Information on EC 2.1.1.34 - tRNA (guanosine18-2'-O)-methyltransferase

Word Map on EC 2.1.1.34
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.34
-
RECOMMENDED NAME
GeneOntology No.
tRNA (guanosine18-2'-O)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanosine18-2'-O)-methyltransferase
The enzyme catalyses the methylation of guanosine18 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-01-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CA274
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
show the reaction diagram
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
show the reaction diagram
-
involved in post-transcriptional tRNA modification
-
-
-
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
show the reaction diagram
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-AGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-AGA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-dG18 mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-dG18 mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GAA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GAA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GGA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GUA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GUA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + 2-methylthio N6(gamma,gamma,dimethylallyl)adenosine in tRNA
show the reaction diagram
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
show the reaction diagram
S-adenosyl-L-methionine + yeast tRNA-Phe
S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
show the reaction diagram
-
involved in post-transcriptional tRNA modification
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
activation, 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DELTAD-arm
-
tRNAPhe variant from yeast with a modification in the D-stem, inhibition
-
G18A
-
tRNAPhe variant from yeast with an exchange of G to A at position 18, inhibition
-
S-adenosyl-L-homocysteine
-
strong, product inhibition, competitive
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
enhances the affinity for tRNA and induces formation of the tetramer structure
spermine
-
activation, 5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000006
Escherichia coli tRNAIle
-
-
-
0.000005
Escherichia coli tRNAMet
-
-
-
0.00001
Escherichia coli tRNAPhe
-
-
-
0.021 - 0.27
guanosine18 in tRNATyr
-
0.00011 - 0.0013
guanosine18 in yeast tRNAPhe
-
0.000082
guanosine18 in yeast tRNAPhe mutant transcript
-
pH and temperature not specified in the publication, Vmax: 3.1 micromol/mg/h
-
0.0125
guanosine18 in yeast tRNAPhe-AGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.00675 micromol/mg/h
-
0.00082
guanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.1 micromol/mg/h
-
0.0002
guanosine18 in yeast tRNAPhe-GAA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.135 micromol/mg/h
-
0.000245
guanosine18 in yeast tRNAPhe-GGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 1.2 micromol/mg/h
-
0.0003
guanosine18 in yeast tRNAPhe-GUA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.18 micromol/mg/h
-
0.00047 - 28
S-adenosyl-L-methionine
0.000005 - 0.00001
yeast tRNAPhe
-
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00205
DELTAD-arm
-
-
-
0.00079
G18A
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
45
-
Bacillus subtilis tRNALeu
55
-
Bacillus subtilis tRNAThr
additional information
-
optimal temperature for methylation is ca. 20°C lower than the melting temperature of the tRNA, overview
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
-
strain HB 8 shows 80% activity at 40°C, strain HB 27 shows 60% activity
52 - 79
-
at 67°C content of the TrmH protein is ca. 1/5000 of the total proteins, content of the TrmH protein in 52°C cultured cells slightly decreases as compared with that in 67°C cultured cells, TrmH contents in cells cultured at 75 or 79°C are similar to that at 67°C
55 - 68
-
about half-maximal activity at 55°C and 68°C, yeast tRNAPhe
55 - 75
-
about half-maximal activity at 55°C and 75°C, Escherichia coli tRNAfMet
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aquifex aeolicus (strain VF5)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gel filtration
54000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by the hanging-drop method, at 1.85 A resolution, diffraction-quality crystals in the pH range 4.0–4.5, enzyme can not be crystallized with S-adenosyl-L-methionine or an analog in the active site
in complex with S-adenosyl-L-methionine
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
Thermus thermophilus strain HB27: 40% of maximal activity, strain HB8: 80% of maximal activity
60
-
stable below, tRNAPhe
70
-
stable below, tRNAMet
90
-
t1/2: 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
urea, stable to 6 M
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30 °C, Tris-HCl buffer, pH 7.5, 50% glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea
-
gel filtration, more than 99% pure
native and recombinant from Escherichia coli
-
wild-type and mutants purified by gel filtration, mutants R11A and R166A purified by repeated ion-exchange chromatography, to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis of spoU gene, encodes tRNA (Gm18) 2'-O-methyltransferase
-
expressed in Escherichia coli
-
expression in Escherichia coli with and without His-tag, amino acid sequence analysis and comparison
-
expression inb Escherichia coli
into vector pThioHisB and transformed into Escherichia coli strain DH5alpha, overexpressed in Escherichia coli Rosetta(DE3)/pLysS cells
vector pET30aGm expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D180A
-
42% of wild-type activity
D190A
-
5% of wild-type activity
E124D
-
small increase in Km-value
E124Q
-
no enzymic activity
E182A
-
29% of wild-type activity
H34A
-
no change of affinity for S-adenosyl-L-methionine and tRNA
H71A
-
has increased affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
I142A
-
kinetic parameters comparable to wild-type
K186A
-
7% of wild-type activity
K32A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
K90A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
L101A
-
13fold increase in Km-value
L151A
-
5fold increase in Km-value
L179Stop
M144A
-
15fold increase in Km-value
N152D
-
no enzymic activity
N152E
-
no enzymic activity
N35A
-
marked decrease in methyl transfer activity
N35D
-
marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine
N35Q
-
dramatic increase in Km-value
P143Q
-
small increase in Km-value
Q185A
-
16% of wild-type activity
R109A
-
no change of affinity for S-adenosyl-L-methionine and slightly increased affinity for tRNA
R11A
-
has lost its affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R14M
-
dissociation of subunits consistent with that from the wild-type enzyme
R166A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R168A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R176A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R19A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R41A
-
no enzymic activity
R41K
-
30fold increase in Km-value
R41M
-
large increase in Km-value
R8A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
S150A
-
large increase in Km-value
S150C
-
Km-value similar to wild-type, decrease in Vmax
S150T
-
4fold increase in Km-value
S37G
-
5fold increase in Km-value
T99A
-
small increase in Km-value
V128A
-
small increase in Km-value
V187A
-
11% of wild-type activity
Y184A
-
10% of wild-type activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
Show AA Sequence (2359 entries)
Please use the Sequence Search for a specific query.