Application | Comment | Organism |
---|---|---|
synthesis | synthesis of poriferasterol monoglucoside may be involved in differentiation | Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
poriferasterol | - |
Physarum polycephalum | |
0.12 | - |
UDP-glucose | - |
Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Physarum polycephalum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Physarum polycephalum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + poriferasterol | Physarum polycephalum | synthesis of poriferasterol monoglucoside may be involved in differentiation | UDP + poriferasterol 3-beta-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by differential centrifugation and gel filtration on Sephadex G-100 | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain F and J activity only in diploid cells | Physarum polycephalum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + campesterol | 80% of the activity with poriferasterol | Physarum polycephalum | UDP + campesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | 12% of the activity with poriferasterol | Physarum polycephalum | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | 9% of the activity with poriferasterol | Physarum polycephalum | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + poriferasterol | synthesis of poriferasterol monoglucoside may be involved in differentiation | Physarum polycephalum | UDP + poriferasterol 3-beta-glucoside | - |
? | |
UDP-glucose + sitosterol | maximal activity, the same as poriferasterol | Physarum polycephalum | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | 14% of the activity with poriferasterol | Physarum polycephalum | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Physarum polycephalum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
Tris-HCl 0.1 M | Physarum polycephalum |