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Literature summary for 2.4.1.173 extracted from

  • Potocka, A.; Zimowski, J.
    Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose:sterol glucosyltransferase (2008), Acta Biochim. Pol., 55, 127-134.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Solanum melongena
additional information stimulated by acylglycerols containing unsaturated fatty acid residues Solanum melongena
Triton X-100 130fold at 0.1% Solanum melongena

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzyl bromide
-
Solanum melongena
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
-
Solanum melongena
4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid
-
Solanum melongena
cholesteryl glucoside
-
Solanum melongena
Co2+
-
Solanum melongena
diacylglycerol with saturated fatty acids Solanum melongena
diethyldicarbonate protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose Solanum melongena
monoacylglycerol with saturated fatty acids Solanum melongena
additional information inhibited at high ionic strength, 50% inhibition at 0.52 M NaF or 0.29 M NaCl; inhibited by dicarboxylic amino acids-modifying reagent Woodward’s Reagent K protected against inactivation upon pre-incubation with UDP-glucose but not with stigmast-5-en-3beta-ol; no effect with Mg2+, Ca2+, Mn2+, Tween20, Tween 40, Tween 60, Tween 80, Tyloxapol, sodium deoxycholate, EDTA, EGTA, and reagents modifying serine, arginine and tyrosine side chains Solanum melongena
N-ethylmaleimide protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose Solanum melongena
n-octyl thioglucoside irreversible inhibited, 50% of activity at 4.8 mM Solanum melongena
p-chloromercuribenzenesulfonic acid
-
Solanum melongena
pyridoxal 5'-phosphate
-
Solanum melongena
triacylglycerol with saturated fatty acids Solanum melongena
UDP
-
Solanum melongena
UTP
-
Solanum melongena
Zn2+
-
Solanum melongena

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00143
-
stigmast-5-en-3beta-ol pH 7,3, 30°C Solanum melongena
0.01429
-
UDP-glucose pH 7.3, 30°C Solanum melongena

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Solanum melongena 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum melongena may participate in the regulating of free sterol concentration in the cell ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum melongena
-
cv Black Beauty, eggplant
-

Purification (Commentary)

Purification (Comment) Organism
partial Solanum melongena

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Solanum melongena
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
(25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
5alpha-cholest-7-en-3beta-ol + UDP-glucose 14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
5alpha-cholestan-3beta-ol + UDP-glucose cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
androst-5-en-3beta-ol-17-one + UDP-glucose androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-22-on-3beta-ol + UDP-glucose 22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-3beta,19-diol + UDP-glucose 9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-3beta,20alpha-diol + UDP-glucose 90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-3beta,25-diol + UDP-glucose 25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-3beta-ol + UDP-glucose cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
cholest-5-en-3beta-ol-7-one + UDP-glucose 5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
additional information may participate in the regulating of free sterol concentration in the cell Solanum melongena ?
-
?
additional information UDP-galactose, TDP-glucose and CDP-glucose are also suitable as a sugar source for sterol glucosylation but with distinctly lower rates Solanum melongena ?
-
?
additional information no activity with solasodine (25R-22alphaN-spirosol-5-enin-3beta-ol), solanidine (22S,25S-solanid-5-enin-3beta-ol), sarsasapogenin (25S-5beta-spirostan-3beta-ol), yamogenin (25S-spirost-5-en-3beta-ol), thiocholesterol (cholest-5-en-3beta-tiol), 5beta-cholestan-3beta-ol (coprostanol), 5alpha-cholestan-3alpha-ol (epicholestanol), 5beta-cholestan-3alpha-ol (epicoprostanol), lanosterol (4,4’,14-trimethyl-5alpha-cholest-8-en-3beta-ol), and beta-amyrin (olean-12-en-3beta-ol) do not serve as glucose acceptors Solanum melongena ?
-
?
pregn-5-en-3beta-ol-20-one + UDP-glucose pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
stigmast-5-en-3beta-ol + UDP-glucose sitosterol, 100% of the activity Solanum melongena additional information 3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products ?
stigmasta-5,22-dien-3beta-ol + UDP-glucose stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose 82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?
stigmastan-3beta-ol + UDP-glucose 38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
?

Synonyms

Synonyms Comment Organism
UDP-glucose:sterol glucosyltransferase
-
Solanum melongena

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.035
-
pH 7.3, 30°C Solanum melongena UTP
0.065
-
pH 7.3, 30°C Solanum melongena UDP
0.09
-
pH 7.3, 30°C Solanum melongena Zn2+
0.53
-
pH 7.3, 30°C Solanum melongena Co2+