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Literature summary for 2.4.1.173 extracted from

  • Warnecke, D.C.; Heinz, E.
    Purification of a membrane-bound UDP-glucose:sterol beta-D-glucosyltransferase based on its solubility in diethyl ether (1994), Plant Physiol., 105, 1067-1073.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
4-thio-UDP 50% of inhibition at 160 uM Avena sativa
UDP 50% of inhibition at 70 uM Avena sativa
UDP-mannose 50% of inhibition at 40 uM Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24 0.54 sitosterol
-
Avena sativa
0.34
-
UDP-glucose
-
Avena sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Avena sativa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
purification by Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE Avena sativa

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
shoot etiolated Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDP-glucose is an effective glucose donor but not so UDP-mannose, UDP-galactose or UMP-glucose Avena sativa ?
-
?
UDP-glucose + cholesterol with sitosterol, best substrates Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol 69% of the activity with sitosterol Avena sativa UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol with cholesterol, best substrates Avena sativa UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol 63% of the activity with sitosterol Avena sativa UDP + stigmasterol 3-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Avena sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
Tris-HCl 60 mM, Triton X-100 0.2%, dithiothreitol 2.5 mM Avena sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.047
-
UDP
-
Avena sativa