Literature summary for 3.4.16.4 extracted from

Macheboeuf, P.; Lemaire, D.; Martins, A.D.; Dideberg, O.; Jamin, M.; Dessen, A.
Trapping of an acyl-enzyme intermediate in a penicillin-binding protein (PBP)-catalyzed reaction (2008), J. Mol. Biol., 376, 405-413.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified Pbp1b in complex with N-benzoyl-D-Ala or pseudo-substrate N-benzoyl-D-alanylmercaptoacetic acid thioester, hanging drop vapor diffusion method, mixing of 0.001 ml of 6-10 mg/ml protein and 5 mM N-benzoyl-D-alanylmercaptoacetic acid thioester, with 0.001 ml of well solution containing 50 mM HEPES, pH 7.0, 0.8 M (NH4)2SO4, 2.8 M NaCl at 15°C, X-ray difraction structure determination and analysis at 2.5 A resolution, molecular replacement	Streptococcus pneumoniae

Crystallization (Comment)

Organism

purified Pbp1b in complex with N-benzoyl-D-Ala or pseudo-substrate N-benzoyl-D-alanylmercaptoacetic acid thioester, hanging drop vapor diffusion method, mixing of 0.001 ml of 6-10 mg/ml protein and 5 mM N-benzoyl-D-alanylmercaptoacetic acid thioester, with 0.001 ml of well solution containing 50 mM HEPES, pH 7.0, 0.8 M (NH4)2SO4, 2.8 M NaCl at 15°C, X-ray difraction structure determination and analysis at 2.5 A resolution, molecular replacement

Streptococcus pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
nitrocefin	-	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Streptococcus pneumoniae	low molecular mass PBPs can exert carboxypeptidase and/or endopeptidase activities and play auxiliary roles in the peptidoglycan metabolism process	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	low molecular mass PBPs can exert carboxypeptidase and/or endopeptidase activities and play auxiliary roles in the peptidoglycan metabolism process	Streptococcus pneumoniae	?	-	?
additional information	the serine protease catalyzes glycosyl transfer, i.e. polymerization of glycan chains, and transpeptidation, i.e. cross-linking of stem peptides, which are essential for peptidoglycan stability in bacterial cell wall synthesis, and for the cell division process, overview	Streptococcus pneumoniae	?	-	?
N-acetyl-L-Lys-D-Ala-D-Ala + H2O	-	Streptococcus pneumoniae	N-acetyl-L-Lys-D-Ala + D-Ala	-	?
N-benzoyl-D-Ala + H2O	substrate binding structure	Streptococcus pneumoniae	benzoate + D-Ala	-	?

Subunits

Subunits	Comment	Organism
More	PBP1b structure analysis, overview	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
PBP	-	Streptococcus pneumoniae
PBP1b	-	Streptococcus pneumoniae
penicillin-binding protein	-	Streptococcus pneumoniae