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Literature summary for 2.4.1.173 extracted from

  • Wojciechowski, Z.A.; Zimowski, J.; Zimowski, J.G.; Lyznik, A.
    Specificity of sterol-glucosylating enzymes from Sinapis alba and Physarum polycephalum (1979), Biochim. Biophys. Acta, 570, 363-370.
    View publication on PubMed

Application

Application Comment Organism
synthesis
-
Physarum polycephalum
synthesis synthesis of steryl beta-D-monoglucosides that are constituents of the cell membrane of higher plants Sinapis alba

Inhibitors

Inhibitors Comment Organism Structure
coprostanol potent inhibitor Physarum polycephalum
coprostanol not inhibitory Sinapis alba
epicholestanol potent inhibitor Physarum polycephalum
epicholestanol not inhibitory Sinapis alba
sodium deoxycholate
-
Sinapis alba
sodium taurocholate
-
Sinapis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
sitosterol
-
Sinapis alba
0.08
-
UDP-glucose
-
Sinapis alba

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Physarum polycephalum 16020
-
membrane organelles membrane Sinapis alba 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Physarum polycephalum
Ca2+ not stimulated between 0.001 mM to 10 mM Sinapis alba
Hg2+
-
Physarum polycephalum
Hg2+ 50% of inhibition at 0.4 mM Sinapis alba
Mg2+
-
Physarum polycephalum
Mg2+ not stimulated between 0.001 mM to 10 mM Sinapis alba
Zn2+
-
Physarum polycephalum
Zn2+ 50% of inhibition at 0.006 mM Sinapis alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
by gel filtration Sinapis alba

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-
Sinapis alba
-
white mustard
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and Sephadex G-150 column Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
cell culture mycoplasma strain M3C IV Physarum polycephalum
-
seedling
-
Sinapis alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is absolute specific to UDP-glucose Sinapis alba ?
-
?
additional information glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates Physarum polycephalum ?
-
?
additional information glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates Sinapis alba ?
-
?
additional information enzyme can use CDP-glucose and TDP-glucose at 6 times slower rate Physarum polycephalum ?
-
?
additional information coprostanol and epicholestanol are not glucosylated Physarum polycephalum ?
-
?
additional information all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation Physarum polycephalum ?
-
?
additional information all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation Sinapis alba ?
-
?
UDP-glucose + campesterol
-
Physarum polycephalum UDP + campesterol 3-beta-D-glucoside
-
?
UDP-glucose + campesterol best substrate Sinapis alba UDP + campesterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol
-
Sinapis alba UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol best substrate Physarum polycephalum UDP + sitosterol 3-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Physarum polycephalum
30
-
assay at Sinapis alba

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.3
-
Tris-HCl 0.1 M, Triton X-100 0.3% Sinapis alba