Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nonomuraea sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, no residual activity | Nonomuraea sp. | |
EDTA | 4 mM, complete loss of activity. Ativity may be restored in presence of 8 mM Zn2+ | Nonomuraea sp. | |
Mg2+ | 1 mM, no residual activity | Nonomuraea sp. | |
Zn2+ | 1 mM, no residual activity | Nonomuraea sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
acetyl-L-Lys-D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. | |
20 | - |
D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Nonomuraea sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | presence of 8 mM Zn2+ restores the activity of EDTA-treated enzyme | Nonomuraea sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Nonomuraea sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nonomuraea sp. | Q7WZ84 | - |
- |
Nonomuraea sp. ATCC 39727 | Q7WZ84 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | acetyl-L-Lys-D-Ala + D-Ala | - |
? | |
acetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | acetyl-L-Lys-D-Ala + D-Ala | - |
? | |
D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | D-Ala + D-Ala | - |
? | |
D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | D-Ala + D-Ala | - |
? | |
additional information | on substrates mimicking peptidoglycan precursors, isoform VanYn shows D,D-carboxypeptidase and D,D-dipeptidase activity, but lacks D,D-carboxyesterase ability on D-Ala-D-Lac-terminating peptides | Nonomuraea sp. | ? | - |
? | |
additional information | on substrates mimicking peptidoglycan precursors, isoform VanYn shows D,D-carboxypeptidase and D,D-dipeptidase activity, but lacks D,D-carboxyesterase ability on D-Ala-D-Lac-terminating peptides | Nonomuraea sp. ATCC 39727 | ? | - |
? | |
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala | - |
? | |
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Nonomuraea sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. | |
12.5 | - |
acetyl-L-Lys-D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.415 | - |
D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. | |
1.56 | - |
acetyl-L-Lys-D-Ala-D-Ala | pH 7.4, 25°C | Nonomuraea sp. |