Information on EC 2.4.1.173 - sterol 3beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.173
-
RECOMMENDED NAME
GeneOntology No.
sterol 3beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:sterol 3-O-beta-D-glucosyltransferase
Not identical with EC 2.4.1.192 (nuatigenin 3beta-glucosyltransferase) or EC 2.4.1.193 (sarsapogenin 3beta-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
123940-38-5
-
9075-00-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
ugt80A1
SwissProt
Manually annotated by BRENDA team
ugt51c1
SwissProt
Manually annotated by BRENDA team
synonym Colletotrichum lagenarium, strain 104-T
-
-
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
ugt52
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
gene ATG26
SwissProt
Manually annotated by BRENDA team
ugt51b1
SwissProt
Manually annotated by BRENDA team
gene PGUG_04770
UniProt
Manually annotated by BRENDA team
gene PGUG_04770
UniProt
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
gene ATG26
UniProt
Manually annotated by BRENDA team
pea
-
-
Manually annotated by BRENDA team
or Candida bogoriensis
-
-
Manually annotated by BRENDA team
white mustard
-
-
Manually annotated by BRENDA team
cv Black Beauty, eggplant
-
-
Manually annotated by BRENDA team
potato
-
-
Manually annotated by BRENDA team
gene WsSGTL1
-
-
Manually annotated by BRENDA team
mutant defective in the orthologue of UGT51, encoding sterol glucosyltransferase
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
-
alteration of the membrane physical state caused by heat stress may be linked to activate sterol glucosyltransferase to form cholesteryl glucoside
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose
?
show the reaction diagram
-
demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose
?
show the reaction diagram
-
nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose
?
show the reaction diagram
-
tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose
?
show the reaction diagram
-
hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose
?
show the reaction diagram
-
isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose
?
show the reaction diagram
-
diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose
?
show the reaction diagram
-
tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
24-methylene cholesterol + UDP-glucose
UDP + 24-methylene-cholesterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose
3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP
show the reaction diagram
-
-
-
-
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
show the reaction diagram
5alpha-cholest-7-en-3beta-ol + UDP-glucose
?
show the reaction diagram
-
14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
5alpha-cholestan-3beta-ol + UDP-glucose
?
show the reaction diagram
-
cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
5alpha-cholestan-3beta-ol + UDP-glucose
UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
androst-5-en-3beta-ol-17-one + UDP-glucose
?
show the reaction diagram
-
androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
show the reaction diagram
brassicasterol + UDP-glucose
UDP + brassicasterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
cholest-5-en-22-on-3beta-ol + UDP-glucose
?
show the reaction diagram
-
22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,19-diol + UDP-glucose
?
show the reaction diagram
-
9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,20alpha-diol + UDP-glucose
?
show the reaction diagram
-
90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,25-diol + UDP-glucose
?
show the reaction diagram
-
25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta-ol + UDP-glucose
?
show the reaction diagram
-
cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta-ol-7-one + UDP-glucose
?
show the reaction diagram
-
5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
show the reaction diagram
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
show the reaction diagram
diosgenin + UDP-glucose
UDP + diosgenin 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
ergosterol + UDP-glucose
UDP + ergosterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
pregn-5-en-3beta-ol-20-one + UDP-glucose
?
show the reaction diagram
-
pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
pregnenolene + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
pregnenolone + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
solasodine + UDP-glucose
UDP + solasodine 3-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
stigmasta-5,22-dien-3beta-ol + UDP-glucose
?
show the reaction diagram
-
stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose
?
show the reaction diagram
-
82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmastan-3beta-ol + UDP-glucose
?
show the reaction diagram
-
38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
?
show the reaction diagram
-
-
-
?
UDP-glucose + 3beta-hydroxy-pregna-5,16-dien-one
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
UDP-glucose + 4-demethyl-obtusifoliol
UDP + 4-demethyl-obtusifoliol 3-beta-glucoside
show the reaction diagram
-
58% of the activity with sitosterol
-
?
UDP-glucose + 5-alpha-cholestanol
UDP + 5-alpha-cholestanol 3-beta-D-glucoside
show the reaction diagram
-
8% of the activity with sitosterol
-
?
UDP-glucose + 5-cholestenol
UDP + 5-cholestenol 3-beta-D-glucoside
show the reaction diagram
-
12% of the activity with sitosterol
-
?
UDP-glucose + 7-dehydrocholesterol
UDP + 7-dehydrocholesterol 3-beta-D-glucoside
show the reaction diagram
-
6% of the activity with sitosterol
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + alpha-spinasterol
UDP + alpha-spinasterol 3-beta-D-glucoside
show the reaction diagram
-
31% of the activity with sitosterol
-
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + dehydroepiandrosterone
UDP + dehydroepiandrosterone 3-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + epiandrosterone
UDP + epiandrosterone 3-beta-glucoside
show the reaction diagram
-
best substrate
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + isofucosterol
UDP + isofucosterol 3-beta-glucoside
show the reaction diagram
-
73% of the activity with sitosterol
-
?
UDP-glucose + poriferasterol
UDP + poriferasterol 3-beta-glucoside
show the reaction diagram
-
synthesis of poriferasterol monoglucoside may be involved in differentiation
-
-
-
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-glucoside
show the reaction diagram
-
57% of the activity with epiandrosterone
-
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + solasodine
UDP + solasodine 3-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + testosterone
UDP + testosterone 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
show the reaction diagram
UDP-glucose + poriferasterol
UDP + poriferasterol 3-beta-glucoside
show the reaction diagram
-
synthesis of poriferasterol monoglucoside may be involved in differentiation
-
-
-
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
inhibitory effect at 1 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Hydroxy-5-nitrobenzyl bromide
-
-
2-mercaptoethanol
slight inhibition of the isozyme
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
-
-
4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid
-
-
4-thio-UDP
-
50% of inhibition at 160 uM
5-Hg UDP
-
-
ATP
-
inhibition at 1-3 mM but slightly stimulation at low concentration
Cetyltrimethylammonium bromide
-
strong inhibition
cholesteryl glucoside
-
-
coprostanol
diacylglycerol
-
with saturated fatty acids
diethyldicarbonate
-
protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose
epicholestanol
GDP-glucose
-
20% of inhibition at high concentration
GTP
-
the inhibitory effect of GTP is less prominent as compared to UDP
Hg2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5
iodoacetamide
-
strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
maleate buffer
-
strong inhibition at 50 mM
-
Mersalyl
-
50% of inhibition at 40 uM
monoacylglycerol
-
with saturated fatty acids
N-ethylmaleimide
n-octyl thioglucoside
-
irreversible inhibited, 50% of activity at 4.8 mM
NH4+
-
inhibited by
Ni2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.2
p-chloromercuribenzenesulfonic acid
-
-
p-chloromercuribenzoate
p-mercuribenzenesulfonate
-
50% of irreversible inhibition at 0.5 mM, UDP and UDPG-glucose protect from inhibition of 10 mM
Phenylglyoxal
-
92% of inhibition at 10 mM
Phosphate buffer
-
strong inhibition at 50 mM
-
pyridoxal 5'-phosphate
-
-
Sodium deoxycholate
sodium taurocholate
triacylglycerol
-
with saturated fatty acids
Triton
strong inhibition of isozyme GhSGT1
Triton X-100
Tween 20
-
strong inhibition at concentrations below 0.05%
Tween 60
-
strong inhibition at concentrations below 0.05%
UDP-2',3'-dialdehyde
-
inhibits irreversibly the enzyme
UDP-mannose
-
50% of inhibition at 40 uM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
DIECA
-
activated by
dithiothreitol
-
25% stimulation at 1 mM
EGTA
-
50% of inhibition at 13 mM
Emulphogen BC-720
-
stimulation al low concentrations
-
ethanol
-
increase of activity at 7-20%
KOH
-
treatment with KOH pH 10.4 increase activity about 65%, hydroxyl ions can be used in place of detergents to reveal latent enzymatic sites
lecithin
p-chloromercuribenzoate
-
potent inhibitory effect indicating that free SH groups are essential for activity
phosphatidylcholine
phosphatidylethanolamine
-
increases 125% of activity
phosphatidylglycerol
-
increases Vmax
phosphatidylserine
-
33% increase of activity
phospholipase
-
Phospholipids
Triton
activates isozyme GhSGT2
Triton N-101
-
stimulation al low concentrations
-
Triton X-100
Triton X-114
-
stimulation al low concentrations
Tween-20
-
2fold activity stimulation at 0.1%
Tween-40
-
70% activity stimulation at 0.05%
Tween-60
-
35% activity stimulation at 0.1%
additional information
-
stimulated by acylglycerols containing unsaturated fatty acid residues
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
24-methylene cholesterol
-
for the cytosolic enzyme
0.006
3-beta-hydroxy-pregna-5,16-dien-20-one
-
for the cytosolic enzyme
0.017
3beta-hydroxy-16,17-alpha-epoxypregnenolene
-
-
0.01
3beta-hydroxy-16,17alpha-epoxypregnenolone
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.007
3beta-hydroxy-pregna-5,16-dien-20-one
-
-
0.01
3beta-hydroxy-pregna-5,16-dien-one
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.063
5alpha-Cholestan-3beta-ol
-
-
0.005 - 0.059
beta-sitosterol
0.0045 - 0.03
brassicasterol
0.056 - 0.0672
cholesterol
0.04 - 0.119
dehydroepiandrosterone
0.002
diosgenin
-
for the cytosolic enzyme
0.004 - 0.035
ergosterol
0.0048
poriferasterol
-
-
0.005 - 0.008
pregnenolone
0.005 - 0.54
sitosterol
0.003 - 0.007
solasodine
0.00143
stigmast-5-en-3beta-ol
-
pH 7,3, 30°C
0.027 - 0.125
stigmasterol
0.8379
testosterone
pH 7.8, 30°C
0.025 - 0.059
transandrosterone
0.008 - 0.34
UDP-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.38
24-methylene cholesterol
Withania somnifera
-
for the cytosolic enzyme
0.0093
3-beta-hydroxy-pregna-5,16-dien-20-one
Withania somnifera
-
for the cytosolic enzyme
0.0037
beta-sitosterol
Withania somnifera
-
for the cytosolic enzyme
475 - 650.6
cholesterol
0.0072
dehydroepiandrosterone
Withania somnifera
-
for the cytosolic enzyme
0.0023
diosgenin
Withania somnifera
-
for the cytosolic enzyme
0.0047
pregnenolone
Withania somnifera
-
for the cytosolic enzyme
0.0088
stigmasterol
Withania somnifera
-
for the cytosolic enzyme
351.9
testosterone
Ogataea angusta
A7KAK6
pH 7.8, 30°C
0.0087
UDP-glucose
Withania somnifera
-
for the cytosolic enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00721 - 0.0097
cholesterol
189
0.00042
testosterone
Ogataea angusta
A7KAK6
pH 7.8, 30°C
284
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
5-Hg UDP
-
reversible inhibitor
0.047
UDP
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.53 - 1.3
Co2+
0.5
Hg2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5
1.2
Ni2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.2
0.065
UDP
Solanum melongena
-
pH 7.3, 30°C
0.035
UTP
Solanum melongena
-
pH 7.3, 30°C
0.04 - 0.09
Zn2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
the enzyme has an optimum pH for activity around 8.0 with half maximal activities at 7.0 and above 9.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8
-
determined by chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
from 4 weeks old plants, expressend in significantly lower level than in leaves and roots
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
during micropexophagy, the glucosyltransferase is associated with the micropexophagic apparatus. GRAM domain is essential for proper protein association with its target membrane
-
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
SDS-PAGE
54200
x * 54200, about, sequence calculation
55900
x * 55900, about, sequence calculation
56000
-
purification by Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE
61800
x * 61800, about, sequence calculation
66000
-
cloned, N-terminal peptide of 133 amino acid could be cleaved resulting in a molecular weight of 52000 in agreement with 56000 of the purified enzyme
69000
cloned, calculated molecular mass
70000
-
gel filtration
72000
-
gel filtration
114000
cloned, calculated molecular mass
136000
140000
-
by gel filtration
171000
cloned, calculated molecular mass
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
Tris-HCl 0.1 M, Triton X-100 0.3%
637771
7.7
-
phosphate buffer 0.1 M
637769
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethanol
-
increases activity between 7-15% but decreases activity above 20%
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 C, Tris-HCl 0.1 M, pH 7.2, two weeks
-
-20 C, Tris-HCl 0.1 M, pH 7.5, several weeks
-
2C, Tris-HCl 50 mM, pH 8.5, two weeks 75% of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cibacron Blue column, Sephadex G-25 column and Mono Q anion exchange column
-
gel filtration and ion exchange column chromatography
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partial purification by DEAE-cellulose column DE-52
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partial purification by differential centrifugation
partial purification by differential centrifugation and DEAE-cellulose column
partial purification by differential centrifugation and gel filtration on Sephadex G-100
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partial purification by differential centrifugation and precipitation with Me2CO
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partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B
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partial purification by differential centrifugation and Sephadex G-150 column
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partially purified by gel filtration chromatography, resutlts in 3.73fold purification
purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE
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recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain DH5alpha
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expression in Escherichia coli
expression in Escherichia coli strain XJb
gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern; gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control; gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GhSGT1, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2; gene GhSGT2, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2
gene PGUG_04770, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene WsSGTL1, ectopic overexpression in Withania somnifera using Agrobacterium tumefaciens transfection method, quantitative real-time PCR enzyme expression analysis. Overexpression of WsSGTL1 gene which is responsible for increase in glycosylated withanolide and sterols, and confers better growth and tolerance to both biotic and abiotic stresses
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protein encoded by ugt51E1 is expressed in Escherichia coli
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the open reading frame of SGTL1 is expressed in Escherichia coli strain DH5alpha
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y642P
amino acid substitution within the GRAM domain abolished this association as well as micropexophagy
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
Show AA Sequence (580 entries)
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