Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-thio-UDP | 50% of inhibition at 160 uM | Avena sativa | |
UDP | 50% of inhibition at 70 uM | Avena sativa | |
UDP-mannose | 50% of inhibition at 40 uM | Avena sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | 0.54 | sitosterol | - |
Avena sativa | |
0.34 | - |
UDP-glucose | - |
Avena sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Avena sativa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
purification by Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE | Avena sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | etiolated | Avena sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UDP-glucose is an effective glucose donor but not so UDP-mannose, UDP-galactose or UMP-glucose | Avena sativa | ? | - |
? | |
UDP-glucose + cholesterol | with sitosterol, best substrates | Avena sativa | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | 69% of the activity with sitosterol | Avena sativa | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | with cholesterol, best substrates | Avena sativa | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | 63% of the activity with sitosterol | Avena sativa | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Avena sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
Tris-HCl 60 mM, Triton X-100 0.2%, dithiothreitol 2.5 mM | Avena sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
UDP | - |
Avena sativa |