine + H2O
2-(N-glycyl-L-cysteinyl)acetyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-cysteaminyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate + H2O
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanine + D-thiolactate
-
very efficient substrate for R61 DD-peptidase
-
-
?
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O
(2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
(2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate + H2O
?
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate + H2O
?
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid + H2O
?
-
-
-
-
?
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid + H2O
?
-
-
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Ala
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln + Gly-L-Ala
Ac2-L-Lys-D-Ala-Gly-L-Ala + ?
-
transpeptidation
-
-
r
Ac2-L-Lys-D-Ala-Gly-L-Leu
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Leu + D-alanine
Ac2-L-Lys-D-Ala-D-Ala + ?
-
transpeptidation
-
-
r
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala-D-Ala + H2O
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala + D-Ala
-
-
-
-
?
acetyl-L-Lys-D-Ala-D-Ala + H2O
acetyl-L-Lys-D-Ala + D-Ala
benzoyl-D-Ala-thioglycolate + H2O
?
-
-
?
benzoyl-Gly-thioglycolate + H2O
?
-
-
?
benzoyl-Gly-thiolactate + H2O
?
-
-
?
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-Ala-D-Ala
?
-
-
-
?
D-Ala-D-Ala + H2O
2 D-Ala
D-Ala-D-Ala + H2O
D-Ala + D-Ala
D-Ala-D-Phe + H2O
D-Ala + D-Phe
-
-
-
-
?
D-Ala-D-Trp + H2O
D-Ala + D-Trp
-
-
-
-
?
D-Ala-D-Tyr + H2O
D-Ala + D-Tyr
-
-
-
-
?
D-amino acid amide + H2O
D-amino acid + ammonia
-
-
-
?
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala-D-Ala + H2O
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala + D-Ala
-
-
-
-
?
Gly-gamma-L-Glu-D-Ala-D-Ala + H2O
Gly-gamma-L-Glu-D-Ala + D-Ala
-
-
-
?
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala + H2O
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala + D-Ala
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-alanine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-leucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-leucine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-norleucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-norleucine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + glycine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-alanine + H2O
?
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-leucine + H2O
?
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-phenylalanine + H2O
?
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
-
-
-
-
?
hippuryl-mercaptoacetic acid
hippuric acid + mercaptoacetate
-
-
-
-
?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O
?
-
only substrate of penicillin-binding protein 5, but not of penicillin-binding protein 6
-
-
?
L-Lys-D-Ala-D-Ala + H2O
L-Lys-D-Ala + D-Ala
-
-
-
-
?
N,N'-diacetyl-L-Lys-D-Ala-D-Ala + H2O
?
Streptomyces pneumoniae
-
-
-
-
?
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
-
-
-
-
?
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
-
-
-
?
N-acetyl-D-Ala-D-Ala + H2O
?
-
-
-
?
N-acetyl-D-Ala-D-Ala + H2O
N-acetyl-D-Ala + D-Ala
-
-
-
?
N-acetyl-L-Lys-D-Ala-D-Ala + H2O
N-acetyl-L-Lys-D-Ala + D-Ala
-
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + D-alanine
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanine + D-alanine
-
-
-
?
N-alpha-acetyl-L-Lys-D-Ala-thiolactate + H2O
?
-
-
?
N-benzoyl-D-Ala + H2O
benzoate + D-Ala
-
substrate binding structure
-
-
?
N-benzoyl-D-Ala-thio-D-lactate
?
-
-
-
?
N-benzoyl-D-Ala-thioglycolate
?
-
-
-
?
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine + H2O
?
-
-
-
-
?
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
?
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
N,N'-diacetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-lactate + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-lactate
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate + H2O
?
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
-
substrate in DD-carboxypeptidase activity assay
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
D-alanine + Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanine
Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Neta-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
-
substrate for assay of DD-carboxypeptidase activity
-
-
?
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala-D-Ala + H2O
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala + D-Ala
-
-
-
-
?
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanyl-D-alanine + H2O
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanine + D-alanine
-
-
-
-
?
Nalpha-acetyl-L-Lys-D-Ala-D-Ala + H2O
?
substrate is used to detect the hydrolyzing activity of PBP4a
-
-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
-
-
?
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala + D-Ala
-
-
-
?
phenylacetyl-D-Ala-thioglycolate + H2O
?
-
-
?
phenylacetyl-D-Ala-thiolactate + H2O
?
-
-
?
S2a + D-alanine
?
-
-
-
-
?
S2a + D-leucine
?
-
-
-
-
?
S2a + D-phenylalanine
?
-
-
-
-
?
S2a + Gly-Gly
?
-
-
-
-
?
S2a + Gly-Gly-Gly
?
-
-
-
-
?
S2a + Gly-L-Ala
?
-
-
-
-
?
S2a + Gly-L-Gln
?
-
-
-
-
?
S2a + Gly-L-Leu
?
-
one of the most efficient acceptors
-
-
?
S2d thiolester
?
-
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala + D-Ala
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
X-D-Ala-D-Ala + H2O
?
-
-
-
-
?
additional information
?
-
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O
(2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate
no reaction with (S)-enantiomer
substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O
(2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate
no reaction with (S)-enantiomer
substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
(2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate
no reaction with (S)-enantiomer
substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
(2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate
no reaction with (S)-enantiomer
substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate + H2O
?
-
-
-
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate + H2O
?
-
-
-
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate + H2O
?
-
-
-
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate + H2O
?
-
-
-
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate + H2O
?
-
-
-
-
?
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate + H2O
?
-
-
-
-
?
acetyl-L-Lys-D-Ala-D-Ala + H2O
acetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
acetyl-L-Lys-D-Ala-D-Ala + H2O
acetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
D-Ala-D-Ala + H2O
2 D-Ala
-
-
-
?
D-Ala-D-Ala + H2O
2 D-Ala
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala + D-Ala
-
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala + D-Ala
-
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala + D-Ala
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
ir
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
ir
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
ir
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
acceptors in transpeptidase reaction
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
D-alanine + Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanine
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
D-alanine + Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanine
-
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala
-
i.e. UDP-N-acetylmuraminoyl-pentapeptide, release of D-alanine from UDPMurNAc-pentapeptide substrate
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala
-
i.e. UDP-N-acetylmuraminoyl-pentapeptide, release of D-alanine from UDPMurNAc-pentapeptide substrate
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala + D-Ala
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala + D-Ala
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
28888, 36276, 36277, 36278, 36279, 36280, 36281, 36282, 36283, 36284, 36285, 36286, 36287, 36289, 36290, 36291, 36297, 36301, 36302, 36303, 36304 -
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
28888, 36276, 36277, 36278, 36279, 36280, 36281, 36282, 36283, 36284, 36285, 36286, 36287, 36289, 36290, 36291, 36297, 36301, 36302, 36303, 36304 -
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
-
-
-
?
additional information
?
-
-
requirement for D-Ala in P1 position
-
-
?
additional information
?
-
-
the enzyme cleaves the C-terminal D-Ala5 of stem pentapeptides and forms the essential tetrapeptide substrate of a beta-lactam-insensitive L,D-transpeptidase
-
-
?
additional information
?
-
-
bifunctional enzyme catalyzing the reactions of D-Ala-D-Ala-carboxypeptidase EC 3.4.16.4 and D-Ala-D-Ala-dipeptidase EC 3.4.13.22
-
-
?
additional information
?
-
-
not D-Ala-D-lactate
-
-
?
additional information
?
-
-
penicillin-binding protein
-
?
additional information
?
-
-
penicillin-binding protein
-
?
additional information
?
-
-
forms a covalent acyl-enzyme complex with beta-lactam antibiotics, high rate of decylation of the acyl-enzyme complex. Direct role for the SXN motif in deacylation of the acyl-enzyme complex. Functioning of this motif is modulated by the 74-90 loop. Ser86 and Ser87 are important for D-alanine carboxypeptidase activity
-
?
additional information
?
-
-
the enzyme forms a covalent acyl-enzyme complex with beta-lactam antibiotics
-
?
additional information
?
-
-
enzyme removes the terminal D-alanine from the pentapeptide side chains of muramic acid in peptidoglycan
-
-
?
additional information
?
-
on substrates mimicking peptidoglycan precursors, isoform VanYn shows D,D-carboxypeptidase and D,D-dipeptidase activity, but lacks D,D-carboxyesterase ability on D-Ala-D-Lac-terminating peptides
-
-
?
additional information
?
-
on substrates mimicking peptidoglycan precursors, isoform VanYn shows D,D-carboxypeptidase and D,D-dipeptidase activity, but lacks D,D-carboxyesterase ability on D-Ala-D-Lac-terminating peptides
-
-
?
additional information
?
-
-
low molecular mass PBPs can exert carboxypeptidase and/or endopeptidase activities and play auxiliary roles in the peptidoglycan metabolism process
-
-
?
additional information
?
-
-
the serine protease catalyzes glycosyl transfer, i.e. polymerization of glycan chains, and transpeptidation, i.e. cross-linking of stem peptides, which are essential for peptidoglycan stability in bacterial cell wall synthesis, and for the cell division process, overview
-
-
?
additional information
?
-
-
influence of substrate conformation
-
-
?
additional information
?
-
-
requirement for D-Ala in P1 position
-
-
?
additional information
?
-
specific substrate binding to the peptidase induces a conformational change in the active site that places Ser62 in an optimal position for catalysis. This activated conformation relaxes as the reaction proceeds
-
?
additional information
?
-
the enzyme is responsible for the final peptidoglycan cross-linking step in bacterial cell wall biosynthesis
-
?
additional information
additional information
-
-
the glycosyl transferase of PBP1b catalyzes the assembly of lipid-transported N-acetylglucosaminyl-beta-1,4-N-acetylmuramoyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala units
linear peptidoglycan chains
-
?
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(2,3)-alpha-methylene benzylpenicillin
-
modeling of enzyme-inhibitor complex
(2-phenylethyl)boronic acid
91% residual activity at 1 mM
(2R)-2-amino-7-oxo-7-[(4,4,4-trifluoro-3-oxobutan-2-yl)amino]heptanoic acid
i.e. D-alpha-aminopimelyl-(1,1,1-trifluoro-3-amino)butan-2-one
(3-aminophenyl)boronic acid
20% residual activity at 1 mM
(3-nitrophenyl)boronic acid
82% residual activity at 1 mM
(3H)benzylpenicillin
Streptomyces pneumoniae
-
-
(3R,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5R,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5S,6R)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
-
-
(3R,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5R,6S)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
-
-
(3S,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(4-phenoxyphenyl)boronic acid
98% residual activity at 1 mM
(5-acetylthiophen-2-yl)boronic acid
88% residual activity at 1 mM
(5-chlorothiophen-2-yl)boronic acid
91% residual activity at 1 mM
(5-cyanothiophen-2-yl)boronic acid
95% residual activity at 1 mM
(5-formylthiophen-2-yl)boronic acid
99% residual activity at 1 mM
(5R,6R)-6-[[(6S)-6-carboxy-6-(glycylamino)hexanoyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
-
-
(6R,7R)-3-[(acetyloxy)methyl]-7-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
1-benzothiophen-2-ylboronic acid
86% residual activity at 1 mM
2,1-benzoxaborol-1(3H)-ol
45% residual activity at 1 mM
2,3-dimercapto-1-propane-sulfonic acid
-
-
2,3-dimercapto-1-propanol
-
-
3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoic acid
38% residual activity at 0.5 mM
3-(acetylamino)-5-(dihydroxyboranyl)benzoic acid
50% residual activity at 1 mM
3-(benzoylamino)-5-(dihydroxyboranyl)benzoic acid
9% residual activity at 1 mM
3-(dihydroxyboranyl)-4-[(phenylacetyl)amino]benzoic acid
80% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(2,6-dimethoxybenzoyl)amino]benzoic acid
84% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(phenoxyacetyl)amino]benzoic acid
2% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(thiophen-2-ylacetyl)amino]benzoic acid
5% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(thiophen-2-ylcarbonyl)amino]benzoic acid
18% residual activity at 1 mM
3-(dihydroxyboryl)-5-(2-methoxybenzamido)benzoic acid
10% residual acvtivity at 1 mM
3-(dihydroxyboryl)-5-(2-phenylacetamido)benzoic acid
3% residual acvtivity at 1 mM
3-(dihydroxyboryl)benzoic acid
20% residual acvtivity at 1 mM
3-(N-glycyl-L-cysteinyl)-N-ethylpropionamide
-
-
3-(trifluoroacetyl)benzoic acid
60% residual activity at 1 mM
3-formylbenzoic acid
77% residual activity at 1 mM
4-(dihydroxyboranyl)benzoic acid
85% residual activity at 1 mM
4-chloromercuribenzoate
-
complete inhibition at 1 mM
4-[(phenylacetyl)amino]-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoic acid
81% residual activity at 1 mM
5-(dihydroxyboranyl)thiophene-2-carboxylic acid
57% residual activity at 1 mM
6-beta(alpha-N-acetyl-L-lysyl)-aminopenicillanic acid
-
-
6-beta(D-alpha-aminopimelyl)-aminopenicillanic acid
6-beta(D-alpha-aminosuberyl)-aminopenicillanic acid
6-beta(N-acetyl-L-alanyl-gamma-D-glutamyl-L-alanyl)-aminopenicillanic acid
-
-
7-(phenoxyacetamido)-3-desacetoxycephalosporamic acid
-
-
7-beta(alpha-N-acetyl-L-lysyl)-aminocephalosporanic acid
-
-
7-beta(D-alpha-aminopimelyl)-aminocephalosporanic acid
alpha-N-glycyl-epsilon-N-acetyl-L-lysine
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
arylakylidene imino-thiazolidin-4-ones 10
-
86% inhibition
arylakylidene imino-thiazolidin-4-ones 11
-
82% inhibition
arylakylidene imino-thiazolidin-4-ones 12
-
64% inhibition
arylakylidene imino-thiazolidin-4-ones 13
-
83% inhibition
arylakylidene imino-thiazolidin-4-ones 16
-
100% inhibition
arylakylidene imino-thiazolidin-4-ones 17
-
100% inhibition
arylakylidene imino-thiazolidin-4-ones 18
-
96% inhibition
arylakylidene imino-thiazolidin-4-ones 19
-
84% inhibition
arylakylidene imino-thiazolidin-4-ones 5
-
89% inhibition
arylakylidene imino-thiazolidin-4-ones 6
-
92% inhibition
arylakylidene imino-thiazolidin-4-ones 8
-
99% inhibition
arylakylidene imino-thiazolidin-4-ones 9
-
85% inhibition
arylalkylidene rhodanine derivative 1
-
88% inhibition
arylalkylidene rhodanine derivative 2
arylalkylidene rhodanine derivative 3
-
83% inhibition
arylalkylidene rhodanine derivative 4
-
95% inhibition
ayercillin
-
dipeptide-releasing activity is penicillin sensitive
biphenyl-4,4'-diyldiboronic acid
97% residual activity at 0.1 mM
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-boroAla-(-)-pinanediol
effective inhibitor of the D-alanine CPase activity of PBP5
cephalosporin C
classical non-specific beta-lactam
cetyltrimethylammonium bromide
-
-
D-Ala(P,O)D-Ala
-
acts as slow binding inhibitor
D-Ala(P,O)D-Phe
-
acts as slow binding inhibitor
D-alpha-phenylpropionate
-
-
Delta2-(and Delta3)-Deacetoxy-7-phenylacetamidocephalosporanates
-
-
-
diisopropyl fluorophosphate
-
1 mM, 28% inhibition
EDTA
4 mM, complete loss of activity. Ativity may be restored in presence of 8 mM Zn2+
epsilon-N-glycyl-alpha-N-acetyl-L-alanyl-D-isoglutaminyl-L-lysine
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
formaldehyde
-
1 mM, 53% inhibition
iodoacetamide
-
1 mM, 20% inhibition
Mercaptosuccinic acid
-
-
N,N-diacetyl-L-Lys-D-Ala-D-Ala
Streptomyces pneumoniae
-
inhibition of PBP3 by its own substrate above a ligand concentration of 15 mM
N-(glycyl)-D,L-alpha-aminocaprylic acid
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
N-benzoyl-beta-sultam
time-dependent, irreversible active site directed inhibitor, the rate of inactivation is first order with respect to beta-sultam concentration and second order rate constants show dependence on pH
N-Chlorosuccinimide
-
1 mM, 50% inhibition
N-p-methoxy-beta-sultam
least effective inhibitor
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala
-
substrate inhibition above 30 mM
NEM
-
1 mM, complete inhibition
Oxyimino-DELTA3-cephalosporins
-
p-hydroxymercuribenzoate
-
1 mM, complete inhibition
phenoxymethylpenicillin
-
-
phenyl glycyl-L-alpha-aminopimelyl-epsilon-(D-2-aminoethyl)phosphonate
-
thiophen-2-ylboronic acid
88% residual activity at 1 mM
Vancomycin
-
vancomycin derivatives inhibit the glycosyl transferase activity
[3-([[5-(dimethylamino)naphthalen-1-yl]sulfonyl]amino)phenyl]boronic acid
60% residual activity at 0.5 mM
[3-[(2,6-dimethoxybenzoyl)amino]-5-(methoxycarbonyl)thiophen-2-yl]boronic acid
98% residual activity at 1 mM
6-beta(D-alpha-aminopimelyl)-aminopenicillanic acid
-
-
6-beta(D-alpha-aminopimelyl)-aminopenicillanic acid
-
-
6-beta(D-alpha-aminosuberyl)-aminopenicillanic acid
-
-
6-beta(D-alpha-aminosuberyl)-aminopenicillanic acid
-
-
7-beta(D-alpha-aminopimelyl)-aminocephalosporanic acid
-
-
7-beta(D-alpha-aminopimelyl)-aminocephalosporanic acid
-
-
ampicillin
-
complete inhibition at 0.57 mM, 95% inhibition at 0.285 mM
arylalkylidene rhodanine derivative 2
-
95% inhibition
arylalkylidene rhodanine derivative 2
-
94% inhibition
arylalkylidene rhodanine derivative 2
-
75% inhibition
benzylpenicillin
-
ester-linked to serins
benzylpenicillin
-
IC50: 0.00002 mg/ml, inhibition of the enzyme influences production of peptidoglycan precursors internally
benzylpenicillin
-
80% inhibition at 0.1 mg/ml
benzylpenicillin
classical non-specific beta-lactam
beta-lactam
-
R39 has a high beta-lactam binding activity
beta-lactams
-
kinetic constants
-
beta-lactams
-
kinetic constants
-
Ca2+
1 mM, no residual activity
cephalosporin
-
-
cephalosporin
with glycyl-L-alpha-amino-epsilon-pimelyl side-chain, this beta lactam might be a better antibiotic than a non-specific counterpart
cephalothin
-
-
Methicillin
-
-
Mg2+
1 mM, no residual activity
Moenomycin
-
inhibits the glycosyl transferase activity
nocardicin A
-
-
Oxyimino-DELTA3-cephalosporins
-
syn- and anti-isomers
-
Oxyimino-DELTA3-cephalosporins
-
syn- and anti-isomers
-
penicillin
-
kinetic model of interaction
penicillin
-
binds covalently to Ser36, Bacillus subtilis, Bacillus stearothermophilus
penicillin
-
binds covalently to Ser36, Bacillus subtilis, Bacillus stearothermophilus
penicillin
-
the enzyme is sensitive to inhibition, penicillin-binding profile during development of spores, overview
penicillin
Streptomyces pneumoniae
-
-
penicillin
-
kinetic model of interaction
penicillin
having the glycyl-L-alpha-amino-epsilon-pimelyl side-chain of Streptomyces strain R61 peptidoglycan, this beta lactam might be the perfect antibiotic as compared to a non-specific counterpart
penicillin G
-
100% inhibition
penicillin G
-
100% inhibition
penicillin G
-
100% inhibition
phenylboronic acid
50% residual activity at 0.5 mM
phenylboronic acid
-
1 mM, 26% inhibition
piperacillin
-
-
sulfazecine
-
-
Zn2+
-
-
Zn2+
1 mM, no residual activity
additional information
-
no significant inhibition by arylalkylidene rhodanine derivative 1 and 3 respectively and arylakylidene imino-thiazolidin-4-ones 6, 8, 13, 16, 17 and 19 respectively
-
additional information
not inhibited by methyl 3-(dihydroxyboryl)-5-(2-phenylacetamido)benzoate
-
additional information
-
no inhibition: penicillin G, ampicillin, cephalosporin C
-
additional information
-
inhibition studies
-
additional information
-
no significant inhibition by arylakylidene imino-thiazolidin-4-ones 7, 14 and 15 respectively
-
additional information
-
no inhibition: EDTA
-
additional information
-
serine enzyme not inhibited by PMSF
-
additional information
-
no inhibition: EDTA
-
additional information
-
no inhibition: EDTA
-
additional information
-
inhibition studies
-
additional information
-
no significant inhibition by arylalkylidene rhodanine derivative 1, 3 and 4 respectively and arylakylidene imino-thiazolidin-4-ones 5 to 19
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid
-
-
0.86
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine
-
-
1.28
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
3
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine
-
-
0.004
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
-
-
0.48
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine
-
-
0.26
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
0.36 - 4.3
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
0.2 - 0.45
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
0.2 - 0.45
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
0.35 - 1.8
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
1.37
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid
-
-
0.76
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid
-
-
7.5
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala-D-Ala
-
-
8
acetyl-L-Lys-D-Ala-D-Ala
pH 7.4, 25°C
1.3 - 1.5
benzoyl-D-Ala-thioglycolate
0.8 - 3
benzoyl-Gly-thioglycolate
0.22 - 1
benzoyl-Gly-thiolactate
6.4
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-Ala-D-Ala
-
20
D-Ala-D-Ala
pH 7.4, 25°C
0.86
Gly-gamma-L-Glu-D-Ala-D-Ala
-
-
0.0079
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala
-
-
0.0079
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine
-
-
0.26
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
0.0041
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
0.12
hippuryl-mercaptoacetic acid
-
crystal and solution
1.38
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37°C
9.8
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine
-
-
3.5
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25°C
0.675 - 0.913
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
0.0178 - 0.401
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
0.4
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, wild-type enzyme
0.0079
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine
-
-
3.7
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25°C
12
Nalpha, Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
lysozyme releasable enzyme
0.1 - 14
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
2 - 3
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
1.45
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala-D-Ala
-
-
9.4 - 30
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
0.6
Nalpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
2.4 - 3
phenylacetyl-D-Ala-thioglycolate
1.2
phenylacetyl-D-Ala-thiolactate
1.4
UDP-N-acetylmuramyl-L-Ala-D-Glu-mesodiaminopimelyl-D-Ala-D-Ala
-
-
5 - 20
X-D-Ala-D-Ala
-
value depending on X
additional information
additional information
-
0.36
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
pH 7.5, 25°C
4.3
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
pH 7.5, 25°C
0.2
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
pH 7.5, 25°C
0.45
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
pH 7.5, 25°C
0.2
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
0.2
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
0.45
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
0.35
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
0.85
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
1.8
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
1.3
benzoyl-D-Ala-thioglycolate
pH 7.2, 37°C, mutant enzyme C98A
1.5
benzoyl-D-Ala-thioglycolate
pH 7.2, 37°C, wild-type enzyme
0.8
benzoyl-Gly-thioglycolate
pH 7.2, 37°C, mutant enzyme C98A
3
benzoyl-Gly-thioglycolate
above, pH 7.2, 37°C, wild-type enzyme
0.22
benzoyl-Gly-thiolactate
pH 7.2, 37°C, wild-type enzyme
0.3
benzoyl-Gly-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
1
benzoyl-Gly-thiolactate
pH 7.2, 37°C, mutant enzyme K38H
0.675
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant Q422S
0.678
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant Q422A
0.682
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant wild type enzyme
0.712
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant R361H
0.744
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant R361A
0.913
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant F160A
0.0178
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant R361H
0.0204
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
wild type enzyme
0.0344
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant F160A
0.116
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant Q422S
0.222
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant D155A
0.237
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant Q422A
0.401
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant R361A
0.1
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
54000 MW enzyme
0.35
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
above, pH 7.2, 37°C, mutant enzyme C98A
1.28
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
recombinant PBP 6, in 50 mM Tris-HCl, pH 8.5, at 37°C
3.8
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
-
6.2
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, wild-type enzyme
8.98
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37°C
14
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
-
14
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
-
2
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
3
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
above, pH 7.2, 37°C, wild-type enzyme
9.4
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, wild-type enzyme
30
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, mutant enzyme C98A
2.4
phenylacetyl-D-Ala-thioglycolate
pH 7.2, 37°C, mutant enzyme K38H
3
phenylacetyl-D-Ala-thioglycolate
above, pH 7.2, 37°C, mutant enzyme C98A
3
phenylacetyl-D-Ala-thioglycolate
above, pH 7.2, 37°C, wild-type enzyme
1.2
phenylacetyl-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
1.2
phenylacetyl-D-Ala-thiolactate
pH 7.2, 37°C, wild-type enzyme
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
the ratio of turnover number to Km-value for the substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala is 32/M*s for the wild-type enzyme, 3.9/M*s for the S86A mutant, 2.0/M*s for the S87A mutant and 2.6/M*s for the S86A/S87A mutant enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.7
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid
-
-
0.17
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine
-
-
0.25
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
2.4
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine
-
-
42
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
-
-
0.27
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine
-
-
48
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
6.9 - 10.4
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
4 - 114
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
44 - 114
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
0.65 - 22
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
31
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid
-
-
1.51
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid
-
-
12.5
acetyl-L-Lys-D-Ala-D-Ala
pH 7.4, 25°C
0.11 - 0.3
benzoyl-D-Ala-thioglycolate
0.024 - 0.08
benzoyl-Gly-thioglycolate
0.006 - 0.08
benzoyl-Gly-thiolactate
8.3
D-Ala-D-Ala
pH 7.4, 25°C
17.5 - 350
diacetyl-L-Lys-D-Ala-D-Ala
12.8
Gly-gamma-L-Glu-D-Ala-D-Ala
-
-
69
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala
-
-
69
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine
-
-
48
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
81
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
1.41
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37°C
34.5
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine
-
-
0.9
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25°C
2.51 - 3.18
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
2.42 - 3.47
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
0.1 - 0.8
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
69
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine
-
-
1.1
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25°C
0.2 - 2.7
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
0.43 - 1.1
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
0.13 - 0.45
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
0.015 - 6.08
phenylacetyl-D-Ala-thioglycolate
0.6 - 2.2
phenylacetyl-D-Ala-thiolactate
additional information
additional information
-
6.9
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
pH 7.5, 25°C
10.4
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
pH 7.5, 25°C
4
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
pH 7.5, 25°C
114
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
pH 7.5, 25°C
44
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
106
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
114
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
0.65
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
2.2
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
22
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
-
-
0.11
benzoyl-D-Ala-thioglycolate
pH 7.2, 37°C, wild-type enzyme
0.3
benzoyl-D-Ala-thioglycolate
pH 7.2, 37°C, mutant enzyme C98A
0.024
benzoyl-Gly-thioglycolate
above, pH 7.2, 37°C, wild-type enzyme
0.08
benzoyl-Gly-thioglycolate
pH 7.2, 37°C, mutant enzyme C98A
0.006
benzoyl-Gly-thiolactate
pH 7.2, 37°C, mutant enzyme K38H
0.017
benzoyl-Gly-thiolactate
pH 7.2, 37°C, wild-type enzyme
0.08
benzoyl-Gly-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
17.5
diacetyl-L-Lys-D-Ala-D-Ala
-
Streptomyces sp. R39
55
diacetyl-L-Lys-D-Ala-D-Ala
-
Streptomyces sp. R61
267
diacetyl-L-Lys-D-Ala-D-Ala
-
54000 MW enzyme
350
diacetyl-L-Lys-D-Ala-D-Ala
-
40000 MW enzyme
2.51
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant F160A
2.68
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant R361H
2.87
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant R361A
3.02
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant Q422S
3.13
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
wild type enzyme
3.18
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
mutant Q422A
2.42
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant R361H
2.78
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant R361A
2.91
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant Q422A
2.97
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant Q422S
2.98
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant D155A
3.01
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
mutant F160A
3.47
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
wild type enzyme
0.1
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, wild-type enzyme
0.8
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
0.2
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
above, pH 7.2, 37°C, mutant enzyme C98A
0.3
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, wild-type enzyme
0.56
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
recombinant PBP 6, in 50 mM Tris-HCl, pH 8.5, at 37°C
2.7
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37°C
0.43
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
above, pH 7.2, 37°C, wild-type enzyme
1.1
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
0.13
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, mutant enzyme C98A
0.45
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
pH 7.2, 37°C, wild-type enzyme
0.015
phenylacetyl-D-Ala-thioglycolate
pH 7.2, 37°C, mutant enzyme K38H
0.5
phenylacetyl-D-Ala-thioglycolate
above, pH 7.2, 37°C, wild-type enzyme
0.87
phenylacetyl-D-Ala-thioglycolate
above, pH 7.2, 37°C, mutant enzyme C98A
6.08
phenylacetyl-D-Ala-thioglycolate
above, pH 7.2, 37°C, mutant enzyme C98A
0.6
phenylacetyl-D-Ala-thiolactate
pH 7.2, 37°C, wild-type enzyme
2.2
phenylacetyl-D-Ala-thiolactate
pH 7.2, 37°C, mutant enzyme C98A
additional information
additional information
-
-
-
additional information
additional information
-
the ratio of turnover number to Km-value for the substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala is 32/M*s for the wild-type enzyme, 3.9/M*s for the S86A mutant, 2.0/M*s for the S87A mutant and 2.6/M*s for the S86A/S87A mutant enzyme
-
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28
(3R,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1
(3R,5R,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
25
(3R,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
6.6
(3R,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
4.5
(3R,5S,6R)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
3.4
(3R,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1.3
(3S,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
16
(3S,5R,6S)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
5.4
(3S,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
3
(3S,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1
3-(acetylamino)-5-(dihydroxyboranyl)benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.034
3-(benzoylamino)-5-(dihydroxyboranyl)benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.028
3-(dihydroxyboranyl)-5-[(phenoxyacetyl)amino]benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.078
3-(dihydroxyboranyl)-5-[(thiophen-2-ylacetyl)amino]benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.032
3-(dihydroxyboranyl)-5-[(thiophen-2-ylcarbonyl)amino]benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.023
3-(dihydroxyboryl)-5-(2-methoxybenzamido)benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.088
3-(dihydroxyboryl)-5-(2-phenylacetamido)benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.4
3-(dihydroxyboryl)benzoic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
0.06
ampicillin
Corynebacterium jeikeium
-
-
1
penicillin
Streptomyces coelicolor
pH 7.2, 37°C
0.5
phenylboronic acid
Actinomadura sp. R39
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25°C
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DELTA74-90
-
deletion of the 74-90 loop markedly diminishes the deacylation rate of penicillin G with a minimal impact on acylation, and abolishes D-alanine carboxypeptidase activity
S44G
-
active site mutant, isoform PBP5, inactive, mutation changes the conformation of the dimeric state. Contrary to wild-type, the mutant only localized laterally in the cell
S63G
-
active site mutant, isoform PBP6b, inactive, mutation changes the conformation of the dimeric state
S66G
-
active site mutant, isoform PBP6a, inactive, mutation changes the conformation of the dimeric state. Contrary to wild-type, the mutant mostly avoids midcell localization
S86A
-
mutation has little effect on the interaction of the protein with penicillin G, acylation rate constants are nearly identical to wild-type enzyme. The ratio of turnover number to Km-value for the substrate N,N-diacetyl-L-Lys-D-Ala-D-Ala is 12% of the wild-type ratio
S86A/S87A
-
mutation has little effect on the interaction of the protein with penicillin G, acylation rate constants are nearly identical to wild-type enzyme. The ratio of turnover number to Km-value for the substrate N,N-diacetyl-L-Lys-D-Ala-D-Ala is 8.1% of the wild-type ratio
S87A
-
mutation has little effect on the interaction of the protein with penicillin G, acylation rate constants are nearly identical to wild-type enzyme. The ratio of turnover number to Km-value for the substrate N,N-diacetyl-L-Lys-D-Ala-D-Ala is 6.3% of the wild-type ratio
D155A
mutant, constructed for the demonstration of the relative importance of residue Asp155
F160A
mutant, constructed for the demonstration of the relative importance of residue Phe160
Q422A
mutant, constructed for the demonstration of the relative importance of residue Gln422
Q422S
mutant, constructed for the demonstration of the relative importance of residue Gln422
R361A
mutant, constructed for the demonstration of the relative importance of residue Arg361
R361H
mutant, constructed for the demonstration of the relative importance of residue Arg361
C98A
half-life at 72°C increases to 13 min, compared to 10 min for the wild-type enzyme. Half-life at 60°C increases to 220 min compared to 170 min for the wild-type enzyme. The value of the second-order acylation rate constant is not very different from those of the wild-type enzyme with the exception of the cephalotin, oxacillin and piperacillin values which are increased 5-10fold
C98N
half-life at 72°C decreases to 6 min, compared to 10 min for the wild-type enzyme. Half-life at 60°C decreases to 30 min compared to 170 min for the wild-type enzyme. The acylation rate constants by most of the beta-lactams are increased 10-70fold compared to the wild-type enzyme. The deacylation rate constant values als increases
K38H
half-life at 72°C decreases to 3.5 min, compared to 10 min for the wild-type enzyme. The mutant enzyme fails to bind beta-lactams
S96A
half-life at 72°C decreases to 8 min, compared to 10 min for the wild-type enzyme. Half-life at 60°C decreases to 120 min compared to 170 min for the wild-type enzyme. The mutant enzyme is inactive in terms of beta-lactam binding with the exception of cefoxitin, for which a residual activity is detected.The value of the second-order acylation rate constant is decreased by a factor of 3000, and that of the deacylation rate constant is decreased 120fold
W271L
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exchanging of tryptophan 271 shows no particular modification of the proteins biophysical and kinetic charcteristics
G105D
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mutation markedly impairs deacylation with only minor effects on acylation, and abolishes D-alanine carboxypeptidase activity
G105D
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mutation markedly impairs the beta-lactamase activity, with only minor effects on acylation, and promotes accumulation of a covalent complex with peptide substrates
L684P
about 50% of wild-type activity
L684P
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about 50% of wild-type activity
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additional information
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truncated forms of the enzyme
additional information
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PBP5 mutants produce cells with higher levels of pentapeptides, amidase C mutants produce higher percentage of cells in chains as compared to amidase A mutants and amidase B mutants, lack of PBP5 increases chaining of amidase mutants, reinserting of wild-type PBP5 in amidase mutants exhibits decreased chaining, amidase A mutants and amidase C mutants with lack of PBP5 produce twisted chains, additional deletion of amiB revealed extraordinarily long, convoluted and twisted chains, thus contributing to this phenotype
additional information
the addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein
additional information
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the addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein
additional information
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the addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein
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additional information
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PBP3 mutant, rings of high-molecular-weight PBPs and that of FtsZ are no longer co-localized, the co-ordination is necessary for membrane invagination
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